SDS-PAGE

LOWER GEL:(40ml)

10 ml lower buffer 4X

400 ml APS 10%

20 ml TEMED

7% 8% 10% 12% 14% 15%

UPPER GEL: ( 10ml)

2.5 ml upper buffer 4X ( 0,5M Tris-HCl pH=6,8 0,4%SDS)

6.4 ml H2O

1 ml Acrilamide bis 30%

200 ml APS

10 ml TEMED

RUNNING BUFFER

100 ml Tris-Gly 10X

900 ml H₂O

5 ml SDS 20% ( O.1% finale)