Variability Sequence Analysis of T. Cruzi epitope

Retrieval of sequences

Protein and genomic sequences of trans-sialidase were retrieved from NCBI and TriTrypDB. Epitope sequence (SSDADPTVV) of XP_818708.1 (Trans-sialidase) was predicted using RV tools.

Total five sequence of trans-sialidase belongs to different strains such as Trypanosoma cruzi Dm28c 2017, Trypanosoma cruzi Sylvio X10/1-2012, Trypanosoma cruzi marinkellei strain B7, Trypanosoma cruzi Dm28c 2014, and Trypanosoma cruzi CL Brener Non-Esmeraldo-like were retrieved from TriTrypDB database using keywords “trans-sialidase AND Trypanosoma cruzi”. Details are given in table 1.

Sequence analysis

Bioedit software 7.2.5 (Hall et al., 1999) was used to perform several analyses such as sequence alignment, Translate, Reverse-translate, reverse complement of epitopes with protein and genomic sequences.

To check the similarity of sequences, analysis was performed in following categories which is described below.

1. Query protein alignment with epitope sequence

2. Translated genomic sequence alignment with epitopes sequence

3. Translated genomic sequence alignment with query protein sequence

4. Translated epitope sequence alignment with genomic sequence

5. Alignment of reverse complement sequence of epitope with genomic sequence

6. Alignment of translated genomic sequence of strains with query protein sequence