13C - DEPT - Proc - (Chinese + English)

¹³C + DEPT數據處理-Proc (中文版)

1. re (13C 檔名) ( expno 實驗區 )

lb 1 ~ 3

efp

apk

abs n

利用solvent peak 定標

Peak picking

2. re (dept90檔名) ( expno 實驗區 )

lb 1 ~ 3,

efp

phasing( 將所有峰之相位調成正相) or apk

使用已知之化學位移(從 13C譜)定標(sr)

3. re (dept135檔名) ( expno 實驗區 )

lb 1 ~ 3, ef

將dept90 之相位phc0, phc1 代入Procpars 的 Phase

Correction

(或將1級碳及3級相位調成正, 2級碳相位調成負 )

使用已知之化學位移(從 13C譜)定標

4. re (13C 檔名) ( expno 實驗區 )

鍵入 edc2 , 出現 2 欄多列之表格

(左邊 dataset2 = [dept90] : filename, expno, procno )

(右邊 dataset3 = [dept135]: filename, expno, procno)

¹³C + DEPT - Proc (English Version)

1. re (expno of # 13 C )

efp, phase(use phc0, phc1) or apk

calibration reference (use solvent peak )

check sr

abs n automatic baseline correction without integral (do b4

peakpicking)

peak picking ; define Xmin , Xmax( f1p , f2p)

2. re (expno of # dept90 )

efp, phase ( phase all the peaks as positive)

calibrate the chemical shift or type in sr of 13C

3. re (expno of # dept135 )

efp, phase

type in the sr to calibrate the chemical shift

4. edc2 (type this command in expno of 13C)

Dataset 2 : dept90 (filename, expno, procno) , procno = 1 (left side of table)

Dataset 3 : dept135 (filename, expno, procno) , procno = 1 (right side of table)

DEPT experiment and Phasing

For DEPT experiment, peak phase with upside is positive.

Peak phase with downward is negative.

Experiment

For DEPT45, it yield CH, CH2 and CH3 with positive phase.

For DEPT90 ,only yield CH and it was phase up.

For DEPT135, CH, CH3 are phase up and CH2 is down.

Plotting

Finally, you have to check out that all phase in (DEPT90, DEPT135) should be in consistence.

Adjust the intensity of the peaks in each experiment(slice) so that it results in same height for the same peaks .