During our assignment to the laboratory unit, we observed flotation method of fecal sample examination where the procedure includes, thorough mixing of the fecal sample with water and only the liquid supernatant is filtered for further processing. A drop of supernatant is placed on the slide using a pipette and then observed under a microscope.
When observed under the microscope(10x) we observed eggs of Toxocara canis indicating the dog was infected with the parasite. The lab technician stated that the visibility or absence of parasitic ova or larvae depends on parasite load, life cycle stage, and sample quality.
This explains why some clinically suspected parasitic cases may still show negative results, emphasizing the importance of repeat testing and correlation with clinical sign.