Gauting, Germany SRL

Description

The SRL Gauting is member of the SRL network since its foundation in 1994. It is partner of the National Reference Laboratories of Tajikistan, Uzbekistan, Turkmenistan, Novosibirsk Tuberculosis Research Institute (Russian Federation) and Kyrgyzstan, as well as Nepal and of the regional reference laboratories of Santa Cruz de la Sierra, Bolivia. 

Structure and organization

The SRL Gauting has three departments: 

1. The Kuratorium Tuberkulose in der Welt e.V. is an NGO supporting STOP TB projects in Kathmandu, Nepal, and Santa Cruz de la Sierra, Bolivia, since more than 25 years through donations only. All members are working in an honorary capacity; 

2. The IML red GmbH (IML = Institute of Microbiology and Laboratory medicine; red = research, education, development) represents the department of professional development assistance and research in tuberculosis; 

3. The IML Diagnostic MVZ offers medical laboratory diagnostics including TB diagnostics, general microbiology, virology, molecular biology, clinical chemistry, haematology, cytology, and immunology.

The laboratory rooms of the IML Gauting were newly refurbished from 2007 to 2009. It disposes of a modern, 93 m² High-Risk TB (TB Containment) laboratory, additional 100 m² BSL-2 laboratory rooms and six bio-safety-cabinets for TB diagnostics and research. The IML Gauting is ISO EN DIN 15189-2008 accredited by the German DAkkS accreditation authority. 

Total staff providing technical assistance for partner laboratories and on-site trainings - 8 persons (1 MD, 3 PhD, 1 clinical microbiologist, 1 biologist, 2 lab technicians). Number of experts with special competencies:

• 2 - Planning of new medium and high risk TB laboratories including BSL-3 units and ventilation systems;

• 2 - Control & certification of biosafety cabinets and ventilation systems;

• 3 - Project management;

• 3 - Quality Management System development;

• 7 - Technical On-site trainings

Our staff have the ability to speak:

• Native/Fluent: German, English, Russian, Belarusian, Bulgarian, French

• Basic level: Spanish, Italian

Services provided by the SRL

1. Guidance in Development of National TB Laboratory Strategic Plans and GF concept notes;

2. Development and implementation of TB laboratory diagnostic algorithms;

3. Elaboration and implementation of ISO 15189 QMS;

4. Planning of new medium and high risk TB laboratories including BSL-3 units and ventilation systems;

5. Practical on-site trainings in WHO-endorced TB laboratory diagnostics;

6. Annual external quality assessment of microscopy and DST (panel testing).

Procedures performed at SRL:

    Microscopy (ISO 15189:2013 accredited)

• Ziehl-Neelsen  microscopy

• Fluorescent microscopy (Auramin)

    Culture (ISO 15189:2013 accredited)

• Solid media (LJ)

• Liquid media by  automated method (BACTEC MGIT 960)

    Molecular tools (all assays ISO 15189:2013 accredited)

• Line-Probe Assays (GenoType® MTBDRplus, GenoType® MTBDRsl, GenoType® Mycobacterium CM, GenoType® MTBC )

• BD Probe Tec

• Roche Taqman

• Xpert MTB/RIF

• Spoligotyping, IS6110 RFLP

• Several sequencing assays for species identification, resistance markers etc.

    DST (ISO 15189:2013 accredited)

• 1st line:  Rapid liquid culture (MGIT), 

• 2nd line:  

  • Rapid liquid culture (MGIT), 

  • Solid culture (LJ proportion method),  

• WHO group V drugs: Delamanid, Bedaquiline, Linezolid, Clofazimine and others: available with different assays including MGIT 

• For all anti-TB and NTM drugs: Determination of minimal  inhibitory concentraions in resazurine microtiter assay (REMA)/TREK

• DST of non-tuberculous mycobacteria: 

  • REMA

  • TREK RGM

  • TREK SGM

  • Proportional method

  • MGIT

    Sequencing of targeted resistance genes (Sanger)

    Other

• QuantiFERON-TB Gold IT

• QuantiFERON-TB Gold plus

Current links and partnerships outside of SRLN