SDS PAGE GRADIENT
Pump speed 3.3 ml/min
Gel caster without the lower central red rubber
For 4 gels (thickness: 1.5 mm)
For 5 gels 0.75 mm or 3 gels 1.5 mm
Add 70% glycerol in the left part of the gradient maker and wait for the glycerl to raise to the glasses: then stop the pump.
Add 400 ml of Propan-2-olo to smooth the upper part of the gel.
Allow the gels to polymerize for 1 hour.