1 What is the difference between Biotechnology and genetic engineering?
2 What is the relationship between genetic engineering and recombinant DNA technology?
3 What is the principle behind recombinant DNA technology?
4 Why does recombinant DNA technology require the use of a vector instead of directly inserting the DNA into the target cell's genome?
5 What is the difference between plasmids and chromosomes in bacteria?
6 How do you think restriction enzymes work? Why can't restriction enzymes ensure they only cut out the target DNA fragment?
7 What is the importance of DNA ligase?
8 Restriction enzymes recognize specific base sequences before cutting. Would recognizing shorter or longer base sequences be more effective? Why?
9 Why might genetically modified (GM) foods be harmful to humans?
10 What is the principle behind gene therapy? What are its limitations? After treatment, will the patient's descendants also be free from hereditary diseases?
11 Why might genetically modified organisms (e.g., larger salmon) disrupt the original ecosystem?
12 What is the principle behind DNA fingerprinting? What are its limitations?
13 What is the principle of gel electrophoresis? Why is it important to monitor time when using this method?
14 When using gel electrophoresis, why do some DNA fragments move faster than others?