Stripping solution (NaOH)

after ECL - nitrocellulose

• • 10 min PBS-Tween

  • 10 min water

  • 20 min NaOH 0,2 N

  • 10 min water

  • 10 PBS-Tween

    • after ECL - PDVF or notrocellulose (from Abcam)

    • Stripping Buffer

    • 15g glycine

    • 1g SDS

    • 10ml Tween 20

    • adjust pH 2.2

    • up to 1L with water

      • 10 min stripping-buffer RT

      • 10 min new stripping-buffer RT

      • 10min PBS

      • 10 PBS-Tween