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Supranational TB Reference Laboratory in Mexico city, Mexico
Address:
al Laboratorio de Micobacterias del Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE)
Instituto de Diagnostico y Referencia Epidemiologicos (INDRE) “Dr. Manuel Martínez Báez”,
Francisco de P. Miranda 177 Col. Lomas de Plateros
Del. Álvaro Obregón
México. D.F. 01480
Contacts:
Claudia E. Backer (cebacker60@gmail.com)
Carolina Flores Marroquin (carolina.flores.marroquin@gmail.com)
José Alberto Diaz Quiñónez (alberto.diaz@salud.gob.mx)
Website:
Description
The Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE) “Dr. Manuel Martínez Báez” through the Mycobacteria Laboratory is the National Reference Laboratory (NRL) at the federal level in Mexico. It´s the head of the Red Nacional de Laboratorios de Salud Pública de TB [Public Health Laboratory Network –PHLN on TB -] for diagnosis, reference, training, updating and assessment of technical competence and it performs and coordinate technical, operational and epidemiological research related to tuberculosis. Also it works close to the National Tuberculosis Program.
Finally, the Mycobacteria Laboratory participates in the international Quality Control Program set by WHO.
Structure and organization
The technical, process and method used in the PHLN on TB are the same, following the “Guidelines for Surveillance Laboratory of Tuberculosis". This is the regulatory framework established by the NRL.
The PHLN on TB has a pyramidal organization. It has three levels: on the top the NRL, in the middle (32 Public health State Laboratories) and on the bottom (local laboratories). Each of them have different administrative levels and diagnosis capacity but they are coordinated together to ensure access to special technical diagnosis.
Also, there are other laboratories from different health institutions offering the diagnosis test to support the National Tuberculosis Program.
Services provided by the SRL
Among the services offered by the NRL are the following:
• DST1L for laboratories that do not make it routinely and DST2L for all the country.
• Training for the PHLN staff on bacteriology techniques when needed, and updating courses.
• Performing quality control PHLN on TB through direct and indirect supervision and sending panels when needed.
• Collecting, analyzing and evaluating information on the activities of the PHLN on TB.
TB diagnostic methods available in the laboratory are the following:
• Microscopy staining by the Ziehl-Neelsen and fluorescence microscopy (Auramine O).
• Culture by modified Petroff method in solid Lowenstein-Jensen medium and liquid medium (MGIT 960) and Ogawa.
• Identification of the species M. tuberculosis by biochemical methods (niacin production / niacin strip, catalase and nitrate reduction at room temperature and at 68 ° C) or by ICL strip AgMPT64.
• DST 1L
BACTEC MGIT 960 TB.
Proportion method in Lowenstein-Jensen.
• DST2L by proportion method in Middlebrook 7H10. In process of implementation by BACTEC MGIT 960 TB.
• PCR in real time to identify M. tuberculosis and resistance to rifampicin (Xpert MTB RIF Test). In process of implementation.
• Wayne test.
Current links and partnerships outside of SRLN
Additional information:
Research activities
These activities are offered in special situations (not for a regular diagnosis)
Mycobacterium tuberculosis strains genotyping by spoligotyping and 24 loci MIRU-VNTR.
Allele-specific PCR for Rapid Detection of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis strains
Publications
Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolates from high prevalence tuberculosis states in Mexico. Juarez-Eusebio DM, Munro-Rojas D, Muñiz-Salazar R, Laniado-Laborín R, Martinez-Guarneros JA, Flores-López CA, Zenteno-Cuevas R. Infect Genet Evol. 2016 Sep 13. pii: S1567-1348(16)30395-1. doi: 10.1016/j.meegid.2016.09.012. [Epub ahead of print] PubMed PMID: 27637930
Molecular epidemiology of Mycobacterium tuberculosis in Baja California, Mexico: A result of human migration. Flores-López CA, Zenteno-Cuevas R, Laniado-Laborín R, Reynaud Y, García-Ortiz RA, González-Y-Merchand JA, Rivera S, Vázquez-Chacón CA, Vaughan G, Martínez-Guarneros JA, Victoria-Cota NL, Cruz-Rivera M, Rastogi N, Muñiz-Salazar R. Infect Genet Evol. 2016 Jul 11. pii: S1567-1348(16)30279-9. doi: 10.1016/j.meegid.2016.07.001. [Epub ahead of print] PubMed PMID: 27418234.
Human multidrug-resistant Mycobacterium bovis infection in Mexico. Vazquez-Chacon CA, Martínez-Guarneros A, Couvin D, González-Y-Merchand JA, Rivera-Gutierrez S, Escobar-Gutierrez A, De-la-Cruz López JJ, Gomez-Bustamante A, Gonzalez-Macal GA, Gonçalves Rossi LM, Muñiz-Salazar R, Rastogi N, Vaughan G. Tuberculosis (Edinb). 2015 Dec;95(6):802-9. doi: 10.1016/j.tube.2015.07.010. Epub 2015 Aug 13. PubMed PMID: 26299906.
Estandarización de la Técnica de PCR Tiempo Real para la Diferenciación de las Especies de M. tuberculosis y M. bovis del CMTB. A. Rodríguez-Castillo, A. Mandujano Martínez, C.E. Bäcker, M. González-Villa, I.I. Martínez-Rivera, E. Andrade-Montiel, B.P. Torres Mazadiego, M. Torres-Cosme, L. Hernández-Rivas, J.E. Ramírez-González. Poster presentado en el 1er. Congreso Internacional de Biomedicina Molecular. Del 25-28 de Nov. 2015. Mazatlán, Sin. México
Circulation of multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) Mycobacterium tuberculosis strains in Mexico. A. Martinez-Guarneros, C.A. Vazquez-Chacon, S. Balandrano-Campos, J. Luna-Herrera, C.E. Bäcker, D. Lozano, L.M. Gonçalves-Rossi, K. Ruiz-Tovar, S. Fonseca-Coronado, G. Vaughan, A. Escobar-Gutierrez. American Journal of Infectious Diseases.10 (4). 174-178. DOI: 10.3844/ajidsp.2014.174.178.
Hsp65 Phylogenetic Assay for Molecular Diagnosis of Nontuberculosis Mycobacteria Isolated in México. N. Escobar-Escamilla, J.E. Ramírez-González, M. González-Villa, P. Torres-Mazadiego, A. Mandujano-Martínez, C. Barrón-Rivera, C.E. Bäcker, D.E. Fragoso-Fonseca, H. Olivera-Díaz, P. Alcántara-Pérez, A. Hernández-Solís, R. Cicero-Sabido, I.A. Cortés-Ortíz. Archives of Medical Research 45 (2014) 90-97.
Drug resistence in México: results from the National Survey on Drug-Resistant Tuberculosis. I.Bojorquez-Chapela, C.E. Backer, I. Orijel, A. López, A. Díaz-Quiñonez, M.I. Hernández –Serrato, S. Balandrano, M. Romero, M.M. Téllez-Rojo Solís, M. Castellanos, C. Alpuche, M. Hernández- Ávila, H. López-Gatell. Int. J. Tuberc. Lung Dis.17 (4):514-519. 2013. UICTER.
Multidrug-resistant tuberculosis among patients in Baja California, Mexico, and Hispanic patients in California. Bojorquez I, Barnes RF, Flood J, López-Gatell H, Garfein RS, Bäcker CE, Alpuche C, Vinetz JM, Catanzaro A, Kato-Maeda M, Rodwell TC. Am J Public Health. 2013 Jul;103(7):1301-5. doi: 10.2105/AJPH.2012.301039. Epub 2013 May 16. PubMed PMID: 23678924; PubMed Central
PMCID: PMC3682598.
Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico. Martinez-Guarneros A, Rastogi N, Couvin D, Escobar-Gutierrez A, Rossi LM, Vazquez-Chacon CA, Rivera-Gutierrez S, Lozano D, Vergara-Castañeda A, Gonzalez-Y-Merchand JA, Vaughan G. Infect Genet Evol. 2013 Mar;14:434-43. doi: 10.1016/j.meegid.2012.12.024. Epub 2013 Jan 16. PubMed PMID: 23333775.
Mycobacterium tuberculosis Complex Genotype Diversity and Drug Resistance Profiles in a Pediatric Population in Mexico. M. Macías-Parra, J. Kumate-Rodríguez, J.L. Arredondo-García, Y. López-Vidal, M. Castañón-Arreola, S. Balandrano, N. Rastogi, P. Gutiérrez-Castrellón. Tuberc Res Treat. 2011: 239042. Aug 23, 2011.
Multidrug-resistant pulmonary tuberculosis in Los Altos, Selva and Norte regions, Chiapas, México. H.J. Sánchez-Pérez, A. Díaz-Vázquez, J.C. Nájera-Ortiz, S. Balandrano, M. Martín-Mateo. Int. J. Tuberc. Lung Dis. 14(1):34-39. UICTER. 2010.
Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y Guía Técnica. Parte I. Baciloscopia. OPS/OMS. Lucia Barrera INEI, ANLIS Dr. Carlos G. Malbran, Argentina. Revisión
técnica: Susana Balandrano InDRE, México, María Cecilia Riquelme y Maritza Velazco INS, Chile, Ernesto Montoro IPK, Cuba, María Consuelo Garzón Torres INS, Colombia. 2008.
Manual para el diagnóstico bacteriológico de la tuberculosis. Normas y Guía Técnica. Parte II Cultivo. OPS/OMS. Lucia Barrera INEI, ANLIS Dr. Carlos G. Malbran, Argentina. Revisión técnica: María Delfina Sequeira INEI, ANLIS, Argentina, Susana Balandrano InDRE, México, Maritza Velazco INS, Chile, Ernesto Montoro IPK, Cuba. 2008.
Trainings
The training is on demand of the staff of the PHLN on TB
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