Mix Solvents 及多種 D 之鎖定方法

Mix Solvents and Locking Procedure for Multiple Solvents:

1. Type "lock".

   • Select the solvent for the sample from the solvent menu, e.g., CD3OD.

2. Once the lock is automatically engaged (lock light is steady), type  <rga; zg; fp>.

3. Check if the chemical shifts on the spectrum are correct.

4. If the chemical shifts are incorrect, release the lock (unlock).

5. Move the signal that needs to be locked toward one side of the lock window, ensuring that the signal to be locked is closest to the center of the lock window as possible.

   • (Refer to the chemical shift data in the "edlock" for guidance.)

   • Since the center of the lock window is where the field is locked (adjustable by pressing the field keys to move the lock signal field).

6. Press the "lock" button on the bsmsdisp window.

7. For example, when using CD3OD as the solvent, the instrument's lock database locks onto the CD3 signal.

   • Scenario 1: If CD3OD automatically locks onto CD3, the chemical shifts on the spectrum should be correct, and the experiment can proceed.

Scenario 2: If CD3OD automatically locks onto OD instead of CD3, it is necessary to release the lock to prevent incorrect chemical shifts from affecting the detection range. 

At this point, use the field keys to move both D signals (since this solvent has two deuterium signals) to the extreme left side of the lock window (both signals should cross the centerline of the lock window). Because the field of CD3 is higher than that of OD, this will make CD3 closer to the center distance of the lock window than OD. When you press the lock button again in the bsmsdisp window, the instrument is more likely to lock onto CD3. 

Note:

• _____  Indicates pressing the BSMS button.

• <        > Indicates keyboard input commands.