Tocsy 

2D - TOCSY – ACQU

(Two-dimensional Proton-Proton Total Correlation Spectroscopy)

1. Insert the sample, lock the field, shim, and perform tuning and matching (atmm) for hydrogen nuclei.

2. Measure the 90-degree pulse width for 1H.

3. Measure the 1D 1H spectrum -> For plotting, use it as a side plot for F2/F1 projection.

4. Use rpar bbfo_tocsy all.

5. Retrieve processing parameters (getprosol or gpro).

6. Set the 90-degree pulse width of 1H as p1.

7. Reduce receiver gain (rg) slightly.

8. Set ns to 4-16.

9. Set 1 td = 512.

10. Acquire zg (get a 2D spectrum).

Note: Data from TOCSY experiments may require phase adjustment and noise reduction processing as necessary.

Note: If the water peak or solvent peak in the sample is too strong, it may need to be addressed separately.

Note: expt can be used to check the time required for the experiment.

2D - TOCSY – ACQU

二維氫氫同核全化學位移相關光譜

1. 置入樣品，鎖場、勻場及atmm氫核

2. 量測1H之90度脈寬

3. 量測一維之1H譜 -> 製圖時，當側圖使用- for F2 /F1 projection

4.  rpar bbfo_tocsy all

5.  getprosol (gpro)

6. 將1H 之 90 度脈寬代入p1,

7.  rg 改小一點

8.  ns   4 ~16

9.  1 td =512

10. zg

註：Tocsy 之數據需要調整相位及視情況作噪音去除處理

註：樣品中之H2O 峰或溶劑峰若太強需要另外處置

註：expt 可檢查實驗所需之時間