Numerous studies have suggested that epitope-based antigens can induce protective immunity against different infectious agents. Various methods have been described in the literature to determine the B and T-cell epitopes which include; functional assays wherein the antigen is sometimes mutated and antibody-antigen interaction is evaluated, 3D structure analysis of antigen-antibody complexes or screening the peptide library of antibody binding, utilization of MHC multimers, and lymphoproliferation by ELISPOT assays (. Apart from these time consuming and expensive experimental techniques, scores of computational methods have also been developed in the past few years. In the subsequent section, we shall describe different approaches for prediction of B-cell, MHC-I, and MHC-II epitopes in potential vaccine candidates
In this study, we have utilized multiple tools for identifying and selecting through a rigorous approach which will be explained in the sub-sections.