CRISPR-Cas9

Bacteria and genes mutation with CRISPR-Cas9

I made a genome mutation (K43T) from a Lysine (K) to a Threonine (T) in E.coli colonies.

Results after CRISPR-Cas 9: The colonies can no longer communicate with each other and therefore can no longer "grow". The colonies become independent colonies from each other. In addition the bacteria will "survive on Strep media which would normal prevent its growth".

Before CRISPR Cas-9 manipulation

Colonies of non-pathogenic E.coli bacteria (DH5a) with simple Agar plate.

After CRISPR Cas-9 manipulation

Agar plate made with Strep/Kan/Arabinose.

Jellyfish gene into bacteria

DNA modification and insert Green Fluorescent Protein from a Jellyfish gene inside a bacteria E.Coli (non-pathogen). I used plasmid and not CRISPR-Cas9 in this case. Transformed colonies are become ampicillin resistant.

Insert Green Fluorescent Protein from a Jellyfish gene inside a bacteria (non-pathogen E.Coli), and see how electrons are excited by ultraviolet light and how they absorb photons.

DNA modification (bacteria thinks that fluorescent protein is from his own DNA...) under ultraviolet light.

Colony of E.Coli DH5a (non-pathogen) before DNA modification.

Colony of E.Coli DH5a (non-pathogen) after DNA modification.

Colony of E.Coli DH5a (non-pathogen) again, because why not?

E.Coli DH5a, plasmid, LB Agar, etc. Everything you need to make some DNA modification in a Petri dish.

Experience preparation.

Me in action.

Bacteria and microbials organisms samples

Some Petri plates with different bacteria and microbials organisms. My goal is to work with human tissue culture in few months.

The images and the website are copyrighted and not free of rights.