ImageJ tips

Copy a selection in multiple images. Click on the image with the selection. Click on the image to which you want to copy the selection. Type SHIFT-e. (or alternatively use the synchronize windows ImageJ plugin)

Quantifying multiple bands:

• align bands carefully

• draw rectangle around bands

• press cmd + 1 (select first lane)

• press cmd + 3 (plot bands)

• measure area below peaks with magic wand

Check if an image is saturated:

• Image -> Lookup tables -> HiLo