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National Center of Excellence - Supranational TB Reference Laboratory (NCE-SRL) in Novosibirsk, Russian Federation
Address:
FSBI Novosibirsk Tuberculosis Research Institute, MoH
630040, Okhotskaya street, 81A
Novosibirsk, Russian Federation
Contacts:
Andrey G. Cherednichenko (bact.nniit@gmail.com)
Irina Felker (felkeririna.nniit@gmail.com)
Website:
Description
Microbiological laboratory is a part of the Federal State Budgetary Institution "Novosibirsk Tuberculosis Research Institute" Ministry of Health of Russian Federation (NTRI). The laboratory is located in a separate building, total laboratory area is 614.4 m2. Laboratory provides supervision, methodological assistance and trainings for laboratory staff from 104 TB laboratories in Siberian and Far Eastern Federal districts, 22 of those are performing DST for MTB. It is also providing methodological support to 896 laboratories of general medical network (only for fluorescence microscopy and Ziehl-Nielsen) in Siberian and Far Eastern Federal districts.
Structure and organization
The laboratory includes two divisions: bacteriological laboratory and molecular-genetic laboratory. Bacteriological laboratory performs microscopic and cultural studies, bacteriological tests to monitor the microbiological state of the environment (sanitary microbiology) and also culture for nonspecific microflora.
Services provided by the SRL
1. A full range of microbiological and molecular genetic methods for TB diagnosis and follow-up during the chemotherapy in NTRI patients. Identification of MTB isolates by molecular genetic methods, the method of mass spectrometry. DST for the first and second line drugs.
2. Performing of reference microscopic, bacteriological, molecular genetic studies in a framework of tuberculosis.
3. The organizational and methodological assistance in the organization of TB laboratory services in supervised regions.
4. Conducting of trainings on TB laboratory diagnosis and biosafety for laboratory staff of TB services from Siberian and Far Eastern Federal districts.
5. External quality control in a framework of TB laboratory diagnosis.
TB diagnostics available
1. Microscopic methods: light microscopy with the Ziehl-Neelsen color / fluorescence microscopy with fluorochrome color.
2. The culture diagnostics: solid egg media / liquid culture medium in the system Bactec MGIT.
3. Drug sensitivity test: a method of absolute concentrations for the first and second line drugs / proportion method in a liquid medium in Bactec MGIT system for the first-line drugs, pyrazinamide, second-line drugs / modified method of proportions with tablet cultivation (Sensititre MYCOTB, RAPMYCO, SLOMYCO )
4. Molecular-genetic methods: Cartridge technique GeneXpert MTB / RIF; hybridization on strips (HAIN species identification, identification of non-tuberculous mycobacteria, DST for the first and second line drugs); Real-time PCR (DNA detection of Mycobacterium tuberculosis, the identification of resistance to isoniazid, rifampicin, ofloxacin).
5. Mass spectrometry for non-tuberculous mycobacteria species identification.
6. Whole genome sequencing.
Current links and partnerships outside of SRLN
Additional information:
Research activities
Publications
1. Highest prevalence of the Mycobacterium tuberculosis Beijing genotype isolates in patients newly diagnosed with tuberculosis in the Novosibirsk oblast, Russian Federation. Dymova M.A., Kinsht V.N., Cherednichenko A.G., Khrapov E.A., Svistelnik A.V., Filipenko M.L. J Med Microbiol. 2011 Jul; 60 (Pt 7):1003-9. Epub 2011 Mar 24.
2. Molecular characteristics of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis isolates from the Russian Federation. Afanas'ev MV, Ikryannikova LN, Il'ina EN, Sidorenko SV, Kuz'min AV, Larionova EE, Smirnova TG, Chernousova LN, Kamaev EY, Skorniakov SN, Kinsht VN, Cherednichenko AG, Govorun VM.
3. Use of a mini-sequencing test, followed by the MALDI-TOF mass-spectrometric analysis to evaluate rifampicin and izoniazid resistance in Mycobacterium tuberculosis circulating in the Russian Federation. Afanas'ev MV, Ikriannikova LN, Il'ina EN, Smirnova TG, Larionova EE, Kuz'min AV, Chernousova LN, Kamaev EIu, Skorniakov SN, Kinsht VN, Cherednichenko AG, Govorun VM. Probl Tuberk Bolezn Legk. 2007;(7):37-42. Russian.
4. «A hybrid system for bioaerosol filtration and subsequent filter decontamination by ozone from various microbes including M. tuberculosis» V.A. Krasnov, A.G. Cherednichenko, T.I. Petrenko, R.A. Mitrofanov A.S. Safatov, O.V. Pyankov, S.A. Kiselev, S.G. Safonova, D.A. Trubitsyn, D.I. Kolokolov, A.I. Latkin
5. Complete Genome Sequence of a Novel Clinical Isolate, Mycobacterium abscessus Strain NOV0213. Dymova M.A. *, Alkhovik O.I., Evdokimova L.S., Cherednichenko A.G., Petrenko T.I. Ministry of Public Health and Social Development of The Russian Federation (NRIT), Novosibirsk Research Institute of Tuberculosis, Novosibirsk, Russia. In-press. Journal "Genome announcement". SAMN04103082: NOV0213 (TaxId: 36809)
6. Dymova M.A., Alkhovik O.I., Evdokimova L.S., Cherednichenko A.G., Petrenko T.I., Complete Genome Sequence of a Novel Clinical Isolate, Mycobacterium abscessus Strain, Genome Announc. 2016 Jan 7;4(1)
7. Cherednichenko AG, Dymova MA, Solodilova OA, Petrenko TI, Prozorov AI, Filipenko ML. Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis // PubMed, 2016.
URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=10.1007%2Fs10517-016-3243-3+
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