H-H COSY (二維同核相關光譜)

(Homonuclear COrrelation SpectroscopY)

1H - ACQU

1.    Place the sample with a height of 4.0 cm into the spinner, wipe the lower part of the glass sample tube with a paper towel, and measure the 

        depth where the sample should be placed using a gauge (BBFO = 2.0 cm).

2.      Press Lift to send air to the top of the Magnet, then slowly insert the sample attached to the spinner into the probe inside the Magnet.

3.      edc Enter (filename), (expno)

4.      rpar bbfo_1h

5.      getprosol  

6.      lock (Lock according to the solvent being used)

7.      atmm (tuning and matching - manually)

8.      topshim (shimming) 

9.      Further adjust shimming in the x, y

10.    rga (receiver gain adjust)

11.    ns 16

12.    zg (get a 1H spectrum for use as a side plot in two-dimensional experiments )

COSY - ACQU

13.   edc (edit the other expno)

14.   rpar bbfo_1h_cosy or rpar bbfo_cosy

15.   getprosol 

16.   p0 = (p1 / 2), (p1 = 90 pulse width, p0 = 45 pulse width)

17.   ns 4

18.   rga

19.   zg

20.   expt (experiment time)

COSY - PROC

21.   xfb (Execute the two-dimensional Fourier transformation)

22.   sym (Perform symmetry correction.Both dimensions represent the same type of nucleus.)

23.   abs2D (Conduct baseline correction in both dimensions: abs2, abs1)

24.   calibration (F1 domain and F2 domain)

   (identifying specific chemical shifts, such as those of solvent peaks, along the diagonal and using them as reference points.)

25.   nlev (24  ~ 48) (number of contour levels ), levcalc,

        edlev (edit level), Choose positive and level increment to values such as 1.3 to 2.2. 

        Then, press "Fill" and "Apply" 

安置側圖法 (Projection placement)

Method (一) edc2 

(Fill in the table with 1H file-related information, such as expno, procno, hard disk, directory, etc.)

Use "rep 1" to repeat the process. (re procno 1 can update the screen).

Method (二)   

Move the cursor to the frame above the 2D Projection.

Right-click (first button), choose External Projection.

Fill in the 1H file-related information, such as Name, expno, procno, directory.

Move the cursor to the frame on the left side of the 2D Projection and proceed accordingly.

COSY - PLOT

Select "PLOT"

Choose "Layout".

Select "Export" from Menu 3, then save as .pdf.

Note: Commands are indicated in blue, while actions are indicated in red.

H-H COSY (二維同核相關光譜)

(Homonuclear COrrelation SpectroscopY)

1H - ACQU

1.      將 4.0 cm 高溶液的樣品套上轉子 (spinner) 用紙巾擦拭玻璃樣品 

          管下半部, 用 gauge 量好樣品應置放之深度 (BBFO = 2.0 cm)

2.      按 Lift 將空氣送達Magnet上方中，將套好轉子的樣品緩緩送入

         Magnet 內的探頭中

3.      edc 鍵入 (filename), (expno)

4.      rpar bbfo_1h

5.      getprosol  

6.      lock (依據使用的溶劑鎖場)

7.      atmm (tuning and matching - 手動調諧)

8.      topshim (勻場) 

9.      再加 shim x, y

10.    rga (receiver gain adjust)

11.    ns 16 

12.    zg (get a 1H spectrum - 當二維之側圖用)

COSY- ACQU

13.   edc (edit the other expno)

14.   rpar bbfo_1h_cosy 或 rpar bbfo_cosy

15.   getprosol 

16.   p0 = (p1 / 2), (p1 = 90 pulse width , p0  = 45 pulse width)

17.   ns 4

18.   rga

19.   zg

20.   expt (實驗時間)

COSY - PROC

21.   xfb ( 執行二維的傅立葉轉換)

22.   sym ( 進行對稱化, 二維都是相同核種)

23.   abs2D (2 個維度之基線調整: abs2, abs1)

24.   calibration (F1 domain 及 F2 domain)

   (於對角線上找出特定化學位移例如溶劑加以標定)

25.   nlev (24  ~ 48) (contour level 圈數), levcalc,

        edlev (edit level), 選擇 positive, level increment 可設 1.3 ~ 2.2

        再按 Fill, Apply

安置側圖法  (Projection placement )

方法 (一) edc2 

(表格左右欄都填入 1H 檔案相關訊息，如 expno, procno, hard disk, directory 等)

   rep 1 (re procno  1，可將螢幕更新)

方法 (二) 將游標移到 2D 上方的 Projection 的框架內，按下右鍵（第一鍵），選擇External Projection， 

         填入 1H 檔案相關訊息，如 Name, expno, procno, directory，將游標移到 2D 左方的 Projection 的框架內，

         依法泡製

COSY - PLOT

    挑選 PLOT

    挑選 Layout

    挑選 Menu 3 的 Export，存成 .pdf

註: 藍色字表指令 紅色字表按鍵