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7 filters were incorporated to screen the proteins of T.cruzi CL Brener.
7 filters were incorporated to screen the proteins of T.cruzi CL Brener.
With the aim being construction of a novel vaccine against Chagas disease, we used deep learning approaches for filtering the superset of protein sequences. The proteins were shortlisted on the different parameters such as shown in the figure. A series of mentioned filters resulted in 100 proteins of concern from a raw set of 19,602 proteins. Among these 100 proteins, 8 proteins with highest antigenic scores from each unique family were selected to be potential candidates for vaccine designing. Furthermore, the epitopes were predicted from these 8 proteins.
With the aim being construction of a novel vaccine against Chagas disease, we used deep learning approaches for filtering the superset of protein sequences. The proteins were shortlisted on the different parameters such as shown in the figure. A series of mentioned filters resulted in 100 proteins of concern from a raw set of 19,602 proteins. Among these 100 proteins, 8 proteins with highest antigenic scores from each unique family were selected to be potential candidates for vaccine designing. Furthermore, the epitopes were predicted from these 8 proteins.
Flowchart representing the filters in a sequential order with number of proteins filtered mentioned in brackets.
Flowchart representing the filters in a sequential order with number of proteins filtered mentioned in brackets.
The files in sequence of data obtained, after each filter was run on the 19,602 proteins.
The files in sequence of data obtained, after each filter was run on the 19,602 proteins.
Following file contains python script which filters the data, and their successive output at each level of filtering. Filter 1 denotes "Sub cellular Localization", filter 2 denotes "Sub cellular Localization score". Additionally, filter 7 comprises of the final file which is the output of the filtering process.
Following file contains python script which filters the data, and their successive output at each level of filtering. Filter 1 denotes "Sub cellular Localization", filter 2 denotes "Sub cellular Localization score". Additionally, filter 7 comprises of the final file which is the output of the filtering process.
Top 8 unique proteins:-
Top 8 unique proteins:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_813527.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_809835.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-1 vs Strategy-2
Strategy-1 vs Strategy-2
Intersection of proteins in Strategy-1 and Strategy-2:-
Intersection of proteins in Strategy-1 and Strategy-2:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
Union of proteins in Strategy-1 and Strategy-2:-
Union of proteins in Strategy-1 and Strategy-2:-
Strategy-1:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-2:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_811700.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_822020.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_804447.1 ubiquitin-conjugating enzyme E2 [Trypanosoma cruzi strain CL Brener]
XP_807999.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_806523.1 IgE-dependent histamine-releasing factor [Trypanosoma cruzi strain CL Brener]
XP_808372.1 small nuclear ribonucleoprotein Sm-E [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
This implies that Strategy 1 and Strategy 2 which differs from each other through the sub-cellular localization filters has retained some protein candidates among them.
Strategy-1 vs Strategy-3
Strategy-1 vs Strategy-3
Intersection of proteins in Strategy-1 and Strategy-3
Intersection of proteins in Strategy-1 and Strategy-3
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Union of proteins in Strategy-1 and Strategy-3:-
Union of proteins in Strategy-1 and Strategy-3:-
Strategy-1:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-3:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-1 vs Strategy-4
Strategy-1 vs Strategy-4
No intersection of proteins were found upon filtering of proteins via Strategy-1 and Strategy-4.
Union of proteins in Strategy-1 and Strategy-4:-
Union of proteins in Strategy-1 and Strategy-4:-
Strategy-1:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-4:-
XP_816052.1 phosphate-repressible phosphate permease [Trypanosoma cruzi strain CL Brener]
XP_807493.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_803296.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_822017.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_819804.1 nucleoside transporter-like [Trypanosoma cruzi strain CL Brener]
XP_820852.1 serine/threonine protein kinase [Trypanosoma cruzi strain CL Brener]
XP_815460.1 receptor-type adenylate cyclase, partial [Trypanosoma cruzi strain CL Brener]
XP_812509.1 UDP-N-acetylglucosamine--dolichyl-phosphate n- acetylglucosaminephosphotransferase [Trypanosoma cruzi CL Brener]
This implies that most of the protein candidates identified in Strategy 4 don't match the features of proteins identified via strategy1
Strategy-1 vs Strategy-5
Strategy-1 vs Strategy-5
No intersection of proteins were found upon filtering of proteins via Strategy-1 and Strategy-5.
Union of proteins in Strategy-1 and Strategy-5:-
Union of proteins in Strategy-1 and Strategy-5:-
Strategy-1:-
XP_809166.1 mucin-associated surface protein (MASP) [Trypanosoma cruzi strain CL Brener]
XP_816522.1 mucin TcMUCII [Trypanosoma cruzi strain CL Brener]
XP_815016.1 90 kDa surface protein [Trypanosoma cruzi strain CL Brener]
XP_818708.1 trans-sialidase [Trypanosoma cruzi strain CL Brener]
XP_821916.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_804513.1 dispersed gene family protein 1 (DGF-1), partial [Trypanosoma cruzi strain CL Brener]
XP_816111.1 subtilisin-like serine peptidase [Trypanosoma cruzi strain CL Brener]
XP_806816.1 DnaJ chaperone protein [Trypanosoma cruzi strain CL Brener]
Strategy-5:-
XP_809117.1 hypothetical protein [Trypanosoma cruzi strain CL Brener]
XP_821990.1 mucin-associated surface protein (MASP [Trypanosoma cruzi strain CL Brener]
RNC42421.1 mucin TcMUCII [Trypanosoma cruzi]
XP_803883.1 GP85-like protein, partial [Trypanosoma cruzi strain CL Brener]
AAL90889.1 Tcc1j12.3 [Trypanosoma cruzi]
XP_809960.1 trans-sialidase [Trypanosoma cruzi strain CL brener]
XP_808931.1 mucin TcMUCIII {Trypanosoma cruzi strain CL Brener]
Strategy 5 was of ORF predictions and it implies that there was no same protein candidates which arises to top from both strategies.
Top 100 proteins retrieved by Strategy-1
Top 100 proteins retrieved by Strategy-1
drive.google.com/file/d/1ozMnT3zvc2zYR7TUVXgetEtLLu01psGF/view?usp=sharing
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