Atg5 PCR

2 µL of tail purified DNA in (resuspended in 100µL)

Primers (0.5µL of 10µM in 25µL reaction)

ATG5 FW

ATG5 RW

Program:

25 cycles of

30'' 95°C

30'' 51°C

30'' 72°C

(begin with 5' 95°C and end with 5' 72°C)