In vitro phytoconsituents investigation, antioxidant capacity, antimicrobial activity and in vivo anticancer activity of Mucuna poggei fruit peel extracts obtained by different extraction approaches

In vitro phytoconsituents investigation, antioxidant capacity, antimicrobial activity and in vivo anticancer activity of Mucuna poggei fruit peel extracts obtained by different extraction approaches

Md. Jakir Hossain^a , Shashwata Biswas^b , Sohidul Islam^a , Mohammad Shahriar^c , and Chowdhury Rafiqul Ahsan^b*

^a Department of Biochemistry and Microbiology, North South University, Dhaka 1229,

Bangladesh.

^b Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh.

^c Department of Pharmacy, University of Asia Pacific, Dhaka 1215, Bangladesh

Keywords:

Mucuna poggei, phytochemical, antioxidant, ehrlich ascites carcinoma

Abstract

Mucuna poggei plant belongs to family Fabaceae and typically found in tropical woodlands, is used against many diseases. However, still many of its information retarding biochemical and antimicrobial profiles are insufficient. This experiment was performed to observe the phytochemical components, antioxidant capacity, antimicrobial activity, and in vivo anticancer activity of M. poggei fruit peel extracts. By soxhlet apparatus, the Mucuna poggei fruit peel extracts were obtained using four solvents (n-hexane, petroleum benzene, chloroform, and methanol) according to their increasing solubility. In in vitro biological activity, phytochemical components, antioxidant capacity and antibacterial activity was observed using standard protocol. Mice from each group (n=6) of positive (bleomycin treated), negative (Tween 20 treated) and experimental (sample extracts treated) were seeded approximately 1x105 ehrlich ascites carcinoma (EAC) cells into the peritoneal cavity. EAC cell reducing capacity was observed after seven consecutive days of treatment and therefore, percent increase in life span (%ILS) was calculated. Carbohydrates, glycosides, tannins, saponins, resins, phenolic compounds, proteins, and steroids were found to be present. Sample extracts showed the significant results (p < 0.01) in antioxidant capacity experiments. An intermediate zone of sensitivity of methanol extract was found against Escherichia coli, Shigella flexneri, and Staphylococcus aureus when compared to amoxicillin. In anticancer activity experiment the negative and positive control groups showed an average cell count of 2.4x108 and 1.9x108 respectively and the experimental group showed the cell count of 2.2x 108 , 2.1x108 , 1.8x108 , and 1.29x108 when mice received hhexane, petroleum benzene, chloroform, and methanol extract respectively. Mice were then kept for survival until the death of all mice and the positive control showed %ILS of 15.1 where experimental groups showed %ILS of 4.31, 7.92, 11.51, and 33.1 when mice received nhexane, petroleum benzene, chloroform, and methanol respectively. On the other hand, when mice received the methanol extracts in a cyclic manner then the %ILS found to be 66.69 for experimental group and 35.79 for positive control. All these results indicate the potential of M. poggei fruit peel extracts as an antimicrobial, antioxidant and anticancer agent in the experimental models.

Citation:

Hossain Md. J., Biswas S., Islama S., Shahriar M., and Ahsan C.R., 2019. In vitro phytoconsituents investigation, antioxidant capacity, antimicrobial activity and in vivo anticancer activity of Mucuna poggei fruit peel extracts obtained by different extraction approaches. Turkish Journal of Biotechnology. Photon 102, 120-129

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Photon Ignitor: e-ISJNA43723162D8949

Conclusion

From above result, it can be concluded that mainly methanol extract of M. poggei fruit peel has the potent antioxidant capacity and in vivo anticancer effect to some extent in terms of the capacity of the extract to reduce intra peritoneal cancer cell count and increased survival of the experimental animal. Further study should include isolation of pharmacologically active ingredients from M. poggei fruit peel and evaluation of each active ingredient.

Research Highlights

M. poggei contains phytoconostituents along with notable range of CUPRAC and antioxidant. The fruit peel of this plant has tumor cell (EAC) killing activity. Tumor bearing mice survives more than negative and positive control groups if the extract given in a therapeutic dose.