Masters Applied Projects

Project 15

Exploration of Buffer Capacity and pH on PEDGA/QT Gelation Characteristics

Student:
Priscilla Han

Mentors:

Dr. Olivia Burnsed, PhD – Arizona State University, SBHSE
Dr. Brent Vernon, PhD - Arizona State University, SBHSE
Dr. Jessica Weaver, PhD - Arizona State University, SBHSE

YouTube Link:
View the video link below before joining the zoom meeting

Zoom Link:
https://asu.zoom.us/j/8937698572


Abstract
Aneurysms are characterized by bulging in the blood vessel wall due to structural failure. When left untreated, aneurysm rupture results in strokes. Poly(ethylene glycol) diacrylate (PEGDA) and pentaerythritol-tetrakis (3-mercaptopropionate) (QT) can be mixed and injected into the aneurysm space to decrease intravenous pressure within the blood vessel. While previous studies have shown that temperature, pH, and mixing time are key factors that control PEGDA/QT gelation characteristics, the effect of buffer capacity has not been explored. The scope of this preliminary study focuses on comparing phosphate-buffered saline (PBS) with a buffer salt concentration of 11.8 mM and a phosphate buffer (PB) with a buffer salt concentration of 99.88 mM using in vitro benchtop testing. In addition to assessing the effect of buffer capacity on gelation time, we will also characterize the gel’s swelling and degradation trends. The results from these studies will help optimize the PEGDA/QT formulation and utilization process and increase the reliability of its clinical application.