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Roberto Sitia Lab
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Roberto Sitia
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Lab Members
Anelli Tiziana
Marco Dalla Torre
Chiara Giannone
Andrea Orsi
Cecilia Palazzo
Tiziana Tempio
Ilaria Sorrentino
Elisa Speranza
Past members
Maria Francesca Mossuto
Sannino Sara
Fagioli Claudio
Stefano Bestetti
Bertolotti Milena
Iria Medraño Fernandez
Research
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Protocols
Biochemistry
AMS
AMS protocol
Antibodies purification from serum or ascite
ATP
BCA
Common Solutions
Coupling to CNBr-Sepharose
Crosslinking Ab to PGS / PGA
DSP crosslinking
ECL homemade
ELISA for IgM or IgG
Glycosidase Digestions
MOPS
mPEG protocols
PFA 4%
Precipitating Proteins
Precipitating Proteins
Protease Protection Assay
Protease protection assay
SDS lysis for Ribophorin
SDS PAGE GRADIENT
SDS-PAGE
Stripping solution (NaOH)
Triton X114 separation
Trypsin digestion
Immunoprecipitation
Molecular Biology
Competent cells
DNA precipitation
LB Agar
Medusa Protocol
MiniPrep
Quick Transformation
quicke extraction from bacteria
RNA extraction Tryzol
Top10 competent cells
Transformation
Cell Biology
Annexin V - PI Staining
Haemolysis assay
IF (suspension cells)
Intracellular staining for FACS
J chain folding assay
JetPEI transfection
Lentiviral Packaging
MPA Medium
Mycoplasma detection by PCR
Mycoplasma test from Blasi lab
NSO J558L electroporation
PEI
Reoxidation and trypsin digestion IP3R
Subcloning
Xbp1 splicing (EvA lab)
Myc detection PCR
ImageJ tips
Immunology
aCD3-stimulation
Atg5 PCR
B cell purification
CFSE
Intracellular Staining
NP immunization and serum ELISA
NP-ELISA
tail DNA extraction
Cakes
Crostata di marmellata
Linzer Torte Originale
Materials generated by us
ERp44-/- CRISPR-KO HeLa cells
EvA Cells
Manuals & Datasheets
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Ordini (new version)
Ordini (old version)
Ordini - Chiamate 5656 - Ott2016
Antibodies Lab
Antibodies Andrea Orsi
Chemicals
Fridges and Freezers
Liquid Nitrogen
Column A
A2
A3
Column B
Column C
Column G
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Plasmids
Roberto Sitia Lab
Plasmid Database
Plasmid Database RS+EvA_UP TO DATE!.xlsx
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Plasmid Database RS+EvA_UP TO DATE!.xlsx