Comparative analysis of Strategies
With the aim of identifying unique and common epitopes across all the strategies, comparisons were made.
With the aim of identifying unique and common epitopes across all the strategies, comparisons were made.
Table displaying the logic and result achieved by strategies:
Table displaying the logic and result achieved by strategies:
Strategy 1 vs 2:
Strategy 1 vs 2:
From this comparison, some protein were found to be retained and rose to the top of shortlisted candidates. It includes XP_809166.1 mucin-associated surface protein (MASP) & XP_818708.1 trans-sialidase .
From this comparison, some protein were found to be retained and rose to the top of shortlisted candidates. It includes XP_809166.1 mucin-associated surface protein (MASP) & XP_818708.1 trans-sialidase .
Strategy 1 vs 3:
Strategy 1 vs 3:
As mentioned above, Strategy 1 and Strategy 3 comprises of same filters, they have resulted in to same shortlisted proteins.
As mentioned above, Strategy 1 and Strategy 3 comprises of same filters, they have resulted in to same shortlisted proteins.
Strategy 1 vs 4:
Strategy 1 vs 4:
With the binarization of protein features, the top ranking feature candidates were compared and we have not found any similar protein.
With the binarization of protein features, the top ranking feature candidates were compared and we have not found any similar protein.
Strategy 1 vs 5:
Strategy 1 vs 5:
No proteins were identified as common protein vaccine candidate.
No proteins were identified as common protein vaccine candidate.
Following Image depicts top 8 proteins in each of the strategies:
Following Image depicts top 8 proteins in each of the strategies:
It denotes Strategy 3 has no new proteins. On the other hand, XP_818708.1 trans-sialidase & XP_816522.1 (Mucin TcMUCII) were found to be conserved in Strategy 1, 3 & 5.
It denotes Strategy 3 has no new proteins. On the other hand, XP_818708.1 trans-sialidase & XP_816522.1 (Mucin TcMUCII) were found to be conserved in Strategy 1, 3 & 5.
The attached excel file shows top 100 proteins for each of the strategies in an excel format:
The attached excel file shows top 100 proteins for each of the strategies in an excel format:
Comparitive_work_strategies.xlsx