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Supranational TB Reference Laboratory in Santiago, Chile
Address:
La Seccion Micobacterias del Instituto de Salud Pública
Av. Marathon 1000 Ñuñoa, Santiago
Casilla 48 Correo Central
Código Postal 7780050
Santiago, Chile
Contacts:
Fabiola Arias Muñoz (farias@ispch.cl)
Angélica Scappaticcio Bordón (ascappaticcio@ispch.cl)
Alex Figueroa Muñoz (afigueroa@ispch.cl)
Website:
http://www.ispch.cl/biomedicos/subdepto_enf_infecciosas/microbacterias
Genotyping method Mycobacterium tuberculosis
Spoligotyping
MIRU-VNTR typing based on 15 and 24 locis
Structure and organization
Mycobacteria Laboratory depends on the Sub-Department of Infectious Diseases, Department Biomedical Laboratory, is part of the National Tuberculosis Laboratory Network what includes 193 laboratories, linked by common objectives of the National Program for Control and Elimination of Tuberculosis (PROCET). These laboratories are classified in different levels of complexity, 154 primary health units, 39 at an intermediate level. Each level has different tasks.
Services provided by the SRL
It is a technical reference laboratory in Susceptibility and identification of mycobacteria .
Meets regulatory functions, training , coordination and direct supervision.
Performs Quality Control to Intermediate Laboratories of the National Public Network and private laboratories that adhere voluntarily .
Consolidates information from tuberculosis laboratory network , which are broadcast annually in workshops and seminars organized by the National Program for Control and Elimination of Tuberculosis (PROCET)
In charge of Surveillance of primary and acquired drug resistance and participates in operational epidemiological research.
Current links and partnerships outside of SRLN
Susceptibility profile:
• Proportional method in Lowenstein Jensen to first and second line drug
• Proportional method in MGIT 960 to first, second and alternative drugs
• Wayne method
LPA GenoType MTBDRplus VER 2.0 and GenoType MTBDRsl VER 2.0 Sequencing of gene rpoB, kat G, inh A, pnc A.
Identification of mycobacterias at level species:
• Lateral immune-chromatography technique
• Phenotypical methods
• Sequencing of genes hsp65 gene
LPA Genotype CM, AS and MTB Complex
Description
Mycobacteria Laboratory has its origins in 1957, when the Department of Tuberculosis was established in the Bacteriological Institute of Chile. It was in this laboratory where operational and epidemiological studies that gave way to the establishment of the first rules of use of diagnostic tools for tuberculosis. The responsibilities of our Laboratory at a National level are as a Reference Laboratory for the TB laboratories network and as a Reference Laboratory for mycobacteriosis.
We are mainly focused on the study of tuberculosis, and it provides technical assistance to National Control Programs of Tuberculosis.
Our laboratory’s main activities are:
• Reference diagnosis
• Surveillance of resistance anti-tuberculosis drugs
• Establishing of policies and standards
• Training of human resources
• Validation of methods
• Quality management and biosafety
TB diagnostic methods used in the laboratory
Additional information:
Research activities
ISP regular budget
- Evaluation of LPA GenoType MTBDRplus VER 2.0 and GenoType MTBDRsl VER 2.0 in direct sample.
- Validation of LED fluorescence stain and implementation in the laboratory network
- Sequencing of the pnc A gene for Pirazinamida
- Characterization of MDR and RR Mycobacterium tuberculosis strain from Chile by MIRU-VNTR, spoligotyping and whole-genome sequencing.
-Evaluation of transport reagent OMNIgene SPUTUM in the laboratory network.
Publications
Resistencia primaria a fármacos anti-tuberculosos en Chile 2011-2012. 2015. Fabiola Arias, Angélica Scappaticcio, Tania Herrera. Rev Chilena Infectol 2015; 32 (4): 382-386
Frecuencia de micobacterias ambientales en Chile en el año 2008. 2011. Angélica Scappaticcio B.*, Maritza Velasco R.*, Tamara Leiva C.* y Juan Carlos Rodríguez D**. * Sección Micobacterias, Laboratorio Biomédico Nacional, Instituto de Salud Pública de Chile. ** Instituto Nacional del Tórax. Rev. chil. enferm. respir. vol.27 no.3
Evaluación de una técnica de hibridación reversa para identificación rápida de micobacterias en Chile. 2012. Tamara Leiva C. Rev. chil. enferm. respir. vol.28. no. 1.
Rifampicin-resistant Mycobacterium tuberculosis: susceptibility to isoniazid and other anti-tuberculosis drugs. 2012. Kurbatova, E. V.; Cavanaugh, J. S.; Shah, N. S.; Wright, A.; Kim, H.; Metchock, B.; Van Deun, A.; Barrera, L.; Boulahbal, F.; Richter, E.; Martín-Casabona, N.; Arias, F.; Zemanova, I.; Drobniewski, F.; Santos Silva, A.; Coulter, C.; Lumb, R.; Cegielski, J. P. NT J TUBERC LUNG DIS 16(3):355–357.
Detección de mutaciones asociadas a cepas multidrogoresistentes de Mycobacterium tuberculosis en Chile. 2011. Pamela Araya, Maritza Velasco, Javier Tognarelli, Fabiola Arias, Tamara Leiva, Angélica Sccapatticio, Pablo Alviz, Jorge Fernández. Rev Med Chile; 139: 467-473.
Identificación de Micobacterias no Tuberculosas en Chile. 2006. Araya P., Tognarelli J., Leiva T., Arias F., Velasco M., Fernández J.
Trainings
We are making the training in internships in different topics according to demand of the laboratories
Annually we are organizing four or five courses in different activities according to demand the tuberculosis laboratories network
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