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National Center of Excellence Supranational TB Reference Laboratory (NCE-SRL) in New Dehli, India
Address:
National Reference Laboratory (RNTCP), Department of Microbiology
National Institute of TB and Respiratory Diseases
Sri Aurobindo Marg, Near Qutab Minar,
New Delhi - 110030, INDIA
Contacts:
Rohit Sarin, Director (r.sarin@nitrd.nic.in)
Vithal Prasad Myneedu, Microbiologist (SAG) (v.myneedu@nitrd.nic.in)
Website:
Description
The Microbiology Department of NITRD is an advanced centre for TB and all respiratory diseases with all facilities from microscopy to molecular tests. The Department has a distinction of being one of the National Reference Laboratories (NRL) under RNTCP of Government of India. As NRL, the Department provides diagnostic, training and operational research to the RNTCP. It carries out accreditation / certification services of various IRLs and C&DST laboratories in the allotted States of India. The Department is also involved in training of the postgraduates, lab technicians, Medical M.Sc and Medical B.Sc students for dissertation and internship.
Structure and organization
The Director is the Chief Executive Officer of the Institute. Department is having following staff :
Dr V.P. Myneedu (MBBS, MD), Microbiologist (SAG) & Head
Dr Manpreet Bhalla ( MBBS, MD) Senior Research Officer ( SAG Grade)
Dr Ajoy Verma( MBBS, MD) Specialist Microbiologist
Dr Ritu Singhal ( MBBS, MD) Specialist microbiologist
NRL Staff
Dr Niti Singh ( MSc, PhD) NRL Microbiologist
Dr Jyoti Arora ( MSc, PhD) NRL Microbiologist
Dr. Himanshu Jha, NRL Microbiologist
Dr Gavish Kumar ( MSc, PhD) FIND Microbiologist
Services provided by the SRL
Department of Microbiology have following sections :
1. Mycobacteriology :
a) Provides diagnostic services (smear, culture, DST and molecular as well as epidemiological) to out patient department and 470 bed indoor facility.
b) Provides monitoring and follow up services to RNTCP and PMDT as required.
2. Bacteriology :
a) Provides diagnostic services (smear, culture, DST and molecular as well as epidemiological) to out patient department and indoor facility through its automated ID and DST system.
3. Mycology :
a) Provides Culture and Identification and Serological Diagnostic tests for various fungal diseases of respiratory tract.
4. Serology:
a) Carries out various serological tests for viral, fungal and bacteriological diseases especially for HIV, Hepatitis etc.
5. Research and Training :
a) Participates in various multi-centric evaluation of newer diagnostic modalities for evaluation.
b) Also carries out original research for development of newer diagnostic modalities as well as devices.
6. National Reference Laboratory :
a) Carries out monitoring activities for RNTCP/ PMDT in the States of Haryana, Delhi, Punjab, Himachal Pradesh, Jammu & Kashmir, Chandigarh and Bihar.
b) Provides diagnostic and follow up services for MDR/XDR-TB.
c) Provides and carries out proficiency testing of IRLs and C&DST Labs.
d) Carries out Training of Trainers in various laboratory modalities including infection control.
TB diagnostic methods available in the laboratory
Smear Microscopy
Culture (Solid and Liquid)
DST (Solid and Liquid)
Line Probe Assay
Epidemiological Typing
Testing of Newer Compounds for anti-mycobacterial properties
Culture and Identification of all non-tubercular mycobacteria
Current links and partnerships outside of SRLN
Provides consultancy services to SAARC for establishment and upgradation of SAARC Supra-national Reference Laboratory at Kathmandu, Nepal.
Carried out training of TB workers (Medical Officers and Laboratory Personnel) of South East Asia Region under IUATLD.
List of international collaborators
1. International Union against Tuberculosis and Lung Disease (IUATLD)
2. Medecins Sans Frontieres (MSF)
3. Henry Ford Hospital, Detroit (US)
Additional information:
Research activities
Multiplex PCR for rapid identification of mycobacterium species.(2012)
Pilot study for isolation and identification of mycobacterium avium in HIV patients.(2012)
Innate immunity markers :Role of toll-like receptors association of TLR4 with tuberculosis :Indian scenario.(2012)
Novel sample processing for the simple and rapid diagnosis TB, MDR-TB and XDR-TB.(2012)
Study of air quality in the wards , OPD and Labs of LRSI (A mycobacteriological study).(2012)
“Multicentric cohort study of recurrence of tuberculosis Among newly diagnosed sputum positive pulmonary Tuberculosis patients treated under RNTCP.”
“Validation of Indigenously Developed Technology for Diagnosis of Pulmonary. TB & Drug Resistant Tuberculosis”
“ The Association of Monocyte Chemotactic Protein 1(MCP1) and CC Chemokine Receptor 2(CCR 2)gene variants with tuberculosis.
Phenotypic and genotypic characterizatiuon of NON tuberculous Mycobacterium and its clinical relevance
Molecular characterization of rapid grower mycobacterium amongst the nontuberculous
Development and evaluation of line probe assay for rapid detection of flouroquinolone resistance in Mycobacterium tuberculosis
Validation of LPA for detecting resistant to fluoroquinolones ,Amimoglycosides & cyclic peptides in program seting in India using MTB DR SL test
Evaluation of MTBDR PLUS version 2.0 LPA for detection of MTB and drug resistance in EP Specimen
Pyrazinamide resistance in MTB isolate From MDR suspect and identification of mutations by sequencing
Standardization of cytogenetic profiling in Non-Small cell Lung cancer ( Non-Squamous ) Patients for therapeutic management
Genotypic characterization and phenotypic correlation of Rifampicin Resistant isolate with unknown mutations obtained in Genotype MTBDR plus assay
List of DNB Thesis Undertaken by the Dept of Microbiology
Clinical evaluation of tuberculosis viability microscopy for assessing treatment response
HIV seropositvity in the patients of bronchogenic carcinoma and COPD in NITRD
HIV seropositvity in Pulmonary TB suspects in NITRD
Prospective study of fungal colonization and invasive fungal disease in mechanically ventilated patients receiving long term antibiotics in the ICU, NITRD
To study the profile of chronic pulmonary aspergillosis among post-tubercular sequelae patients at NITRD
Publications
List of Publication since 2014
1. Microscopy as a diagnostic tool in pulmonary tuberculosis. Ritu Singhal, Vithal
Prasad Myneedu. International Journal of Mycrobacteriology 2015; 4(1):1-6.
2. Synergetic Effect of TLR4 Gene (D299G and T3991) Polymorphisms in
Susceptibility to Pulmonary Tuberculosis. Paras Singh, Mradula Singh, Manpreet
Bhalla, Rupak SInghla, Phani Kumar Gamma, Devika Tayal, Vithal Prasad Myneedu, Rohit Sarin. Universal Journal of Medical Science 3(1):19-27, 2015
3. The clinical utility of polymerase chain reaction and adenosine deaminase
(ADA), for the diagnosis of pleural tuberculosis : Indian scenario. Singh P, Singh M, Tayal D, Myneedu VP, Bhalla M, Adlakha P, Sarin R. Immunology and Infectious
Diseases. 2014;2(2):13-21
4. Development of POC test for TB based on multiple immunodominant epitopes
of MTB speci냗뫢c cell wall proteins. Gonzalez M, Francis B, Burda S, Hess K, Behera D, Gupta D, Agarwal AN, Verma I, Verma A, Myneedu VP, Biedbala S, Laal S. PLoS One.2014 S23;9(9):e106279
5. Identification of Mycobacterial species by PCR resection enzyme analysis of the hsp65 gene – An Indian Experience. Verma AK, Kumar G, Arora J, Singh P, Arora VK, Myneedu VP, Sarin R. Can J Microbiol. 2015; 61(4): 293-6.
6. Xpert W MTB/RIF assay for tuberculosis diagnosis : Evaluation in an Indian
setting. Myneedu VP, Behera D, Verma AK, Bhalla M, Singh N, Arora J, Singhal R,
Mathur M, Lal P, Sarin R. Int J Tuberc Lung Dis 2014; 18(8):958-60.
7. Phylogenetic associations with drug-resistant Mycobacterium tuberculosis
isolates in a paediatric population. Arora J, Sidiq Z, Sharma S, Singhal R, Bhalla M, Couvin D, Sarin R, Rastogi N, Myneedu VP. Int J Tuberc Lung Dis 2014; 18(10):1172-
8. Impact of introducing the line probe assay on time to treatment initiation of
MDR-TB in Delhi, India. Singla N, Satyanarayana S, Sachdeva KS, Van den Bergh R, Reid T, Tayler-Smith K, Myneedu VP, Ali E, Enarson DA, Behera D, Sarin R. PLoS One. 2014 24;9(7):e102989
9. Early detection of multi-drug resistance and common mutations in
Mycobacterium tuberculosis isolates from Delhi using GenoType MTBDRplus assay. Singhal R, Myneedu VP, Arora J, Singh N, Bhalla M, Verma AK, Sarin R. Indian J Med Microbiol 2015; 33(Supplement 1) : S416-52.
10. Multi-drug resistance tuberculosis among category-I treatment failures – A
retrospective study. Singh N, Sidiq Z, Bhalla M, Myneedu VP, Sarin R. Indian J
Tuberc. 2014; 61(2):148-51.
11. Detection of multi-drug resistance & characterization of mutations in
Mycobacerium tuberculosis isolates form North-Eastern States of India using
GenoType MTBDRplus assay. Singhal R, Myneedu VP, Arora J, Singh N, Sah GC,
Sarin R. Indian J Med Resp. 2014; 140:501-6.
12. Analysis of cross resistance between kanamycin and amikacin in extremely
drug resistance tuberculosis isolates. (Abstract in “IJTLD” on proceedings of the
45th Union World Conference on Lung Health 2014 held at Barcelona, Spain from
28th October to 01st November 2014). Verma AK, Kumar G, Arora J, Myneedu VP, Sarin R. International Journal Tuberculosis Lung Disease 2014;185(11):S281.
13. Ofloxacin resistance among Mycobacerium tuberculosis isolates from
retreatment cases at a tertiary care institute. (Abstract in “IJTLD” on proceedings of the 45th Union World Conference on Lung Health 2014 held at Barcelona, Spain from 28th October to 01st November 2014). Arora J, Verma AK, Kumar G, Sarin R, Myneedu VP. International Journal Tuberculosis Lung Disease 2014;185(11):S577.
14. Isolation and identi냗뫢cation of non-tuberculosis mycobacterium by polymerase chain reaction: Restriction enzyme – a simple and robust method. (Abstract in “IJTLD” on proceedings of the 45th Union World Conference on Lung Health 2014 held at Barcelona, Spain from 28th October to 01st November 2014). Verma AK, Kumar G, Arora J, Bhalla M, Panchal S, Myneedu VP, Sarin R. International Journal Tuberculosis Lung Disease 2014;185(11):S107.
15. Profile of TB among household contacts of MDR-T patients in Delhi. India.
(Abstract in “IJTLD” on proceedings of the 45th Union World Conference on Lung
Health 2014 held at Barcelona, Spain from 28th October to 01st November 2014). Singla R, Singla N, Myneedu VP, Behera D, Sarin R. International Journal
Tuberculosis Lung Disease 2014;185(11):S339-40.
16. First and second line drug resistance among treatment naïve pulmonary
tuberculosis patients in a district under Revised National Tuberculosis Control
Programme (RNTCP) in New Delhi. Vithal Prasad Myneedu, Ritu Singhal, Khalid
Umar Khayyam, Prem Prakash Sharma, Manpreet Bhalla, Digambar Behera, Rohit
Sarin. Journal of Epidemiology and Global Health : May 2015.
Further list will be provided on request and please refer website of the Institute for publications.
Trainings
Trainings as required under RNTCP
Smear Microscopy
EQA of Smear Microscopy
Culture and DST of M.tuberculosis by Solid Media LJ.
Culture and DST of M.tuberculosis by MGIT method (Liquid Culture)
LPA for DST frst line and II line drugs for M.tuberculosis
Mycobacteriology training for Med microbiology and Respiratory Medicine post
graduate students.
Laboratory trainings for Trainers under RNTCP.
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