The thickness of the bottom glass is covered by the second set of calculations, but does not cover an aquarium which has a bottom glass that is well supported from below the aquarium in an even uniform manner. The surface must be very level. On very large aquariums this can be difficult to achieve and self-leveling filler may be needed between the polystyrene and the base. This should be applied just prior to fitting the aquarium to the base so that the aquarium’s weight levels out imperfections. Significant time must be allowed for the filler to fully cure before the aquarium is filled. If the bottom glass is only to be supported by all four edges then use the second set of calculations. The same thickness glass can be used on a uniformly supported bottom as well and this will significantly improve the safety factor. If the aquarium is to be supported from below in a uniform distributed manor, then the same thickness glass that is used for the largest side panel may be used. To do so requires the supporting base to support part of the load so therefore it must be VERY strong.
Source: https://aquariumworld.nz/fish/articles-guides/technical-diy/calculating-glass-thickness-for-aquariums-r23/