Lourival Domingos Possani

Lourival Domingos Possani, Brazilian-born Mexican toxicologist (Santo Angelo, Rio Grande do Sul State 09 July 1939 -

Authored 500 publications (with about 19,700 citations by Google Scholar, about 17,000 by ResearchGate and about16,600 by Research.com)

Described a number of novel toxins form scorpions, reptiles, molluscs, spiders and wasps

Described the toxic venom peptides that affect the function of ion channels of excitable cells

First to report peptides that block potassium (K+) channels (noxiustoxin 1986 and ergtoxin from Centruroides noxius)

Found peptide toxins with immune-modulatory function and that with antibiotic and antiparasitic activity

Other peptides with interesting biological activities such as specific toxins for insects and crustaceans

With F.Z. Zamudio discovered hadrurine (from Hadrurus gertschi) and scorpine (from Pandinus imperator) with microbicide activity (2000)

Isolated and characterized a peptide with antiepileptic activity from Centruroides limpidus limpidus (1994)

Designed and synthesized about a hundred peptides

OTHER CONTRIBUTIONS

With M.U. Naseem, E. Carcamo Noriega, J. Borrego, T.G. Szanto, F.Z. Zamudio, G. Delgado-Prudencio & G. Panyi. Cm28, a scorpion toxin having a unique primary structure, inhibits KV12 and KV13 with high affinity. J. Gen. Physiol. 154(8), 2022

With M. Benard Valle, E.E. Neri-Castro, M.F. Yañez-Mendoza, B. Lomonte, A. Olvera, F. Zamudio, R. Restano-Cassulini, E. Jimenez-Ferrer & A. Alagon. Functional, proteomic and transcriptomic characterization of the venom from Micrurus browni browni: identification of the first lethal multimeric neurotoxin in coral snake venom. J. Proteom. 225:103863, 2020

With F. Caliskan, V. Quintero-Hernandez, R. Restano-Cassulini, F.I. Coronas-Valderrama & G. Corzo. Molecular cloning and biochemical characterization of the first Na+- channel alpha-type toxin peptide (Acra4) from Androctonus crassicauda scorpion venom. Biochimie 95:1216-22, 2013

With L. Riano-Umbarila, V.R. Juarez-Gonzalez, T. Olamendi-Portugal, M. Ortiz-Leon & B. Becerril. A strategy for generation of specific human antibodies by directed evolution and phage dusplay. Febs J. 272:2591-01, 2005

With A. Shalabi, F. Zamudio, X. Wu, A, Scaloni & M.L. Villereal. Tetrapandins, a new class of scorpion toxins that specifically inhibit store-operated calcium entry in HEK-293 cells. J. Biol. Chem. 279:1040-9, 2004

With M. Corona, F.V. Coronas, E. Merino, B. Becerril, R. Gutierrez, S. Rebolledo-Antunez & D.E. Garcia. A novel class of peptide found in scorpion venom with depressant effects in peripheral and central nervous system of the rat. Biochim. Biophys. Acta Prot. Proteom. 1649:58-67, 2003

With F. Gómez Lagunas & T. Olamendi Portugal. Block of Shaker B K+ channels by Pi1, a novel class of scorpion toxin. FEBS Lett. 400:197-200, 1997

PAPERS IN TOP JOURNALS

With E. Carcamo-Noriega, S. Sathyamoorthi, S. Banerjee, E. Gnanamani, E. Mendoza Trujillo, D. Mata-Espinosa Montserrat, R. Hernandez Pardo, J.I. Veytia Bucheli & R.N. Zare. 1,4-benzoquinone antmicrobial agents against Staphylococcus aureus and Mycobacterium tuberculosis derived from scorpion venom. Proceed. Natl. Acad. Sci. 116(26):12642-7, 2019

Antivenom for scorpion sting. The Lancet 355:67, 2000

With M.M. Altamirano, C. Garcia & A.R. Fersht. Oxidative refolding chromatography: folding of the scorpion toxin Cn5. Nature Biotechnol. 17:187-91, 1999

With E. Carbone, E. Wanke, G. Prestipino & A. Maelicke. Selective blockage of voltage-dependent K+ channels by a novel scorpion toxin. Nature 296:90-1, 1982

With R. Banerjee, C. Balny & P. Douzou. Oxidation of haemoglobin by oxygen in light: possible role of singlet oxygen. Nature 226:861-2, 1970

PATENTS

With R.N. Zare, J.I. Veytia Bucheli, E.N. Carcamo Noriega, G. Elumalai, S. Sathyamoorthi, S. Banerjee & R.E. Hernandez Pando. Scorpion venom benzoquinone derivative and uses thereof US20210214303A1 EP3801765A1 CN112512635A (2021) WO2019231735A1 (2019)

With M.V. Prates, G.A. Joanitti, C. Bloch Jr., S.M. de Freitas, G.D. Brand, J.P.C. Fernandes, C.B.F. Mourão, J.A.R.G. Barbosa, E.N.F. Schwartz, M.R. Mortari, R.M.R. Cassulini & S.G. da Fonseca.Peptídeos inibidores de serinopeptidase modificados de peçonha de escorpião, seu processo de obtenção e uso BR102017013362A2 (2020)

With M.M. Pedraza Escalona, G.P. Espino Solis, A. Olvera Rodríguez & H.M. Cardoso Torres. Monoclonal antibodies against the DEC-205 receptor of chicken denditic cells US09951135B2 (2014) & MX346878B (2017)

With M.A. Abdel Rahman, A.A.M. Hassan, H. Hotta & M.M. Sarhan. Antiviral agent WO2017126340A1 (2017)

With V. Quintero, L. Gonzalez, J.M. Jimenez-Vargas, E. Ortiz, G. Corzo & B. Becerril.Composición farmaceutica de proteinas híbridas recombinantes capaces de generar anticuerpos neutralizantes em contra del veneno de alacranes MX2016002188A (2015) WO2017142388A2 (2017)

With B. Becerril Luján, J.N. Arredondo López, I.V. Gómez Ramírez, E.R. Rodríguez Rodríguez, L. Riaño Umbarila, G. Fernandez Taboada & T.C. Olamendi Portugal. Human recombinant antibodies family of variants that neutralize the venom of the main poisonous scorpions MX2015011378A (2015)

With I. Arenas Sosa, R. Rodríguez Hernandez, O. Walls Flores & G. Corzo Burguete. Actividad antimicrobiana “in vitro” de la combinación de péptidos antibiocidas de alacrán con antibioticos de uso común MX2015009505A (2015)

With E. Ortiz, S. Ramírez Carreto, J.M. Jimenez Vargas & G. Corzo. Péptidos del alacrán Vaejovis punctatus como antibioticos contra Mycobacterium tuberculosis y outras bacterias y levaduras patógenas MX359976B (2015)

With P.G. Gurrola Briones, S.P. Salas Castillo, C.V. Ferreira B., Z.S. Varga, G. Panyi & R. Gaspar. Vm23 and Vm24, two scorpion peptides that block human T-lymphocyte potassium channels (sub-type Kv1.3) with high selectivity and decrease the in vivo DTH-responses in rats (2012) Europe 07734817.5, US 12/599,978 CN200780053305.7 CA2686216 AU2007353147 Israel202113 Euroasia 200901530 Korea 10-2009-7026000 Brazil (?) Cuba 2009-0194 JP (?) India 7396/DELNP/2009 Singapore 20097395-8 South Africa (2009) PCT (2007)

With G. Corzo & F. Garcia. Uso de un péptido antibiotico proveniente del veneno del alacrán Centruroides suffusus suffusus y sus composiciones farmacéuticas obtenidas con antibióticos comerciales MX 343126B (2011)

With G. Corzo, G. Estrada, K. Hernandez & B.I. Garcia. Variantes recombinantes de una toxina del veneno del alacrán Centruroides sufussus sufussus para la producción de antivenenos contra el veneno de alacranes del género Centruroides (2011)

With A. Alagón C, G. Gurrola, E.V. Grishin, A.V. Lipkin & K.E. Volynski.Immunogen, anti-venom and vaccine against the venom of the Black Widow Spider US (?) EP00980069.9 CA2,397,731 (2002) MX (2010) PCT(2000) UNAM-MX (1999)

With G. Gurrola Briones, C.A. Hernandez Aponte & J. Silva Sanchez, Vejovina – un péptido antibiótico (2009)

With L. Riaño U & B. Becerril L. Human antibody variants that specifically recognize the toxin CN2 from Centruroides noxius scorpion venom US (2008 & 7,381,802 B2 2005) Mexico (2009)

With M. Corona, M.C. Garcia, N.A. Valdez, G. Gurrola & B. Becerril. Recombinant immunogens for the generation of antivenoms to the venom of scorpions of the genus Centruroides US (2008 & 2002) Mexico (2003)

With G.A. Corzo Burguete, G. Estrada Tapia, K. Hernandez-Salgado & B.I. Garcia Gómez. Inmunógenos recombinantes para la producción de antivenenos contra el veneno de alacranes del género Centruroides (2008)

With B.I. Garcia-Gómez & T.C. Olamendi-Portugal. Inmunógenos recombinantes para la producción de antivenenos contra el veneno de alacranes del género Parabuthus MX339085B (2008)

With F. Zamudio & A. Torres. Hadrurina – Un péptido antibiótico (2007)

With B. Becerril, F. Zamudio, B. Selisko, A. Ramírez & M.C. Garcia. Primary sequence and cDNA of insecticidally effective toxins from scorpions of the genus Centruroides US (2001) MX 2005&2002

With B. Becerril & A.F. Licea N. ADNc y fragmento Fab del anticuerpo BCF2 y su utilización em composiciones farmacéuticas neutralizantes de veneno de alacrán (2001)

With C. Noeske-Jungblut, W.D. Schleuning, A. Alagón, D.A. Cuevas-Aguirre, P. Donner, B. Haendler & U. Hechler. Thrombin inhibitor from protostomia saliva (Schering-US) 1999 US 5,876,971A (1992)

With C. Noeske-Jungblut, B. Haendler, J.R. Kraestzschmar, W.D. Schleuning, A. Alagón & D.A. Cuevas-Aguirre. Collagen-induced platelet aggration inhibitor (Schering US & Europe) 1998

With C. Noeske-Jungblut, W.D. Schleuning, A. Alagón, D.A. Cuevas-Aguirre, P. Donner, B. Haendler & U. Hechler. Clotting inhibitor made from protostomia saliva (Schering, Europe) 1997

With B. Becerril, M. Corona, F. Ingeborg, F. Zamudio, E.S. Calderón, P. Litton & B.M. Martin. Produção de peptideos de escorpiões Tityus serrulatus, Tityus bahiensis e Tityus stigmurus, e respectiva imunização de cavalo, visando a obtenção de soros antiescorpionicos Brazil (1995)

With G. Gurrola B., M.A.A. Bayón C & M. Sitges B. Procedimiento, diseño y síntesis para la obtención de péptidos sintéticos de estructura (Ax)N-(As)N-As, capaces de formar derivados beta-carbonilos para sustratos fluorogénicos de enzimas hidrolasas (1991)

With G. Gurrola B., M.A.A. Bayón C & M. Sitges B., Synthetic noxiustoxin related peptides (US 1990)

HONOURS

Doctor Honoris Causa, Hungarian Academy of Sciences (2005)

Redi Award, International Society of Toxicology

Genus Possaniella Brailovsky 1999 (Hemiptera)