DSC – 4B
APMB 121P Practicals– IV: MOLECULAR BIOLOGY (1 credit)
1. Introduction to molecular biology laboratory techniques and development skills.
2. Elaboration of DNA and RNA structure and forms using micrograph or animation.
3. Working on Agarose gel electrophoresis.
4. DNA isolation from E .coli.
5. DNA estimation – spectrophotometric method.
6. UV irradiation – Percent survival curve.
7. Transformation (demonstration).
8. Isolation of plasmids (demonstration).
9. Isolation of RNA from cells (demonstration).
10. Identification of bacterial phylogeny using 16S rRNA gene sequencing (demonstration).