Reference

Cytochrome P450 HomepageNelson, DR (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfoldAutoDock VinaO. Trott, A. J. Olson, AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading, Journal of Computational Chemistry 31 (2010) 455-461

D. R. Nelson's

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm,  recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output 

CYP152 (Peroxygensase)

>CYP152A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNEQIPHDKS LDNSLTLLKE GYLFIKNRTE RYNSDLFQAR LLGKNFICMT 00500051 GAEAAKVFYD TDRFQRQNAL PKRVQKSLFG VNAIQGMDGS AHIHRKMLFL 01000101 SLMTPPHQKR LAELMTEEWK AAVTRWEKAD EVVLFEEAKE ILCRVACYWA 01500151 GVPLKETEVK ERADDFIDMV DAFGAVGPRH WKGRRARPRA EEWIEVMIED 02000201 ARAGLLKTTS GTALHEMAFH TQEDGSQLDS RMAAIELINV LRPIVAISYF 02500251 LVFSALALHE HPKYKEWLRS GNSREREMFV QEVRRYYPFG PFLGALVKKD 03000301 FVWNNCEFKK GTSVLLDLYG TNHDPRLWDH PDEFRPERFA EREENLFDMI 03500351 PQGGGHAEKG HRCPGEGITI EVMKASLDFL VHQIEYDVPE QSLHYSLARM 04000401 PSLPESGFVM SGIRRKS

Heme Docked

PDBj 2.1Å

CYP152A1 (P450BSβ)WT
>CYP152B1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPKTPHTKGP DETLSLLADP YRFISRQCQR LGANAFESRF LLKKTNCLKG 00500051 AKAAEIFYDT TRFEREGAMP VAIQKTLLGQ GGVQGLDGET HRHRKQMFMG 01000101 LMTPERVRAL AQLFEAEWRR AVPGWTRKGE IVFYDELHEP LTRAVCAWAG 01500151 VPLPDDEAGN RAGELRALFD AAGSASPRHL WSRLARRRVD AWAKRIIEGI 02000201 RAGSIGSGSG TAAYAIAWHR DRHDDLLSPH VAAVELVNVL RPTVAIAVYI 02500251 TFVAHALQTC SGIRAALVQQ PDYAELFVQE VRRFYPFFPA VVARASQDFE 03000301 WEGMAFPEGR QVVLDLYGSN HDAATWADPQ EFRPERFRAW DEDSFNFIPQ 03500351 GGGDHYLGHR CPGEWIVLAI MKVAAHLLVN AMRYDVPDQD LSIDFARLPA 04000401 LPKSGFVMRN VHIGG

Heme Docked

PDBj 1.7Å

CYP152B1 (P450SPα) WT
>CYP152C1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTDEGRRPE EPGTPASLRE MPRDPRIDAS MALMSEGYRF VSNLCDRMDS 00500051 DAVATRLRLR EVVCLRGSAA ARLLYGAEGL TRVGAMPSTV LHLLQDKGSV 01000101 QQLEGPAHRH RKALFLSICM DPARVEALVS EMRLAWRERL PAWEAEGRIV 01500151 LQQEAARLLT RAGCRWAGVA HQPEAQLADE IFDMIDKAGS VGPRNWLAQM 02000201 RRAGTEKRLR TLVEEVRAGE VVPEAATALH AIAFHREEDG TLLDPSVAAV 02500251 ELLNLLRPIV AVGRYITFAA LALHRETTWR ELFRSGNLEL AGDFAEEVRR 03000301 ASPFFPFTAA VTTRPITWEG YDFPEGQWLL LDLYGTTHDP RHFPEPTRFR 03500351 AERMLSWTGQ DEAFIPQGAG DVARTHRCPG EMITVELMKE AIRLLCCEMD 04000401 YEVPAQDLGV RLNRMPAQPR SGMILSAISR RAGTEASRNG 

Heme Docked

PDBj

>CYP152D1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGMMHSRRTP LVDSSLSLLV RGYTWLPDRR RRTTGPLVRA RLTGRPAVAL 00500051 RGPEAVRFFY DERHVERASA LPGPVLGTLF GHGAVHTLDG PPHRVRKDLF 01000101 LSLLTGPKAV AGLVDHVTRA WDAAEASWPH RPSVVLFDEA SRVLTLGVCR 01500151 WAGIPLHDRD ADGAAPAADL VAMVDGFATP GPRHWRARRA RGRREAWLAR 02000201 LVSDVREGAA TAPAGSALDV VARHEDADGL PLDPHTAAVE LLNVIRPTVA 02500251 VCWFVAYAGH ALHLRPDVRE RLAEADPEYA VAFAHELRRF YPFAPFVGGR 03000301 AVTDLEWRGE PIPAGALVLL DLYGQNHDPG QWDLPYTFEP QRFLERPPGR 03500351 DDLVPQGGGD RATGHRCPGE DVTVALLRTL GPRLARLEYA VPPQDLRIPL 04000401 TRMPARVRSG FVMESVRVPE RVRVAGRAAG 

Heme Docked

PDBj

>CYP152E1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIKKIPQDKW IDSSLALIFD GYDFISKRCK RYQSNIFQTR LMLEKTICFK 00500051 GEEAAKIFYD KTKFTRKNAA PDRVKKTLFG EKGIQGMDGD AHLHRKQMFM 01000101 SLMSKERIQE VGTLTQKQWH NYAKKWERMD TIVLFEEARE VLCRVACTWA 01500151 GVPLEESEVK LRSQQFEAMI AGSGVVGPKH WQGRKARKEN EQWIETIIEK 02000201 VRNHQLEVPE ISATYIFANH RDLSGELLDK RVAAVELINV LRPIVAIDRY 02500251 ITFGALALHE HPECRPKLLE SEDYLRAFVE EIRRFYPFFP FASARVRQDF 03000301 DWQGYHFPEG TRVLLDLYGT NRDPQSWDKH DVFYPERFLN FSELDSFKFI 03500351 PQGGGDYHTN HRCPGEWITI ELLKVTMTFL IQSINYNVPK QDLRINHLKM 04000401 PTVPASGFVI KNVNLC

Heme Docked

PDBj

>CYP152G1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTQMPNNTFF DDTFALARDP YRFISKQCER HGSDAFETRL LLQKVICLRG 00500051 EEAAKAFYDT SRFSRVSVAP PRIAKTLFGR GGVQGMDGKA HRHRKMMFMS 01000101 LLTEDKIGGL TQLASDIWNR RILQWSRMDQ VVLYRQAQEV LTEAVCQWAG 01500151 VPLDPADVDR RTSELAALFD YAGSIGPKHW WARIARKRNE AWLRGIIEQI 02000201 RAGTYQPLED TAAFIVANHR DLDGNLLDAQ IAAVELNNIL RPTVAVSAYI 02500251 VQCAHALHEH PVIRQQLADG SPDDFQCFVQ EVRRFYPYFP FAGAKVKESF 03000301 EWQGYHFPSG RKVLLDLYGT NHDPRLWDEP ESFRPSRFHA WDGNPFTLIS 03500351 QGGGDHYQNH RCPGEWIAIE QMKAATDLLV NRCSYEIPEQ DLQIDMDRLP 04000401 AIPADHFVMT NIQLLREVR

Heme Docked

PDBj

>CYP152H1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTETEIPRD HGLDHSLALL REGYVFIRNR CRKLQTDIFQ TRLLAQNVIC 00500051 MTGPEAAELF YDEERFVRKG AAPKRVQKTL FGQNGVQGLD GARHRHRKQM 01000101 FMKLMSPERL QVIVDLTKKQ WLETSKKWMQ RDQVVLFDEV QEMLCQIACE 01500151 WAGISITGQE VKNRANDLGK MVDAFGAIGP RHWQGRIARV RSEQWAGQII 02000201 EHVRKGEMNP PESSAAYTMA WYRESNGRIL DTQIAAVELL NILRPIVAIA 02500251 TYITFGAHAL HEHPECMDNL QSGKGDVRPF VQEVRRYYPF TPFVGARVRR 03000301 EFAWKQCPFT RGTLVLLDVY GTHHDPRLWD RPDEFRPDRF HHWAGGLFDF 03500351 IPQGGGDRDH GHRCAGEWLT LKVMEASLDF LANQITYHVP VQDLSINLSR 04000401 IPTLPASRLI ISNVRQRNN

Heme Docked

PDBj

>CYP152J1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVKPLPKEEG LDHTIAWMNE GYLFLSNRMN QFNSDIIETK ILGKKCIAMR 00500051 GPEAAELFYD SSRFIRYGAL PKKAASVIIG KNSVFHLDDG QHHKRKQMLL 01000101 NVLNDEQQIQ LEQISKNQWE IAIEQWKQKD EIVLLDELEQ LFTITVCNWI 01500151 GIPLYAKNVS LRTKDIRSML TAFTNKGTNY FQGNQAKKRA LKWISYIIRQ 02000201 VRTGRIHPKE KSPLYQIAWY EDSNGKLLSI PNAAEEVINI IRPFVAITRY 02500251 ACFSQLAILE NKQVKHRLHK DSQYVDWFIQ EVKRFYPAVP FVVAKARESF 03000301 IWKDIYFPKN RLVLLDLYGS NHHQDTWEEP EKFVPERFQF LDKKDRYKLI 03500351 PQGAGDLEFG HRCAGEGITT QMMRISLEYL LRLDYDVPDQ DLNYKLNQVP 04000401 AQPSSKIRLQ NIRIKE

Heme Docked

PDBj

>CYP152K1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MASEKEIPKE AGIDNSVAVL REGYLYGTNR AQAFQSDLFK TRLLGQEAIC 00500051 IRGEEAAELF YDNEKFKRAG AAPKRVLKTL FGKGGVQALD GEAHHHRKAM 01000101 FMSLMSKETL EDIRAIASKQ WERAAQRWQN QSKVVLYEEA QEVMCRTAFE 01500151 WAGIPVEEDE VKDKAEWFRA MIEAPAAIGP AHWKGRRSRG KAESWVKELV 02000201 EDVRTGKRQA AEGKTLHTMS FHRELNGEWM DAQTAAVEII NILRPIVAIS 02500251 IYICFTALAV HQHPQEREKL GSDEEKLQHF VQEVRRFYPF FPFVPARPIK 03000301 DFTWNGYPFK KGTLVLLDLY GTNHHPKLWD NPEQFRPYRF ATWNKSPFAF 03500351 IPQGGGDRNV GHRCAGEWIT IEIMKESLDF LVNRIDYTVP EQDLRFQFNE 04000401 MPALPHSHFV MTNVRFQ

Heme Docked

PDBj

>CYP152K6 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSNINQMPRE EGIDSTWRLM EEGYMYILNR RHSFNSDIFE TRLLGKKAIC 00500051 MGGKEAAEIF YDTEKFKRKD AAPNRVVQTL FGKNGVQALD GQTHKHRKEM 01000101 FMSIMSPDEL EKLTDITKKQ WEIAVDKWEQ MDKVILYEEA KEIMCRTACQ 01500151 WAGVPVQENE VKRLTKNLGA MFESAAAVGL KHWLGRHARN YEEIWIEELI 02000201 DRVRDGKVNP PENTTLHKFS WYRDLEGNLL DTETAAVEVI NILRPIVAIA 02500251 IFINFIALAL HHYPEEKEKL KSGDKKYSQM FVQEVRRFYP FFPFVVALVK 03000301 KDFTWKGYKF EEGTLTLLDL YGTNHDPEIW KNPDVFSPDR FAKWEGSPFS 03500351 FIPQGGGDYF MGHRCAGEWV TIEVMKVSLD YLTNRMDYEV PDQDLSFSMA 04000401 SMPSIPHSKV VIKNVKKRI

Heme Docked

PDBj 1.3Å

CYP152K6
>CYP152L1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MATLKRDKGL DNTLKVLKQG YLYTTNQRNR LNTSVFQTKA LGGKPFVVVT 00500051 GKEGAEMFYN NDVVQREGML PKRIVNTLFG KGAIHTVDGK KHVDRKALFM 01000101 SLMTEGNLNY VRELTRTLWH ANTQRMESMD EVNIYRESIV LLTKVGTRWA 01500151 GVQAPPEDIE RIATDMDIMI DSFRALGGAF KGYKASKEAR RRVEDWLEEQ 02000201 IIETRKGNIH PPEGTALYEF AHWEDYLGNP MDSRTCAIDL MNTFRPLIAI 02500251 NRFVSFGLHA MNENPITREK IKSEPDYAYK FAQEVRRYYP FVPFLPGKAK 03000301 VDIDFQGVTI PAGVGLALDV YGTTHDESLW DDPNEFRPER FETWDGSPFD 03500351 LIPQGGGDYW TNHRCAGEWI TVIIMEETMK YFAEKITYDV PEQDLEVDLN 04000401 SIPGYVKSGF VIKNVREVVD RT

Heme Docked

PDBj

>CYP152M1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKEVPVVDIK ITDLKKLYQK GYNMLEELRH EADAPVVKAK IFNKEAITIY 00500051 GSSAAKVFYD PRNFKRKGAM PKLVLKTLFG QGGVQTLDGA AHHHRKNIFM 01000101 DLMTPERMED YHRILDKNLT QALEAQHGQF ELFDLSKMVF FTSICEWAGI 01500151 NLSAISKDEV EKLAEYQISM ISGTFTSPID HIKGVENRKK SEKWAQGLIE 02000201 EARQNPVAGK ENVALYAFAN ATDLDGQLLP LEVAAVELLN IIRPTVALTV 02500251 WAALMGHALF SRPDLYQQLK NDFSTLQDPF IQEMRRYYPF FPMLPAISLK 03000301 EVEVDGYRIP EGSWVILDLY GTDHDERTVE APDSFMIKRY VGKAKDISYK 03500351 EEYEMIAQGG GNFRQMHRCA GEWITLHSLR VFSDQLVNKF EFSVPEQDWT 04000401 IPFNQFPTYP NSRALLYKN

Heme Docked

PDBj

>CYP152N1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGKVIPKQEG LDHSVDFLRE GYLFVANRRK SFQSNIFESR LLGERVICLG 00500051 GEEAAEVFYD ANKFTRQDAA PKRLLKTLFG EGGVQTLDGS EHTHRKQMFM 01000101 SLMTKENIDR LLRLTYREWN QIERMGEEIV LYDIAQEVLM KAVCEWSGVP 01500151 LAKEEVGKRT EEMRLLFESG TSLGPTYLQG RKARSSAEVW IRQMVKEVRS 02000201 NRLLPNEHTA LYEFSWHRDE SGELLPEEVV AVEVLNILRP TVAISVYVLF 02500251 TVLALHQFPD VKEQVERGEV SKTEFVQEVR RFYPFFPVAA ARVKTDFEWD 03000301 GYAFPEGTLT LLDLYGTNHD VSIWTEPDRF DPSRFKDWKE SPFNFIPQGG 03500351 GDVDFGHRCA GEHVTIAILA QVIELFTKEY AYTVPPQDLS YSFVDMPSLP 04000401 KSKLRLTHLT RNQ

Heme Docked

PDBj 2.4Å

CYP152N1
>CYP152P1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPAAIATHRF RKARTLPREP APDSTLALLR EGYGFIRNRC RRHDSDLFAA 00500051 RLLLSPVICM SGAEAARHFY DGHRFTRRHA LPPTSFALIQ DHGSVMVLDG 01000101 AAHLARKAMF LSLVGEEALQ RLAGLAERHW REAVSGWARK DTVVLLDEAH 01500151 RVLTAAVCEW VGLPLGPTEV DARAREFAAM IDGTGAVGPR NWRGHLYRAR 02000201 TERWVRKVID EIRSGRRDVP PGAARTIAEH QDADGQRLDR TVAGVELINV 02500251 LRPTVANARY IVFAAMALHD HPHQRAALAD GGEAAERFTD EVRRFYPFIP 03000301 FIGGRVRAPF HFGGHDFREG EWVLMDLYGT NRDPRLWHEP ERFDPDRFAR 03500351 ETIDPFNMVS HGAGSARDGH RCPGEGITRI LLRTLSRQLA ATRYTVPPQD 04000401 LTLDLAHVPA RPRSGFVMRA VHAP

Heme Docked

PDBj

>CYP152Q1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVQLPDSSL ALLREGYTFI SSRCDRLGTD LFRTTLILRP VVCLRGAEAA 00500051 EFFYGGGRFG RKAAMPRSAQ HLLQDAGSVQ SLQGSAHRRR KQLFLDLMTN 01000101 ESVERLGRAF DTEWHSARER WQDSGEVVLH DEVRRVLTAT ACHWAGVSAD 01500151 RATVSRRARE LSLMIDKAGA VGPVNWYARW RRRSTEKWAG ECISSIRRNG 02000201 PAAASGSPAA VIAFHTDEHG NPLSAEVAAV ELLNLLRPIV AVSRFMVFAA 02500251 VALQQHPEWN DIVRKGQDAD LDCFAQEVRR YYPFFPFVGG TARETLEWKG 03000301 HTFKTGQWAL FDLYGTNHDG RLWKDPESFN PARFRVWRPD PHTLVPQGAG 03500351 DAAVGHRCPG EDITVDLIRR ATRALAAESG ISVPAQDLSI DLTRMPALPR 04000401 SGFILSVGRA SD

Heme Docked

PDBj

>CYP152R1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDIPRDDHL ESSLALLGEG YPFIRDRCQR LHSNVFQARL LMQNTICLSG 00500051 EQAARLFYDE RHFQRAQAMP RMLKKTLLGQ GGVQGLDGEA HRHRKRMFLQ 01000101 LLDAAAVDEL VGLTERSWRQ AIGQWQAQGE VQLLGELQML LTDSVCRWAG 01500151 VPLPSAEREL RRDQLAQMID GAGGIGPRHL AARKARREAE AWTQHLIEQV 02000201 RAGELQGDPT RALMVVAHHR DLDGKPLDSR IAAVELLNLL RPTVAVAYFI 02500251 TYAALELLAH PHWCERLRAE DELLEPFAQE VRRLHAFFPF TAARVRDGFD 03000301 WQGHHFPAGT RVMLDLWGTN REASRWTDPE AFQPERFVDW PGDAFGFVTQ 03500351 GGGDPAQGHR CPGERLAIEL LKLALRMLTR EMDYAVPAQD LRIDLSRMPA 04000401 KPESGLLISD VRPRAS

Heme Docked

PDBj

>CYP152S1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MARPPLESTY ALWKKGYVFI DSTCRALGVK AFRTRLMMRP AICMQGEAAA 00500051 RLFYDNEKFM RAGAMPARAK KTLVGEGGVQ GMDGHAHRHR KQMFTRELMS 01000101 PERIEHLVCL HRGHWKEAIQ AWKPGSEIVL FREAQKLLCK SVCAWAGVPL 01500151 TGHELDRRAR EFGHMIDSGA KLGPHHWRGR WARKSCERWL GQLIEDIRHY 02000201 GEQGQAGSIL QSIAWHRDEH GQRLPLQVAA VELINILRPT VAIAQLAVFC 02500251 ALALHSFPAC RQKLQQGDED YLGWFVLEVR RFYPFFPFLA AVVREDFRWQ 03000301 NLEFKRGQRV LLDIYGTHRD PALWQDPAQF IPERFKDWDG NSFGLIANGG 03500351 ATYEYHHRCP GEWITIALMK AITRMLVHEI EYTVPAQDLR LDLSRIPALP 04000401 ESGFIIQPVI TPRYLEKKHA NS

Heme Docked

PDBj

CYP152A1_Consensus

>Consensus/1-1142 Percentage Identity Consensus MNDHNAHMKPVSSIFTCVARHYLREHRSGRCEVCACAGTAAALLLAGAALVSGPGPGPRRKPRLLLLAPLVGACGVRFTCCCVHRAQRARHHMEECSKMPSDFHGPRWPPRADDRMLRMASPQAMTMHASRRPSTTDHPVAARLSAGVFGHAAQRLRDDAADAMMSMMEQIPRDKGLDSTLALLRSEGGYLFIPNRCRRYGSPGAQGKMGHGESAQTGMGTADVFETRLLGGQKAICLRGEEAARLFYDEQERFQRKRGAAAPKRVQKTLFGEGGVQTLDGEAHRHRKRAMFMSLMTTAPPEPGRPRVLARLAELVAEEWRAAVPRWEGRGPGRERDGDEEVVLFVLFDEAKEVLTRAVCRWAWAGVPLKESDAGVHPDGEHLDDEHRPDDEARAPADAHPDEEVKVERARDLVAMVDGFGGAVVGGAGPRHWFRGARRARRRAEKWIAGLIEDAVRRAGKLEQPAGARPHAWPAGGAAPAGFPAGPATAPEGSALHTFAEHREPDGYVVAFHHRDGNDGNGEDLDGELTAEPLDPRTAAVELLNVLRPTVAVSRFITFAALALHEHPEGWREKLRSGDGSGNGKAYGNGGRAGDDEGDGSGRGAEPYGGDGSGRADGEVRGDGSFHADGPAADDALARADAHVDADGRPHSVKTAAVELLNVLRPTVAVAHFVLFCALNLRWGAEWRNGEDGDDAYAEMFVQEVRRFYPFFPFLGARVRARKDFEWNGYEFPKGTLVLLDLYGTNHDPRLWEDDPEEFRPERFLRARFAAPDWEWGRAQERPGSEHIGPPGGDSPFDQVQMKFIPQGGGDPAYTGHRCPGEWITIELMKASLDDFLANRIEYDVVPDPEKQDLSISLETLSSGHPPQPLPNRMPALPRSGFVISNVRRKASAAAAVAAARAAQSVAATVVRSGCPFHRMEAPAGGVARRADRRPAVHPLRRRHAAPGREPPRAAGEAGGDDQHRADGNHYHVKELERAGLARRVGDKRAGNLADQLTIELVYQAVAATFVVEPRLAAWTDPRRAIADREIQAHVAAERTMKDLEGALADQVALESLVATGERLQRAAAQLLDRAAFDGEQQIASAAVEGRCGWPARRSAKTAPTRPSPTAPDTPPANKLLAPGAGMVDRIRDALSGRGKTD
>Consensus1_trim413/1-1142Percentage Identity Consensus MEQIPRDKGLDSTLALLREGYLFIPNRCRRYGSDVFETRLLGQKAICLRGEEAARLFYDEERFQRKGAAPKRVQKTLFGEGGVQTLDGEAHRHRKAMFMSLMTPERVARLAELVAEEWRAAVPRWEGREVVLFDEAKEVLTRAVCRWAGVPLKESEVKERARDLVAMVDGFGAVGPRHWRGRRARRRAEKWIAGLIEDVRAGKLQPPEGSALYVVAFHRDLDGELLDPRTAAVELLNVLRPTVAVSRFITFAALALHEHPEWREKLRSDAYAEMFVQEVRRFYPFFPFLGARVRKDFEWNGYEFPKGTLVLLDLYGTNHDPRLWEDPEEFRPERFLDWEGSPFDFIPQGGGDPATGHRCPGEWITIELMKASLDFLANRIEYDVPEQDLSISLSRMPALPRSGFVISNVRRKA
cluster was decresed to 4000seq by 40-100%, 90-100cov, 401-440aa for CLUSTALω
>Consensus2_/1-724 Percentage Identity Consensus MKQIPRDKGLDSTLALLREGYLFIPNRRRRYGSDVFETRLLGKPAICLRGPEAARLFYDEDRFQRKGALPKRVQKTLFGKGAVQTLDGEAHRHRKALFMSLMTPERVKRLAELTREEWRAAVERWEGADEVVLFDEAKEVLTRAVCRWAGVPLAESEVKRRARDLAAMVDGFGAVGPRHWRGRRARRRAERWLAGLIEDVRAGKLQAPEGSALYEVAFHRDLDGELLDPRTAAVELLNVLRPTVAVSRFVTFAALALHEHPEWRERLRSDAYAEAFVQEVRRFYPFFPFLGARVRKDFEWNGYEFPKGTLVLLDLYGTNHDPRLWEDPEEFRPERFLDWDGSPFDFIPQGGGDPATGHRCPGEWITIELMKASLDFLANLIEYDVPEQDLSISLSRMPALPRSGFVISNVRRKA

Cluster of CYP152_Consensus2_trim