Guofan Hong, Chinese molecular biologist (Ningbo, Zhejiang Province 24 December 1939 -
Made notable contributions to nucleotide sequencing technology working under supervision of Nobel prize Frederick Sanger (1970s)
Discovered that the diffusion of nucleic acids may be counteracted by electric force while moving in the gradient electric field (1978)
A method for sequencing single-stranded cloned DNA in both directions. Biosc. Reports 1:243-52, 1981
Established heat stable Bst DNA sequencing system (1987)
Improved the efficiency of nucleotide sequencing technology (1992)
Proposed a molecular model based on gene nodD by which the nodulation genes in Rhizobia were regulated (1994)
Designed a rapid and accurate BAC fingerprinting-anchoring strategy used in the construction of BAC-contig map of the rice genome (1997)
Methods
With S.H. Lee, S.C. Ge & S.X. Zhou. A novel low temperature PCR assured high-fidelity DNA amplification. Int. J. Mol. Sci. 14(6):12853-62, 2013
With S.T. Liu. Three minute G+A specific reaction for DNA sequencing. Anal. Biochem. 255(1):158-9, 1998
With Y.Y. Lu & F.D. Ni. Rapid method for identifying subclones bridging gaps between contiguous DNA sequences. Chin. J. Biotechnol. 5(3):157-60, 1989
A simple method for identification of gap-bridging subclones in DNA sequencing. Biosc. Reports 8(6):549-51, 1988
With M.D. Biggin & T.J. Gibson. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA 80(13):3963-5, 1983
A systemic DNA sequencing strategy. J. Mol. Biol. 158(3):539-49, 1982
Patents
With Y.J. Yang & J. Zhu. Low-temperature cycle extension of DNA with high polymerization (priming) specification. CA2449560A1 (2002) JP2003503756A (2002) AU2002324272A1 20030087237 (2003)
With W.H. Huang. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides 6165765 (1998) 6845909 (2000) 6818431 (2000) USPTO 20050089910 (2004)
With F. Zhai & W.H. Huang. A new DNA polymerase with proof-reading 3’-5’ exonuclease activity EP0810288A3 (1996) US08/642,684 (1996) JP8160402A (1996)
With F. Zhai. New bst-DNA polymerase with proof-reading 3’-5’ exonuclease activity JPH09140376A (1995)
With F. Zhai. Bst DNA polymerase with proof-reading 3’-5’ exonuclease activity / A DNA polymerase which is highly efficient and which can withstand high temperatures (HiFiBst) EP0712927 A2 (1995) CN94118990 (1994)
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