Immunoprecipitation
Lysate should not contain more than 0.1% SDS, in case diluite with PBS.
Pre-clearing from 1/2h to O/N at 4°C on rotation by adding 15µL of beads sepharose-FCS (30µL of 1:1 seph beads in PBS)
Pellet beads 1' 9000xg
Transfer lysate supernatant to new eppendorf
Add 15µL of dry beads with antibody either crosslinked or by adding 1µg of Ab
On rotation o/n 4°C
Pellet beads 1' 6000rpm
Wash 3 times: 2x STN (solution; 1x H2O wash (10mM Tris pH=7,4)