Immunoprecipitation

Lysate should not contain more than 0.1% SDS, in case diluite with PBS.

Pre-clearing from 1/2h to O/N at 4°C on rotation by adding 15µL of beads sepharose-FCS (30µL of 1:1 seph beads in PBS)

Pellet beads 1' 9000xg

Transfer lysate supernatant to new eppendorf

Add 15µL of dry beads with antibody either crosslinked or by adding 1µg of Ab

On rotation o/n 4°C

Pellet beads 1' 6000rpm

Wash 3 times: 2x STN (solution; 1x H2O wash (10mM Tris pH=7,4)