Embedded Files
Sustainable Agriculture | Plant-Microbe Interactions
Sustainable Agriculture | Plant-Microbe Interactions
A. N. M. Shahriar Zawad1, Tanim Jabid Hossain1.2*, Md. Mahfuz Ahmed1, Nadia Islam1, Imteaj Uddin Chowdhury1, Mohammad Razuanul Hoque1
Summary: Plant growth-promoting rhizobacteria (PGPR) enhance plant growth, biomass production, and environmental adaptability, offering a sustainable, eco-friendly solution to boost crop productivity and agricultural sustainability. This study evaluated the impact of six PGPR isolates – Bacillus cereus PGPR-13, Acinetobacter oleivorans PGPR-14, Staphylococcus epidermidis PGPR-15, Pantoea dispersa PGPR-24, Serratia nematodiphila PGPR-27, and Pantoea anthophila PGPR-34 – and their consortium, on Chrysanthemum growth under pot and field conditions. The PGPR strains, previously isolated from chrysanthemum rhizosphere and confirmed for in vitro plant growth-promoting activities, significantly enhanced key growth parameters, including stem length, root length, stem diameter, and biomass accumulation. Among individual treatments, A. oleivorans (PGPR-14) achieved a remarkable 114% increase in stem length during pot trials, while the consortium exhibited the highest stem diameter (1.09 cm) and dry biomass (33.03 g). Field trials confirmed these findings, with consortium-treated plants attaining superior growth metrics, including a stem length of 75.33 cm, root length of 31.73 cm, fresh biomass of 512 g, and dry biomass of 284.33 g, outperforming most individual strains. S. epidermidis (PGPR-15) and S. nematodiphila (PGPR-27) also demonstrated notable biomass enhancements, with mean fresh weights exceeding 390 g in field trials. These results highlight the synergistic effects of the PGPR consortium and the exceptional growth-promoting potential of individual strains such as A. oleivorans, S. epidermidis, and P. dispersa. The study underscores the promise of PGPR as sustainable biofertilizers to enhance chrysanthemum growth, offering an effective and ecofriendly solution to reduce dependence on chemical inputs. Future research should focus on optimizing microbial consortia, elucidating their functional mechanisms, and expanding their applications to enhance crop productivity and sustainability across diverse agricultural systems.
Plant-Microbe Interactions | Genome Sequence Analysis
Plant-Microbe Interactions | Genome Sequence Analysis
Tanim Jabid Hossain*
Summary: Plant growth-promoting rhizobacteria (PGPR) are integral to sustainable agriculture, enhancing plant growth, nutrient availability, and soil health. The genome analysis of Pantoea dispersa PGPR-24, a rhizobacterium isolated from the chrysanthemum rhizosphere, reveals its extensive potential as a PGPR supported by diverse genetic pathways linked to nutrient mobilization, plant growth promotion, and stress adaptation. The 4.746 Mb genome, with 99.37% completeness and 4,411 coding sequences, encodes key genes for phosphate solubilization, siderophore-mediated iron acquisition, sulfate assimilation, and ammonia assimilation, highlighting its role in nutrient cycling and bioavailability. Genes associated with auxin and cytokinin biosynthesis suggest its potential to produce phytohormones that regulate root architecture, enhance nutrient uptake, and support plant development. Additionally, the genome encodes biosynthetic pathways for volatile organic compounds (VOCs), including acetoin and 2,3-butanediol, which are known stimulate root elongation, improve stress tolerance, and activate plant defense responses. Furthermore, the genome features compounds with antimicrobial and protective properties, such as siderophores, carotenoids and exopolysaccharides, which contribute to pathogen suppression, biofilm formation and enhanced rhizosphere colonization. Genes supporting motility, chemotaxis, and adhesion further strengthen potential for efficient colonization and plant-microbe interactions. Stress-response mechanisms, including pathways for osmoregulation, oxidative and periplasmic stress tolerance, and starvation resistance, equip the strain to thrive in diverse environmental conditions. These genomic insights, complemented by its reported in vitro plant growth-promoting traits, not only position P. dispersa PGPR-24 as a highly versatile rhizobacterium for sustainable agriculture but also offer a valuable genetic framework for advancing our understanding of PGPR-mediated plant growth promotion and stress resilience.
Computational Drug Discovery | Bioactive Compound
Computational Drug Discovery | Bioactive Compound
Tanjilur Rahman, Mohammed Sajjad Hossain Bappi,Tanim Jabid Hossain*
Summary: Dengue virus (DENV) and Zika virus (ZIKV), members of the Flaviviridae family transmitted by Aedes mosquitoes, continue to pose significant global public health challenges. Despite extensive research, effective treatments for these viruses remain elusive, highlighting the urgent need for new and efficient antiviral therapies. This study explores prodigiosin, a microbial tripyrrole pigment, as a potential antiviral agent against DENV and ZIKV through comprehensive computational analyses. Prodigiosin exhibited favorable drug-likeness properties, meeting Lipinski's rule of five and demonstrating optimal physicochemical and pharmacokinetic characteristics according to Ghose's, Veber's, Egan’s and Muegge's filters, essential for oral bioavailability. ADMET profiling further revealed high intestinal absorption, minimal risk for drug-drug interactions, and a low toxicity profile, with no observed AMES toxicity, hepatotoxicity, or skin sensitization. Molecular docking studies showed prodigiosin’s strong binding affinities to the NS5 methyltransferases of both viruses, suggesting disruption of the viral replication process. Notably, prodigiosin's binding affinities were comparable to those of chloroquine and ribavirin 5'-triphosphate, known inhibitors of ZIKV and DENV, respectively. Molecular dynamics simulations further supported these findings, demonstrating stable and specific interactions between prodigiosin and NS5 MTases, with low root mean square deviation values. Additional analyses, including root mean square fluctuation, radius of gyration, and solvent-accessible surface area, confirmed the compactness and stability of the complexes. These promising results suggest that prodigiosin could serve as a broad-spectrum antiviral agent, warranting further experimental validation and continued investigation for therapeutic development against flaviviral infections.
Traditional Fermented Beverages| Probiotic Benefits
Traditional Fermented Beverages| Probiotic Benefits
Tanim Jabid Hossain*, Md. Sajib Khan, Jannatul Ferdouse
Summary: Fermented and dairy beverages, enriched with probiotics, stand as integral components of Bangladesh's culinary heritage. This article explores the diverse landscape of these beverages, emphasizing their significance as potential reservoirs of probiotic lactic acid bacteria. From the iconic fermented beverage date palm sap to an array of dairy staples like milk, borhani, and laban, each beverage presents a unique microbial profile. Studies isolating strains within these drinks underscore their probiotic potential, exhibiting multifaceted benefits such as antimicrobial efficacy, antioxidant capabilities, a cholesterol-lowering effect, and exopolysaccharide production. In vitro probiotic assessments further unveil the strains' ability to withstand gastric conditions and colonize the gastrointestinal tract. The exploration extends to genomic dimensions, unraveling the genetic basis of promising probiotic strains and offering insights into their mechanisms of action. This includes the identification of genes encoding key bioactive metabolites, antimicrobial peptides, nutraceuticals, and flavor compounds. Importantly, while this research is rooted in the context of Bangladesh's culinary heritage, the implications of probiotic lactic acid bacteria extend globally, enriching our understanding of probiotics' impact on health, offering potential benefits to populations worldwide. However, this burgeoning field faces certain limitations, necessitating further research to bridge gaps in understanding the exact mechanisms for probiotic functionalities, specific health effects, and unexplored probiotic properties. The intricate interplay between the beverages and lactic acid bacteria opens avenues for innovative applications beyond tradition, leveraging the full potential of these probiotic-rich beverages for functional food development, health-focused interventions, and potentially advantageous contributions to food preservation. Importantly, while this research is rooted in Bangladesh's culinary tradition, the implications of probiotics extend globally, enriching our understanding of their health benefits and potentially influencing wellness worldwide.
Environmental Engineering | Bioremediation
Environmental Engineering | Bioremediation
Biosurfactant-Producing Bacteria: Key Insights into Isolation, Characterization, Production, and Applications in Environmental Remediation and Microbial Enhanced Oil Recovery (MEOR)
Biosurfactant-Producing Bacteria: Key Insights into Isolation, Characterization, Production, and Applications in Environmental Remediation and Microbial Enhanced Oil Recovery (MEOR)
Tanim Jabid Hossain*, Md. Sajib Khan
Summary: Biosurfactants, amphiphilic molecules produced by diverse microorganisms, play a pivotal role in environmental remediation and microbial enhanced oil recovery (MEOR) due to their unique properties. These molecules are identified through rigorous screening processes following the isolation and characterization of biosurfactant-producing bacteria from various environments, including soil, marine habitats, extreme environments, industrial settings, and oil reservoir. Methods such as drop collapse test, oil displacement method, surface tension measurement, emulsification assay, hemolysis assay, and Thin layer chromatography (TLC) are commonly employed to determine the biosurfactant producing capacity of these strains. For industrial applications, scaling up production often requires optimizing culture conditions, bioprocess engineering, and fermentation parameters. In environmental bioremediation, biosurfactants enhance the biodegradation of hydrophobic pollutants, facilitating the cleanup of oil-contaminated soils and waters. Their ability to solubilize and emulsify hydrocarbons makes them effective in mitigating the environmental impacts of oil spills and industrial effluents. Additionally, biosurfactants are significant in MEOR strategies as they improve oil recovery efficiency by reducing interfacial tension, altering wettability, and mobilizing trapped oil fractions in reservoirs. Their biodegradability, low toxicity, and robustness in harsh conditions make them sustainable alternatives to chemical surfactants in petroleum extraction processes. In addition, biosurfactants have diverse applications across multiple sectors, including their use as biopesticides and soil conditioners in agriculture, emulsifiers in the food industry, and drug delivery systems and skincare formulations in healthcare. This review synthesizes recent advancements in biosurfactant research, emphasizing their diverse habitats, screening strategies, production, and applications in sustainable environmental remediation and MEOR. Future research should explore biosurfactant producing strains having high efficacy and innovative applications of biosurfactants to address evolving environmental and industrial challenges.
Bacterial community diversity | 16S metagenomics
Bacterial community diversity | 16S metagenomics
Tanim Jabid Hossain*, Shahadat Hossain, Iqbal Hossain Nafiz, Raihanul Islam, Md. Sajib Khan
Summary: The microbial community in fermented foods significantly impacts their flavor and quality, and metagenomic analysis serves as a valuable tool for comprehending the composition and diversity of the microbial community within fermented foods. Date palm sap, also known as date palm juice, is a nutritionally cherished beverage anticipated to host a diverse microbial community capable of influencing its quality, safety, and potential health benefits. Nevertheless, research on microbial community and diversity in date palm sap remains limited. In this study, we conducted the first metagenomic analysis of this beverage using high-throughput sequencing targeting the V3-V4 region of the 16S rRNA gene to unveil its intricate bacterial composition. Proteobacteria and Firmicutes emerged as the predominant bacterial phyla in all samples, collectively constituting over 85% of the microbiome on average. At the genus level, we observed higher diversity, with the lactic acid bacteria groups such as Leuconostoc and Lactobacillus exhibiting high relative abundance, potentially playing a significant role in sap fermentation. Additionally, we identified other bacterial groups including Zymomonas, Acinetobacter, Fructobacillus, Lactococcus, Pseudomonas, Sphingomonas, Ralstonia, and others, commonly present in the samples. Furthermore, functional predictions of the microbial communities indicated a primary association with metabolic functions, genetic information processing, environmental information processing, and cellular processes. Their proteins were predominantly linked to membrane transport, amino acid metabolism, and carbohydrate metabolism. While specific correlations require further investigation, these findings offer insights into the complex microbial ecology of date palm sap. Future studies should prioritize isolating key bacterial species to uncover and harness their precise contributions to specific juice characteristics.
Antimicrobial Detection
Antimicrobial Detection
Tanim Jabid Hossain*
Summary: Infectious diseases pose a formidable global challenge, with the escalating threat of antimicrobial resistance complicating their treatment. In response, researchers are actively exploring natural and synthetic antimicrobial compounds as potential solutions. This pursuit of novel antimicrobial agents highlights the critical role of the methods used for screening and evaluating antimicrobial activity - a vital step in the discovery and characterization of effective antimicrobial compounds. While conventional techniques like well diffusion, disk diffusion and broth dilution methods are widely used in antimicrobial assays, they may face limitations in reproducibility and speed. Additionally, a variety of antimicrobial testing methods including cross-streaking, poisoned food, co-culture, time-kill kinetics, resazurin assay, bioautography, and more, are routinely employed in antimicrobial evaluation, expanding the range of assessment tools. Advanced techniques such as flow cytometry, impedance analysis and bioluminescent techniques may provide rapid and sensitive results, offering a deeper insight into the impact of antimicrobials on cellular integrity and viability, but their higher cost and limited accessibility in some laboratory settings can pose challenges. This article presents a comprehensive overview of the in-vitro and in-situ assays designed to characterize antimicrobial activity in natural and synthetic compounds, discussing the underlying principle, protocol, advantages, and limitations of each method. The primary objective is to enhance the understanding of available methods for evaluating antimicrobial agents in the ongoing battle against infectious diseases. By selecting the appropriate antimicrobial assay method, researchers can determine the most suitable conditions for assessment, and streamline the identification of effective antimicrobial agents.
Environmental Microbiology | Bioremediation
Environmental Microbiology | Bioremediation
Isolation of Biosurfactant Producing Bacteria from Oil-Spilled Soil and Characterization of Their Secreted Biosurfactants in Pathogen-Inhibition and Oil-Emulsification
Isolation of Biosurfactant Producing Bacteria from Oil-Spilled Soil and Characterization of Their Secreted Biosurfactants in Pathogen-Inhibition and Oil-Emulsification
Mohammad Zobaer, Ferdausi Ali, Md Anwar*, Mohammed Sajjad Hossain Bappi, Takia Binte Bakar, Tanim Jabid Hossain*
Summary: Since phosphorus remains mostly in an unutilizable form in soil, the phosphate solubilizing bacteria (PSB) can be employed to increase availability of soluble usable phosphorus in the rhizosphere. Consequently, in this study three highly efficient phosphate solubilizing strains were screened out from the rhizospheric soil of BRRI-28 rice variety and characterized for plant growth promoting and abiotic stress tolerance properties. 16S rRNA gene sequence analysis identified the three isolates as Enterobacter and Klebsiella strains. They exhibited multiple plant growth promoting traits including auxin secretion, zinc solubilization, or ammonia production. The phosphate solubilizing and zinc solubilizing indices of the isolates were determined. Lipolytic activity was found to be the most common hydrolytic activity detected in all of the isolates. The PSB were further evaluated for their tolerance to different degrees of salinity (3 to 11% NaCl), drought (10 to 50% PEG-6000) and temperatures (20°C, 30°C and 37°C). The isolates could tolerate salinity stress up to 7% NaCl, drought stress up to 30% PEG-6000, and grew at all the tested temperatures with maximum growth detected at 30℃ or 37℃. Therefore, the phosphate solubilizing isolates can be considered candidates as microbial inoculants for plant growth enhancement and agricultural productivity under stress conditions.
Related:
Related:
Google Sites
Report abuse