DSC – 4A
APMB 111P Practicals – I: FUNDAMENTALS OF MICROBIOLOGY (1 credit)
1. Handling of instruments and laboratory safety measures.
2. Handling and maintenance of compound microscope.
3. Cleaning of glassware and preparation of cleaning solutions.
4. Staining techniques a. simple, b. differential staining (Gram’s and Ziehl-Neelsen), c. special staining (spore and capsular staining methods) and wet-mount.
5. Hanging drop technique – motility.
6. Handling of laboratory instruments a. autoclave b. hot air oven c. laminar air flow d. pH meter.
7. Media preparation: a. liquid media – peptone water, nutrient broth; b. solid media – nutrient agar (agar slant, agar plate); c. enriched medium – blood agar; d. differential medium – Mac Conkey agar; e. enrichment medium – selenite F broth; f. selective medium – EMB, MSA.