SEMESTER – III
DSC – 1C
APMB 231 BACTERIAL PHYSIOLOGY AND METABOLISM (3 credits)
Unit - I
Nutritional classification – importance of various macro, micro elements and growth factors in bacterial growth – bacterial growth curve – measurement of microbial growth – gravimetry, turbidometry and nephlometery. Continuous culture – synchronous culture – sporulation.
Unit-II
Transport of nutrients – uptake of nutrient – passive diffusion – facilitated diffusion – active transport (periplasmic binding protein and ABC transport) simple transport (uniport, symport and antiport) – group translocation and protein export system. Role of osmoregulatory proteins – permiomics.
Unit - III
Biosynthesis of cell structures from glucose (cell wall, capsule, flagella structure and synthesis, cell inclusions) – biochemistry of nitrogen fixation – nitrogenase enzyme – nitrogen assimilation – sulfate assimilation – anaplerotic reactions in the catabolic pathways.
Unit - IV
Photosynthesis – characteristics and metabolism of autotrophs, anoxygenic photosynthetic bacteria and cyanobacteria – CO2 fixation and mechanism of photosynthesis – chemotlithotrophs – hydrogen bacteria – nitrifying bacteria, sulphur bacteria and iron bacteria – methanogens – methylotrophs.
Unit - V
Central catabolic pathways – glycolysis, hexose monophosphate pathway, Entner Doudoroff pathway, tricarboxylic acid cycle – electron transport system–components – adenosine tri phosphate structure and their generation types – fermentations – types – anaerobic respirations.
Text Books
1. Drummond J.T., White D. and Fuqua C. (2012) The Physiology and Biochemistry of Prokaryotes, 4th Edn. Oxford University Press.
2. Kim B.H. and Gadd G.M. (2008) Bacterial Physiology and Metabolism, Cambridge University Press.
Reference Books
1. Schaechter M. (Ed.) (2009) Physiology In: Encyclopedia of Microbiology, 3rd Edn. Academic Press.
2. Moat A.G., Foster J.W. and Spector M.P. (2002) Microbial Physiology, 4th Edn. Wiley-Liss Publishers.
WEBSITE - https://sites.google.com/site/microbialphysiologyoddsem/