Recombinant DNA Technology

DSC – 5C

APMB 232P Practicals-VIII: RECOMBINANT DNA TECHNOLOGY (1 credit)

1. Agarose gel electrophoresis.

2. Isolation of total bacterial DNA.

3. Isolation of yeast DNA.

4. Isolation of DNA from red blood cells.

5. Isolation of plasmid DNA.

6. Restriction digestion.

7. Ligation.

8. Production of competent E. coli cells.

9. Transformation of bacteria and isolation of transformants.