2 µL of tail purified DNA in (resuspended in 100µL)
Primers (0.5µL of 10µM in 25µL reaction)
ATG5 FW
ATG5 RW
Program:
25 cycles of
30'' 95°C
30'' 51°C
30'' 72°C
(begin with 5' 95°C and end with 5' 72°C)