DSC – 4C
APMB 231P Practicals – VII: BACTERIAL PHYSIOLOGY AND METABOLISM (1 credit)
1. Determination of growth curve of bacteria.
2. Bacterial population count by turbidity method.
3. Estimation of fungal growth by gravimetric estimation.
4. Effect of temperature on bacterial growth.
5. Effect of pH on bacterial growth.
6. Biochemical characterization of bacterial cultures: catalase, urease, cytochrome oxidase and sugar fermentation, citrate utilization, gelatin liquefaction, sulfide indole motility test.
7. Nitrification and denitrification by bacteria.
8. Biolog/API based biochemical tests (demonstration).