TRPV1 expression during embrionics stem cell neuronal differentiation

Embrionic Stem (ES) cells are considered as new strategy to replace damage or death cells in many neurodegenerative disorders like Parkinson disease. The efficiency of ES graft depends on their ability to survey after the grafting and their capacity to connect with the central network in brain. New strategies are now developing to achieve this critical aspect. In particular the utilization of TRPV1, a non selective cation channel activated by temperature, voltage and pH in others, as been assessed to modulate the activity of neurons in central nervous system remotely and to induce protection against cell death. However no evidence exists to utilize this channel to control the excitability of neuronal precursor or the cell resistance against cell death. We expect to control the activity of ES in grafting and also improve the cell survival by the modulation of TRPV1. In this context we purpose as first objective to characterize the expression of TRPV1 in different stage of differentiation from ES cells to neurons. We found expression of TRPV1 in both ES and Neuronal stem cells but not in stem cells-derived neurons or glia. We need to confirm these results by functional assay and develop a new strategy to control de activity of stem cells derived-neurons or neuronal precursor by TRPV1 in vitro.

Ecos-Conicyt - Biomarkers and Cell Replacement Therapy at Early Parkinson’s Disease: From Human Electrophysiology to Cell Grafting in a Non-Human Primate Model

Escuela de Medicina, Universidad de Valparaíso, Hontanteda 2664, Valparaíso, Chile.

Inserm, U846, Stem Cell and Brain Research Institute, France.