HMBC 

Two-dimensional Heteronuclear Multiple Bond Correlation Spectroscopy 

HMBC - ACQU

1. Insert the sample, lock the field, shim, and perform tuning and matching (atmm) for all relevant nuclei (carbon and hydrogen).

2. Measure the 90-degree pulse width for 1H.

3. Measure the 1D 1H spectrum -> For plotting, use it as a side plot for F2 projection (use 13C spectrum experiment for F1 projection).

4. Use either rpar bbfo_13C_hmbc or rpar bbfo_c13hmbcet (choose one to load) all.

5. getprosol for 1H (90pw) (pl).

6. Run rga.

7. Set ns to 16-64 (depending on concentration).

8. expt

9. Acquire zg.

10. Run the experiment again to check the required time(expt).

HMBC - PROC

1. Execute Fourier transform of both dimensions (xfb).

2. If there are no negative peaks, skip this step (xf2m: magnitude mode).

3. Calibration (choose an independent signal for calibration; correct F2 domain with 1H chemical shift; correct F1 domain with 13C chemical shift).

4. Perform baseline correction of both dimensions: abs2, abs1.

5. Enter edc2:

   • Data Set2 = (filename of 1H)

   • Data Set3 = (filename of 13C)

6. Select the printer icon, Layout: +2D_CH_ntu.xwp

Note: HMBCET has positive and negative phase differences; perform xfb first, then xf2m.

二維碳氫異核長距離化學位移相關光譜

HMBC – ACQU

1. 置入樣品，鎖場、勻場及 atmm  所有相關核種(碳及氫)

2. 量測1H之90度脈寬

3. 量測一維之1H譜 -> 製圖時，當側圖使用- for F2 projection (另外F1 projection 請用1 3C譜實驗)

4.  rpar bbfo_13C_hmbc

  或rpar bbfo_c13hmbcet (擇一讀入) all

5.  getprosol 1h (90pw) (pl)

6. rga

7. ns  16~64  (視濃度而定)

8. expt

9. zg

10. expt    (檢查實驗所需之時間)

HMBC - PROC

1.     xfb  (execute fourier transform of both dimensions)

2.     xf2m   (若沒有負峰, 跳過此步 ,  m : magnitude mode)

3.     calibration ( 定標：擇一獨立訊號， F2 domain 以1H的化學位移訂正；F1 domain 以13C的化學位移訂正)

4.    abs2, abs1  (baseline correction of both dimension :  2個維度的基線調整) 

5.    edc2  

        Data Set2 = ( filename of  1H  )

        Data Set3 = ( filename of  13C)

6.     選擇printer icon ，Layout：+2D_CH_ntu.xwp

註：HMBCET 有正負相位之別，xfb 後再 xf2m