SEMESTER – I
DSC - 1A
APMB 111 FUNDAMENTALS OF MICROBIOLOGY (3 credits)
Unit - I
History and scope of microbiology, spontaneous generation – biogenesis theory – contributions of Leeuwenhoek, Louis Pasteur, Robert Koch, Edward Jenner, Paul Ehrlich and Fleming.
Unit - II
Microscope – principles and application – simple and compound microscope – dark field – phase contrast, fluorescent microscope, SEM and TEM. Types of staining – simple, differential (Gram’s, AFB), special – capsular staining (negative), spore, LPCB, KOH mount.
Unit - III
Ultrastructure of bacteria, cell envelope, cell wall – Gram positive and Gram negative bacterial cell wall, slime, flagella, capsule, pili.
Unit - IV
Sterilization and disinfection – principles – methods of sterilization – physical methods – dry heat – moist heat – radiation – filtration (membrane and HEPA) – chemical sterilization – chemical agents – mode of action. Preservation and maintenance of culture
Unit - V
Culture and media preparation – solid and liquid. Types of media – semi synthetic, synthetic, enriched, enrichment, selective and differential media. Pure culture techniques – tube dilution, pour, spread, streak plate. Anaerobic cultivation of bacteria.
Text Books
1. Willey J.M., Sherwood L.M. and Woolverton C.J. (2013) Prescott’s Microbiology, 9th Edn. McGraw-Hill Higher Education.
2. Madigan M.T., Bender K.S., Buckley D.H., Sattley W.M. and Stahl D.A. (2017) Brock Biology of Microorganisms, 15th Edn. (Global Edn.) Pearson Education.
Reference Books
1. Talaro K.P. and Chess B. (2012) Foundations in Microbiology, 8th Edn. The McGraw Hill Companies.
2. Tortora, G.J., Funke, B.R. and Chase C.L. (2013) Microbiology: An Introduction, 11th Edn. Pearson-Benjamin Cummings.
3. Brown A. and Smith H. (2015) Benson’s Microbiological Applications: Laboratory Manual in General Microbiology, 13th Edn. McGraw-Hill Companies.