SEMESTER – II
DSC – 3B
APMB 123 CLINICAL BIOCHEMISTRY (3 credits)
(Biochemistry - II)
Unit - I
Basic concepts of clinical biochemistry: A brief review of units and abbreviations used in expressing concentrations and standard solutions. Biological samples – specimen collection – anticoagulant – preservatives for blood and urine – transport of specimens.
Unit - II
Disorders related to carbohydrate metabolism: diabetes mellitus – definition – WHO criteria – classification of diabetes mellitus – signs, symptoms and complications – GTT– galactosemia, galactosuria, fructosuria.
Unit - III
Disorders related to amino acid and lipid metabolism: Inborn errors of metabolism – phenylketonuria, alkaptonuria, albinism and tyrosinosis. Exogenous and endogenous transport of lipids – chylomicron transport, VLDL transport – reverse cholesterol transport – atherosclerosis – fatty liver – risk and anti risk factors.
Unit - IV
Liver function test: detoxification and excretory functions – protein changes in liver disease- differential diagnosis of jaundice – hemolytic hepatic and obstructive jaundice – Bilirubin metabolism, bile pigment levels in blood and urine – gastric function test – fractional test meal analysis and its interpretation. GI hormones – gastrin, secretin, CCK and gastric inhibitory peptide.
Unit - V
Diagnostic enzymology: plasma enzymes – functional and non-functional enzymes – isoenzymes – enzyme patterns in acute pancreatitis, liver diseases and myocardial infarction.
Text Books
1. Ahmed N. (2010) Clinical Biochemistry. Oxford University Press.
2. Vasudevan D.M. (2008) Textbook of Biochemistry for Medical Students, 5th Edn, Jaypee publishers.
Reference Books
1. Rodwell V.W., Bender D.A., Botham K.M., Kennelly P.J. and Weil P.A. (2015) Harper’s Biochemistry, 30th Edn. McGraw Hill Education.
2. Sathya Narayana U. (1999) Biochemistry, 2nd Edn. Kolkata, Allied Publishers.
3. Mallikarjuna Rao N. (2002) Medical Biochemistry, 2nd Edn. New Age Publishers.