A Goldador from the United States and the leader of the team. Hunter is optimistic, funny, devoted, friendly, level-headed, and an effective leader. Hunter's power is super speed; this allows him to run faster than the speed of sound. He lives with President Bill Clinton in the White House. He also has a flirtatious relationship with Colleen in the later episodes of the series, which the latter reciprocates.

A no-nonsense Rough Collie from the United Kingdom, and the only female member of the Road Rovers. She is the coordinator of the team, always checking to see if everything is going well without any problems or issues. Colleen is also very athletic, being a skilled martial artist. She currently resides with the prime minister of the United Kingdom, John Major and his wife Norma Johnson. She is openly attracted to Hunter, with whom she developed a flirtatious relationship during later episodes of the series. She was turned into a werewolf after getting attacked by werewolves she and the team were fighting in London and was later turned back to normal by the swamp water.

A Doberman from Germany. Unlike Hunter, Blitz is often selfish, immature, spiteful, faint-hearted, and has an ill temper. He has razor-sharp claws and strong jaws, which allow him to bite and cut through almost any substance. Blitz was formerly a guard dog for thieves, but now lives with the chancellor of Germany, Helmut Kohl. Blitz attempts to flirt with Colleen several times over the series, part of a running gag in which his flirting is met with her pretending not to know who Blitz is. This joke ran up until the series' final episode, at which point she finally acknowledge Blitz by name.

A Siberian Husky from Russia. His full name is Exilo Michalovitch Sanhusky. Exile speaks English with a thick Russian accent, sometimes mixing up words in the process (ex: "Aprilday" instead of "Mayday", "Jingle balls" instead of "Jingle bells"). He is friendly and easygoing, but often clashes with Blitz. Exile is super strong and also has heat, ice, and night vision. He used to work with other huskies as a sled dog, but now lives with the president of Russia Boris Yeltsin. Exile and Blitz shared a running gag in which Blitz would make a comment that could be interpreted in various ways, to which Exile would reprimand Blitz for being a 'weird boy.'

A cowardly Old English Sheepdog from Switzerland. Shag only transformed halfway into a Cano-sapien. He walks like a human, but speaks in a half-dog, half-human dialect. In addition to this, he does not wear a uniform, instead remaining in his natural state. Like Exile, Shag is super strong, and also his hair can store a lot of things like weapons and random items. He lived in a valley with a flock of sheep, but now lives with Arnold Koller, the president of the Swiss Confederation. Despite his strength and ability to store extreme amounts of weaponry, Shag is extremely fearful, and often hides behind the other Rovers when confronted by danger.

A shy but kind, sweet, lovable and curious Rottweiler. Muzzle was once "Scout", Professor Shepherd's dog that was kidnapped. He was never transformed into a Cano-sapien, but instead became aggressive due to Parvo's failed experiment on him. Due to this aggression, he is often restrained on a cart while wearing a straitjacket and mask.

An Afghan Hound and commander of the Space Rovers.

A Bloodhound scientist.

A wise dog in the mountains.

The geneticist behind the transdogmafier and the Road Rovers. He's the master of the Road Rovers, and the one who selected each of them. Without him, the world would have been ruled by the cano-mutants led by Parvo.

The Road Rovers' main antagonist who is out to destroy Professor Shepherd and conquer the world. He has a permanent cough. It was revealed later in the series that General Parvo was at one time a Cat before being transformed into a "Feline Sapien", which resemble humans a great deal more than Shepherd's Cano-sapiens. He shares a very similar appearance to wrestler Hulk Hogan. The name Parvo derives from a canine disease which often kills puppies.

The Groomer is the mostly loyal assistant to General Parvo. She's generally armed with a portable hair clipper, though she uses other equipment if it's appropriate.

Dogs turned into humanoid monsters and led by Parvo.

A pack of werewolves appearing in "A Hair of the Dog That Bit You" that turned Colleen into one and were restored by Hunter with the swamp water.