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Roberto Sitia Lab
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Anelli Tiziana
Marco Dalla Torre
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Maria Francesca Mossuto
Sannino Sara
Fagioli Claudio
Stefano Bestetti
Bertolotti Milena
Iria Medraño Fernandez
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AMS protocol
Antibodies purification from serum or ascite
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Coupling to CNBr-Sepharose
Crosslinking Ab to PGS / PGA
DSP crosslinking
ECL homemade
ELISA for IgM or IgG
Glycosidase Digestions
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mPEG protocols
PFA 4%
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Precipitating Proteins
Protease Protection Assay
Protease protection assay
SDS lysis for Ribophorin
SDS PAGE GRADIENT
SDS-PAGE
Stripping solution (NaOH)
Triton X114 separation
Trypsin digestion
Immunoprecipitation
Molecular Biology
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Medusa Protocol
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Quick Transformation
quicke extraction from bacteria
RNA extraction Tryzol
Top10 competent cells
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Annexin V - PI Staining
Haemolysis assay
IF (suspension cells)
Intracellular staining for FACS
J chain folding assay
JetPEI transfection
Lentiviral Packaging
MPA Medium
Mycoplasma detection by PCR
Mycoplasma test from Blasi lab
NSO J558L electroporation
PEI
Reoxidation and trypsin digestion IP3R
Subcloning
Xbp1 splicing (EvA lab)
Myc detection PCR
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Immunology
aCD3-stimulation
Atg5 PCR
B cell purification
CFSE
Intracellular Staining
NP immunization and serum ELISA
NP-ELISA
tail DNA extraction
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Crostata di marmellata
Linzer Torte Originale
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ERp44-/- CRISPR-KO HeLa cells
EvA Cells
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Ordini - Chiamate 5656 - Ott2016
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