SEMESTER – VI
DSE – 4B
APMB 365 HAEMATOLOGY AND BLOOD BANKING (4 credits)
Unit - I
Blood: definition, characters, composition. Collection of blood – capillary blood: from adults and infants, examinations employed. Venous blood: from adults and infants, examinations employed – Anticoagulants: definition – type: Wintrobes, EDTA, heparin, citrate, concentration, examinations, advantages and disadvantages.
Unit - II
Counting of blood cells: Neubauer counting chamber – total RBC count: diluting fluids, normal values – total WBC count: diluting fluids, normal values differential leucocyte count: granulocyte and agranulocytes, morphology and function, staining technique – Platelet count: morphological characters and functions, haemoglobin: composition and normal values, haemoglobin estimation.
Unit - III
Coagulation mechanism: factors, bleeding time, clotting time. Haematological indices: packed cell volume. Erythrocyte sedimentation: principle – determination: introbes, Westegren method – advantages and disadvantages – factors affecting the process.
Unit - IV
Preparation of stains and staining techniques: Wright stain, Leishman’s stain, Giemsa's stain, Fields stain, peroxidase stain. Examination of blood smear – peripheral smear report – size, colour and shape. Blood parasites: malarial parasite and microfilaria.
Unit - V
ABO Grouping: History, slide and tube technique, Rh typing: slide and tube technique, Coombs test: direct and indirect method, donor screening – cross matching, collection of blood, preservation and storage.
Text Books
1. Maheswari N. (2008) Clinical Pathology, Haematology and Blood Banking (for DMLT students), 2nd Edn. Jaypee Brothers Medical Publishers.
2. Hoffbrand A.V. and Moss P.A.H. (2015) Hoffbrand’s Essential Haematology, 7th Edn. Wiley.
Reference books
1. Greer J.P., Foerster J., Lukens J.N., Rodgers G.M., Paraskevas F. and Glader B.E. (Ed.) (2013). Wintrobe’s Clinical Hematology, 13th Edn. Wolters Kluwer.
2. HIllyer C., Silberstein L., Ness P., Anderson K. and Roback J. (2006) Blood banking and Transfusion medicine, 2nd Edn. Elsevier Press.
3. Godkar P.B. and Godkar D.P. (2013) Textbook Medical Laboratory Technology Vol-I and II, Bhalani Publishing House.