Web Documentation

Sequence Submission

The sequence submission form can be found at https://vac.kamalrawal.in/vaxelan/v2. One or more protein (up to 60) sequences in FASTA format or one UniProt ID can be pasted into the text box, or the "Browse..." option can be used to analyze a file of one or more (up to 60) sequences in FASTA format stored in the user's computer. Also, choose the appropriate input format based on your input. Optionally, you can provide your email address to receive notification of the result link.

Choose appropriate threshold settings

1. Literature-based cutoffs (Default)

Cutoffs were found out using a literature search. Click here to see literature-based cutoffs.

2. Optimized cutoffs

Cutoffs were found out using ROC curves. Click here to see optimized cutoffs and methodology.

Choose a predefined strategy method (Optional)

1. Strategy 1

In this strategy, we use 7 filters were incorporated to screen the proteins. These include subcellular localization, subcellular localization score, non-homology, stability analysis, allergenicity, antigenicity, and adhesion property. Antigenicity does not include in the web version.

2. Strategy 2

In this strategy, we use 7 filters were incorporated to screen the proteins. These include non-homology, stability analysis, allergenicity, antigenicity, adhesion property, and ic50. Antigenicity does not include in the web version.

3. Strategy 3

It included the random filtering of proteins (not in a sequential manner). You can use the 'Order of filter (draggable)' component to randomly order the filters present in that component.

4. Strategy 4

We use all possible tools (without elimination/filtering) to perform a comprehensive evaluation of query protein sequences/proteome.

Select parameters

Check the checkbox assigned to the parameter to include it in the analysis. If you have selected a predefined strategy then you may found out that some parameters were disabled.

Change filtering order (Optional)

If you have selected the option 'Apply Filter Order' then you can change the order of selected filters by just dragging them.

Waiting for Results

There are two ways to receive results from VaxElan i.e. via email (contains result link) or wait for the results page (wait till analysis is completed and then automatically the user is redirected to the result link or you can bookmark this page to check later).

After a successful analysis of the input, the result link will be provided to the user via email if provided. In case, the server is unable to parse the user's input or the server encounters an exception during analysis, an explanation for the same will be sent to the user via email or you can check the earlier bookmarked page for an explanation.