SEMESTER – VI
GE -2
APMB 367 MICROBIAL GENOMICS (3 credits)
Unit-I
Introduction to microbial genomics - sequencing genomes – first generation DNA sequencing – shot gun sequencing – second generation DNA sequencing – third and fourth generation DNA sequencing
Unit-II
Biological sequences as information – DNA, RNA and protein as informative molecules – general characteristics of microbial genomes – genome assembly – genome annotation – identification of an open reading frame in a genome.
Unit-III
Microbial genomes size and content – small genomes and large genomes – genomes of organelles – symbionts and organelles – eukaryotic microbial genomes an introduction: genomes of microbial parasites – the yeast genome.
Unit-IV
Functional genomics: microarrays and transcriptomes – gene chips and gene expression and its applications – RNA sequence analysis – methods in proteomics – comparative genomics and proteomics – the interactome.
Unit-V
Culture independent studies of microorganisms – metagenomics: principles and applications – steps in construction of a metagenomes – examples of metagenomic studies – metagenomics as a tool to reveal the vast microbial diversity.
Text Books
1. Madigan M.T., Bender K.S., Buckley D.H., Sattley W.M. and Stahl D.A. (2017) Brock Biology of Microorganisms, 15th Edn. (Global Edn.) Pearson Education.
2. Sanders E.R. and Miller J.H. (2010) I Microbiologist: A discovery based course in Microbial Ecology and Molecular Evolution, ASM press.
Reference Books
1. Fraser C.M., Read T. and Nelson K.E. (2004) Microbial Genomes, Springer.
2. Highlander S.K., Rodriguez-Valera F. and White B.A. (2015) Encyclopedia of Metagenomics: Environmental Metagenomics, Springer Science.
3. Dale J.W. and von Schantz M. (2007) From Genes to Genomes: Concepts and Applications of DNA Technology, 2nd Edn. Wiley publishers.