Exam Instructions:

1. All users must pass the exam to obtain operation and access permissions before they can operate independently. They are not allowed to operate the equipment alone before passing the exam.

2. Those wishing to obtain operation permission must thoroughly understand the operating methods and procedures and have practiced on the machine at least five times (with supervision by a manager or a certified person).

3. Practice sessions are generally 30 minutes each (scheduled by the manager or a permitted operator).

Eligible Users for Training and Exams:

1. Researchers in this department, with the consent of their advisor.

2. Only users related to this department.

3. During the summer, NMR training is mandatory, and passing the exam grants an NMR user license.

Exam Principles:

1. The manager is responsible for preparing test samples, typically known samples.

2. The examinee must complete the data collection and processing of proton and carbon spectra (including DEPT series experiments) within the specified time and in the presence of the manager.

3. The manager may suspend the exam depending on the situation. Those who fail the exam must practice on the machine twice more before reapplying for the exam.

4. If incorrect operation during the exam causes equipment damage, the repair costs will be shared by the examinee's affiliated laboratory.

Exam Location:

A525 Bruker AVIII HD 400NMR (Topspin 3.5 pl7)

Exam Application:

Examinees should schedule their exam time with the manager.

Please send an email to hsiching@g.ntu.edu.tw (Dr. Hsi-Ching) for arrangement.

(Please include your student ID, name, dates of practice, and laboratory (advisor)).

(Inform the manager of several convenient dates and times for the exam, or inconvenient times).

Exam Content (Practical + Written Test + Signing the NMR Instrument Room Usage Rules):

Practical Exam:

• There is a time limit for the practical exam (20 minutes for 1H + 13C + DEPT).

• Manuals, notes, or cheat sheets are not allowed.

Additional Written Test:

• The content includes:

  1. Usage rules of the rota; including personal and laboratory rules.

  2. Precautions.

  3. Basic commands.

  4. Violations.

• The written test lasts 2 - 3 minutes, and manuals are not allowed.

• Note: The prohibition of notes or cheat sheets during the exam ensures that examinees have indeed practiced on the machine.

Spectra Processing:

• Spectra should be processed in your own laboratory (no time limit).

• When submitting the spectra, it is also the time to apply for access settings.

  3. Create a PDF file for the 1H spectrum.

  4. Create a single PDF file for 13C + DEPT90 + DEPT135 spectra.

  5. Note: The PDF must include the parameter section (time and date of the exam).

  6. Email the PDF file to hsiching@g.ntu.edu.tw (Dr. Hsi Ching).

  7. Submit the paper access application form to A116 NMR Lab.

The interval between the first and second exam applications must be 4 weeks.

Please practice extensively and observe how others use the instrument before applying for the exam.

Access Application:

1. The application form will be provided on the day of the exam. After emailing the spectra, submit the access application form to A116.

2. If the access application has not taken effect within two working days, please email your name and student ID to notify.

 考試說明：

 1.　所有使用者均需通過考試取得操作與門禁許可方可獨立操作，在未通過考試前不得獨自操作儀器。

2.　欲取得操作許可者在參加考試前需確實了解操作方法與步驟，且上機實際練習五次以上

         (需有管理人員或已取得認證者在現場指導) 。

3.　練習時間每人每次以 30 分鐘 (由管理人員或有操作許可者預約實驗室時段) 為原則。

可參與訓練及考試之使用人員：

1.本系從事相關研究者，經指導教授同意者 

2.僅限本系相關使用者

3.暑假時一定需要上 NMR 教育訓練 ，經過考試可取得 NMR 使用執照

考試原則：

1.管理人員負責準備測試樣品，原則上以已知樣品為主。

2.考生須於指定時間內，於管理人員在場時，自行完成氫譜與碳譜(含 DEPT 系列實驗)數據之採集與處理。

3.管理人員得視情況中止考試，未通過考試者需再上機練習始得申請參加考試。

4.考試過程中若不正確之操作而導致設備損壞時，由該員所屬之實驗室共同分擔維修費用。

考試地點：

A525 Bruker AVIII HD 400NMR (Topspin 3.5 pl7)

考試報名：

請查看臺大化學系公告網頁

 考試內容(操作 + 筆試 + 簽NMR儀器室使用守則)：

操作考試有時間限制 (20 mins  = 1H + 13C + DEPT)， 不可以看 Manual、筆記或小抄

另外會加考筆試：

內容含 

　(1)輪簽之使用規則;包括個人及實驗室

      (2)注意事項

      (3)簡易指令

      (4)違規行為

(筆試：2 ~ 3 分鐘，不可以看 Manual)

(備註：考試時不能看筆記或小抄是為了

落實考照之同學確實上機練習)

圖譜處理：

圖譜回自己實驗室處理 (沒有時間限制)， 

繳交圖譜的時候，即是申請門禁設定之時間

(1) 1H 譜劃一張 ->作成 pdf 檔

(2) 13C + DEPT90 + DEPT135 -> 劃成一張圖譜(作成 pdf 檔)

註：pdf 檔內務必含參數欄 parameter - 即考試時間 + 日期

(3) 圖譜 pdf 檔 Email 至 hsiching@g.ntu.edu.tw (曾喜青博士 )

(4) 門禁申請單紙本 -> 繳交 A116 NMR Lab

第一次申請考試與第二次申請考試須相隔 4 星期

請多加練習並多觀察別人如何使用再來申請考試

門禁申請：

(1) 申請單會在考試當天提供，圖譜 Mail 出後再將門禁申請單繳交至 A116

(2) 若申請門禁超過 2 個工作天仍然不生效，請 Email 告知姓名及學號