MOPS

NuPAGE^® MOPS SDS Running Buffer

The NuPAGE^® MOPS SDS Running Buffer (20X) is available from Invitrogen

50 mM MOPS

50 mM Tris base

0.1% SDS

1 mM EDTA

pH 7.7

1. To prepare 500 ml of 20 X NuPAGE^® MOPS SDS Running Buffer, dissolve the following reagents to 400 ml ultrapure water:

MOPS 104.6 g

Tris Base 60.6 g

SDS 10 g

EDTA 3.0 g

1. Mix well and adjust the volume to 500 ml with ultrapure water.

2. Store at +4° C. The buffer is stable for 6 months when stored at +4° C.

3. For electrophoresis, dilute this buffer to 1X with water. The pH of the 1X solution is 7.7. Do not use acid or base to adjust the pH.