Reference

Cytochrome P450 HomepageDavid R. Nelson, (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfold

CYP101-CYP299, CYP1001-CYP1050Cytochrome P450 HomepageComprehensive Bacterial P450sDavid R. Nelson, (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.https://drnelson.uthsc.edu/comprehensive-bacterial-p450s/
CYP1041, CYP1042, CYP1043, CYP1044, CYP1047, CYP1051-Fanele Cabangile Mnguni, David R. Nelson, and Khajamohiddin Syed et al.,More P450s Are Involved in Secondary Metabolite Biosynthesis in Streptomyces Compared to Bacillus, Cyanobacteria, and MycobacteriumInt. J. Mol. Sci. 2020, 21(13), 4814; https://doi.org/10.3390/ijms21134814
CYP1067, CYP1100, CYP1119-CYP1134Mohammad Parvez,  David R Nelson, Khajamohiddin Syed et al.,Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s Sci. Rep. 6, 33099; doi: 10.1038/srep33099 (2016).
CYP1094, CYP1136, CYP1146, CYP1156, CYP1158-1159, CYP1177, CYP1184, CYP1185CYP1137, CYP1320, CYP1136, CYP1719, CYP1762, CYP1851, CYP1912, CYP1976, CYP2029, CYP2287, CYP2337, CYP2725Makhosazana Jabulile Khumalo,  David R Nelson, Khajamohiddin Syed et al.,Comprehensive analyses of cytochrome P450 monooxygenases and secondary metabolite biosynthetic gene clusters in CyanobacteriaInt J Mol Sci . 2020, 21(2), 656. doi: 10.3390/ijms21020656.

D. R. Nelson's

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm,  recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output 

CYP1207

>CYP1207A10 (2766196179)Streptomyces rubrolavendulae---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTATTTENTA APAAAPTGPA TATATGPAPA TTATAAACPF TGAGAGTGAA 00500051 APGTPAGTAG AGAGPAGPEA APVAGAVRAG ERDGDQARDQ AGERAGERAG 01000101 RGAADRSAGF TERYLLSPAV NADPYPYLDA LRDHDPVHWS AMHRAWLVTG 01500151 HEQLTHCLRD PAVSADRVRP LMDAVPEGAR DDAERAFGIL SRWMVFNDPP 02000201 RHRRLRQVFQ DQFAARAINR YRGFVERATR AMAGRRAAPG RTGDLVADIA 02500251 RPLPALVFAR WLGVPQADAP SFWYWNARVG DLVLGAAQEE REYRTSLQSL 03000301 VNLEDYLAEL VRRRREEPKD DLISAVLAGG RVGTSVSEEE FVGMLTQMAF 03500351 AGGETTSNLI ANTLLALLSR PDQLAAVRED PARLVPAAVE ETMRFDGPSK 04000401 MSIRIAAADL ELDGRAVRAG DRLFLVTAAA NRDPDRFADP GRFDVRRGTG 04500451 GPLHLGFGFG AHFCVGAALA RLVAVSAVDV LVRERPGLSL VDEPLSWQPS 05000501 LLNRALTALP VRY

Alphafold2

Heme Docked

PDBj

CYP1215

>CYP1215A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1215A? (645410299)Streptomyces viridochromogenes DSM 40736---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MHTHASPASL LSALRPRTQE RLYDFYKELR SADDLFWDPR LDAWIGTSHA 00500051 VVSSAAGDPR FSSVRYPDIE AVSEELRPLA RVLSRQMLYS DAPDHSRLRA 01000101 LLSRAFTPRA VATLRDRIAG AVEQIITRAA PTGRLDIVAD LARPLPLTVI 01500151 CDLLDVPEQD RPALSSWSDP IAAAVGSSRL DADDSRAASQ SMARMLAYLR 02000201 ELLTRQDSPP APHTLRALLT TRAESADQDM DELLANCALL LIAGHETTTH 02500251 FIGNAALALL RHPQAADQLR RRTDLIPAAV EELLRYDSPV QLMLRRARHD 03000301 LDLAGRSIAA GQVVLLVCGA ANRDPAAFPD PDVLDFERPG GRHVAFGYGP 03500351 HFCLGAALAR LEGAVVLEAL LTRLPDLRLD GTAPQWQRSL NFRGLTRLNV 0400

Alphafold2

Heme Docked

PDBj

CYP1216

>CYP1216A1 (2563439385)Streptomyces sp. UNC401CLCol---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VNATPHVPPY APGRVPLIGH AVPLLRNRLA FLQSLRKHGP IVRISVGPKA 00500051 VTVINSPNLL QEMLTTQSKY FSKGLLFEKL KLFGKDALPV AEGSRHLTRR 01000101 RLMQPAFHHR QIDTYIRTMY TTVDPMIAAW QGMGPLDMKT EMQLITQNVV 01500151 MAALFSTTPN RDTGHAILKS VDTLFAAALR RALLPLPLLE RLPTPGNRRT 02000201 RRAGRVMRTA VSEVIADHLA HPDAYDDVVS LLLNTRDPDG TPLADEDILS 02500251 EVTGLLAAGS ETTAVTLTWL FHELARHPEL ERQLHSEVDT VLTSEPVTAQ 03000301 TLTQLTFTRR LVNESLRMYS PAWLVTRQAT EPVRLGGTDL RVGEDIVWSP 03500351 YTLHRDPALY PDPLRFDPDR WLPERPQPPK GAFIPFGAGK RQCMGNEFAL 04000401 AEATLITALI ASRWRLRPVP GPAVRPVGAI TLHPSTLHMS AQARTPVGHH 04500451 REAV

Alphafold2

Heme Docked

PDBj

CYP1222

>CYP1222A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1222C1 (2525024694)Streptomyces sp. Amel2xB2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRGHALTTHL TTDIDEIVTD VFQSTEGKRN PYPLYRRLQE LGQVHRSEQL 00500051 GWVATGYEVC SAALRDPRVI KGPEQIQPGR PDPAEHSAEA LLRGTMHRLD 01000101 PPDHTRLRRL VNGAFTPRSV AALEPDIQEL IDDLITPAVK KAEAGEPVDM 01500151 MSGFAFPLSV AVIGRMLGVP ASDWHRFHDV VLDLSSMVEL GFTGDELPKA 02000201 DAAADELIAY FRKLGAERMR HPADDLTSTL ANATEAGDRL TEQELVTMLI 02500251 LLFMAGFETT THSMGNGMFA LLENPEQTQW LRRNMDAMPA AVEELIRYDS 03000301 PVQFIAGYTK EPVELADGTA VPADEYLFLM IGAANRDPRV FSDPELLRLD 03500351 RGEAAPMSFG GGIHYCLGAG LARLEIRKIF TSLLTRFSAI ELAEPEPERR 04000401 SGLALRGYAR IPMWLTPA

Alphafold2

Heme Docked

PDBj

CYP1223

>CYP1223A1 (2563439385)Streptomyces sp. CNR698---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDQAAVRRS LRALFSPRGC PDPYPHYAVL REHAPVARLP DGTVVVSRHA 00500051 DCDRMLRDPV FRVEDDEWIA RTWPEGREHL TVFSLMGEMV NQNSPHHERL 01000101 RRLVSRAFTP RRVAGLRPAV TALVENLLTA LAERAGPDRP IDFMTHFALP 01500151 LPIAVIGELL GIPEADRPWF APRVQAVTSA IEQNLTGAAL DRADEATAEL 02000201 WDRLGKLTEL RQADPREDLI STLIAVRDAD GDRLTRRELL ANLVLLYSAG 02500251 YETTSNLLGN GLAALLDRPA LLERLRGEPD RVSVWVEEML RFDPPIQIAS 03000301 RWTGEDTELG GVRIPRGTQV VALLASANRD PERQPDPDVF LPDRPPTSSL 03500351 TFGAGAHYCL GAALARLEAQ VAFPLLLKRF PSISLAGDGA ARDRVTSLRG 04000401 HARLPLLMS

Alphafold2

Heme Docked

PDBj

CYP1228

>CYP1228A1 (2523209375)Streptomyces flavidovirens DSM 40150---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIISKPPFCP VRFQDSDLLD SGFHASTDMH ALWADLRENQ PLYRAEPGGG 00500051 REPFWVVTRH EDVSRVLRSH GEFSSRRGTI LCVLDLDTPD IASDNMMADT 01000101 DPPRHREFRE PLNKAFAPPV VATQEKLLRD LSRDLIQSAL DADVYDIAHK 01500151 TMMFPMAVTG TLMGLPPESW ERLVELVMMT IAYDDPDYST GNAQATVRQA 02000201 RHELFEYFQK EYAQRTREDV DPDVIGAMLA MDLSEGPMSQ EQVLLNAFVL 02500251 LIGANVTTPH ALCTLMTVMA EHPEQFQAVR ENPELRASCL QELLRWSSPV 03000301 TALMRYAVKD VELHGQTIRA GEPVSAWIGA ANRDERVFAD PYTFDVTRRP 03500351 NKHLAFGLGP HYCIGANLAK VGLEIFLDEI LDRVETVEIA GEVRHVASHF 04000401 VPGYKSMPVR FTPRKTEAR

Alphafold2

Heme Docked

PDBj

CYP1231

>CYP1231A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1231A5 (2516062663)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSLPAVRGGF EPFDVLDIEG YDFAFDDIPN LHELFAAARA RKPYAIVPFL 00500051 GTRAVLFLTH DLVTAAFKDE ESFPASAIYA ATTEPVLGHT IQCMRGAEHR 01000101 INRKLVVPAF RRKVVTEQVE AILEPLAHEL VDRFEARGEA DLVTEFTHGY 01500151 PVRVITRLLG LPMDDEERFR RWAVDLFHFP MAPEAAERAS REFTEYVTPI 02000201 VARRRIDPGD DLLSTLACTE AEGRRLTDEE ILSFLRLLFP AGADTTYLGL 02500251 GNALYALLTH PEQLARVSAD PDEELEWAVE ESLRWEPPVG LLPRVCPAPV 03000301 RDWQGLGLDI PAETPLIFAI TAANRDPAHY PDPDVFDIGR RVRPTLAFGD 03500351 GPHVCLGTWL ALAEIRAALK VLLQRLPAMR SVEPTRVRGL LGTALRGPES 04000401 LRVRF

Alphafold2

Heme Docked

PDBj

CYP1232

>CYP1232A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1232C1 (2585320586)Streptomyces mirabilis YR139---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSTLRTAAPG APVVELDLAE LRRDPYPAYT ELRRTAPLAW VPSVGRHLLT 00500051 RYEDIVHAEK LPEVFSSRED GSLLLRTVGP NMLREDDPEH RRLRAAAEPP 01000101 TRPRQVRDLW AGSFERTAHE LLDRIAGRGE ADLIADFAEP LVATNLALVL 01500151 GLRNASADDI ADWSRAMMAG NGNYADDPDI WLRAELATRA IEEAVAERAE 02000201 AVRREPDGSV ISSMVHAAEP VDLAEIQNNI KVIIGGGVNE PRDVFGVGAQ 02500251 ALLQRPEALE KVLRDPAAWK RVFEETARWV SPIGMYPRQL TEDHEIAGVT 03000301 LPAGSRVALV IASGNRDESV FERADEFDLD RPHRPHLAFG GGPHFCMGAW 03500351 VARHEVSNLA WPLVFSRLKG LRLVEGATAS DSGSAVGRMD GWVFRGLTSL 04000401 NVTWQAP

Alphafold2

Heme Docked

PDBj

CYP1236

>CYP1236A1 (2545383134)Streptomyces rimosus rimosus ATCC 10970---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSHPEALIPV PEVEPGTAGP PCAYARLRTE APVVKAQLPN GETGWLISRY 00500051 EDARAAFADP RLVRPLLSAW PPREGSDAPP PCLPTFLEMT GAHHERVRRT 01000101 VLPLFGRRRL AFMEPRVRAM AEELLDTMVA GADGGVADLV ASYAEPLPLR 01500151 VLCATVGLPY EDRETYLPHT LALLGASGLT MEEVLAALYA LQDYADDLIS 02000201 RKEKTDGEDE DYIRLLLAEA RRPDSEITRD DVVSFVVTML MAGYKTNIQH 02500251 TGNALLALLT HPEQLKALRE APERTGAAVE ELLRYVPLMN AINILVATED 03000301 FTLHGQNIRA GDAVVPVPAS ANRDPDAFAE PDRLDLTRTP NAHIAFGHGP 03500351 HACTGGHLTR MQLGIAIQVL LERLPGIELA VAADTIPWDE STPLRAPARL 04000401 PVRW

Alphafold2

Heme Docked

PDBj

CYP1237

>CYP1237A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1237A3 (2541455262)Streptomyces sp. HPH0547---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKGRSVSTQT PPRYPFAWTP PMQVPEALRH VHGSSAMEVT LPSGDVATLV 00500051 TRYKDVRALF ADKRLSRNIA RPDCARISKD NDLFMDPEID PDPPKHTQVR 01000101 SLVTKAFTAR RIEALRPYVQ QIADELLDEM AAGPRPVELN EALAFPLPIK 01500151 VICKLLGVPA EDRDRFRTYV DGFLSVTKLT PEEVGRCRQN LWKYLGDLID 02000201 SKRKEPGDDL VSELIRVRDE EDNRLSEHEL HFWCQGLLIA GYVTTASQIG 02500251 TGTAVLLHHP ELVREIQEDW SLVPSAVEEL LRTQIMGSSV GTMRYAVEDI 03000301 PLSDGTVIKK GTSVLLSEEG ANMDPEVFDK PFELDIRRQE NHHMTFGAGI 03500351 HYCVGAALAR MELQVATESL LRRFPGIRLA KPAQDLPRAL GGFMEGFTEI 04000401 PVDW

Alphafold2

Heme Docked

PDBj

CYP1238

>CYP1238A1 (2515596067)Streptomyces sp. HmicA12---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLCPLGPATR PATRYPRGTR TPPSLADPDV AREPYPLYRT LRDSHPLLHD 00500051 EPYGAWLLSR YADVRAALAD PRLVAPPPGR TFAHLEEGTH ATHRALVAPA 01000101 LQGRALAALK GGVERSAYVL ARRLTRRREA DLVEEFCRWL PTAAVVAALG 01500151 LPYEETVRFT AWSRGGLTHL GAAPADVEAA LRPLVAYRRA HPGDDLLSVL 02000201 CTARAGGRPV SDDLVTGLAA ALLGAGGEAT GMALAAFLAN LLDHPAQLAL 02500251 VRARPELTDA AWAESMRRDP AVHLVLRRAL APVPVSGGTI PAGATVACLL 03000301 GAAGRDASRF PDPDRYDLFR AAPGQLAFGA GRHRCLGVQL ARTTAELGSR 03500351 ALLEALPRMA WAPGFRPVPE GLSTRAPRAL VVRLH

Alphafold2

Heme Docked

PDBj

CYP1240

>CYP1240A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1240A4 (2616901772)Streptomyces atratus OK807---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VTLTPAEAPV SLHGAEHAAN PQRSYELLRR QGPVGVAEVA PGVVVNLVTD 00500051 YRAALDLLQD TDTWTKDTRS WLPQVPEDSP IRPLVEWRPS LFFADGEAHA 01000101 HLRRVITDSF GLLDPHDVRT LTFRFADTLL QEFADTGTVD LVRQYAQQLP 01500151 LMVFNALFGM PDEEGRRLVW ALGAMMDSDP ETQAAGGQSF GAYLETLYAT 02000201 KAAQRGHDLT SWFIDHPNGL SQEEAVNQIL ITLGAGNEPL GNLIANTLVR 02500251 MLSDDRYFGT LTTGSLPIQN AIDDALWHDA PLANYSVHFP KKDVTFHGAR 03000301 IPAGSPVMVS YAAANTCPHS GIPADGYRSG NKAHLAFAAG PHTCPARDVA 03500351 TLIATAAIER LLTYLPDIEL DVPAGRLTYR PGAVHRTLTT LPARFTPLTP 04000401 DAEGATPWSR SPSRPERDTP SASTSGRSEA 

Alphafold2

Heme Docked

PDBj

CYP1242

>CYP1242A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1242A2 (2516056179)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVTPAPTGP DLYSPELYLD GPPHALFAEL RRTDPVHWQD IPGQPGYWAV 00500051 LRHADVEYVS QNPAIFSAEA GGIILEDLEP ERLEALRGTL LSMDPPRHRA 01000101 YRKPLVKSFT AKVVGGMEDR IRTICREIFA RVGTGDVEFV HDVTSSLPTQ 01500151 VIGELMGLPP ADWDHIHALA ERNTAGEGGG AAIEMARYAI GFAASRRAEE 02000201 PREDITSLIL ESEFGGRMMT DLDFGQFFVQ LVTAGNDTTK TMLSAGLHAL 02500251 LTHPDQLAAL RADPALIPGA VEEILRWANP LHYFRRTATR DTELRGVSIK 03000301 AGDKVAMYYT SANRDEEVFT DPQRFDITRR PNPHLSFGFA EHFCLGVHIA 03500351 RLEGRVFFEE LLATHATIEA TGEPERLRSN LNNALTRLPV RLG

Alphafold2

Heme Docked

PDBj

CYP1248

>CYP1248A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1248A3 (2603775392)Streptomyces noursei---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAPFRYDPLS GSQLDNPYPM YQRMRDKCPI FFDETRNVWV VTRHADVLTV 00500051 ALDPETYSSE NAVRLSAGDE PDEVREVLRQ GWSLTPNLTE SDGEQHRQLR 01000101 AMVGRVFTPG KVAQLDPFIR KTIDELVDGF IDAGHTDIIE RYAWPLPLIT 01500151 MARILGIPQD DVPQLHQWSY DWLRLSESAH ALPDRVKYAH SVVAMQHYIM 02000201 DLFQDKEATD GDDLISLLLQ SAQEAAMDTV QVMQLVMNLI IAGHVTVTRA 02500251 IGNGIVTLLD HQLDVRESQL DEQRTKTMVD EILRFESPVQ GLFRTVMTDT 03000301 ELGGARLRRG DRVMVHWGAA NRDERVISDP EGFDIERRTS RHNLAFGRGI 03500351 HACLGAPLAR LQLQIAIPRL FERLPGLRLA SPDSWIRDRI LIARGFVSLE 04000401 LRWDQREKTT DGMALMTPRQ GLQTP

Alphafold2

Heme Docked

PDBj

CYP1251

>CYP1251A1 (2720606614)Streptomyces venezuelae---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VEQAAAVDPI LDLARPSILR NPYPSYDRMR ETGPVFWHEL LGSWVLTRHA 00500051 DCLAVLTDSN RFASDWRRAG EDIPAPLLSV QTLDPPEHTA IRHLLLDGFR 01000101 AQDRRALHDD LEGQIADLLA ELAGRPSFDL VGELAEPIAL RFVTAFLGVP 01500151 APALDWFVPM SRTVVDGMDA GLWPEKHEPA VAARAQLAEY AGGWLADPPK 02000201 DGLIAYVAEH AADSGVAETV LRNSLRAVLH AGYESASRLL GNAAAALLTT 02500251 PGALAAFRAT PATAVDELIR YDAPVQADAR VCVTDTELGG VTMKAGDPVT 03000301 LFLGAANHDP LRFDHPTELR LDRAPNPHLG FGRGAHACLG ASMAIRLTGS 03500351 VLGTLATDHP GARAVAEPEH RRNLTLRGLD RFEVCLRPDT GEEVRP

Alphafold2

Heme Docked

PDBj

CYP1253

>CYP1253A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1253B2 (2524979861)Kitasatospora sp. SolWspMP-SS2h---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VNGSAADPEG APGGASAEVR AALAAVFDPA LRADPYGPLA VLREAAPVLY 00500051 DEESGTRFLT GFADCQAVLA GPDFVVPDGP WLAREHPEWA ARPAADFFYA 01000101 SLLGTNGAAH ERLRGPLTAV FGPRRAAELS AGVEKVVDEL LDRFEDATSD 01500151 GGAADFQALV GYPLPVAVVG ELIGVPRAEQ ERFHRLGRDA ARLLEPVRSD 02000201 EDWRRADTAV TALREYFTGL LRERRARPAE DLASALLARA RPSAARAQQP 02500251 ADRRRPSAAR ERSSTENEPP LGERELADLL LLVFVAGFET TAGLLGTAVS 03000301 ALLAHPEQAA LLRADPGLLP GAVEEALRWD GPVLMTERLA ARPTEVGGVA 03500351 VPAGGSVTAV LGAANRDPRR YPDPDAFLVT RPDVRVLSFG AGPHYCFGAA 04000401 LARLETAVLL GRLSARFPRL APAGPPVRRA SACLRSFDAL PLAATG

Alphafold2

Heme Docked

PDBj

CYP1265

>CYP1265A1 (2519025958)Streptomyces sulphureus DSM 40104---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTRQAVVPAA DDTNTADAAF ASILFSPEGN QNPYPYYHTL RESSPVHQSI 00500051 LGMWVVTRYD DVLSTLRDKT IGKNVERFMQ GRFGGDWWSH AALRRMGTSL 01000101 LWANPPMHTR LRRLANQSFT AAVVAAQRER IVRLLDDLLD PIAEAGGGDI 01500151 VNDVAYPLPL SVVATLLGMP LEDAPLMREP MRNFQRTFEL GLTAQELAAA 02000201 DVGAQFSDDY FTEMIERKRR EPGDDLASSL IQVSDADGEA LSPIELVGVC 02500251 NMIIGAGFET TTHTLGNGLR ALLKNPDQMS LLRARPELLE GAVEEVLRYD 03000301 APVQMAPRML DEPRVIGGVE IPAPAQLMLA IGAANYDPTH FTEPDEFRIT 03500351 RDESPAISFG AGIHTCLGWR LAKLQAEVFY STLLRRFPQM ELAVEPVYRP 04000401 RATLRGLESL HISVTNS

Alphafold2

Heme Docked

PDBj

CYP1278

>CYP1278A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1278B1 (2603782164)Streptomyces noursei---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTRSGPATLG AAATHPDPYP FYAGLVAERP FGRDADSGLW VAAGAAAVSE 00500051 VLADPVLRVR PSAEPVPRGI VGTPAGDVFG ELVRMTDGAA QAQRRRSLIA 01000101 ALDAVTEERV VALAGEQAAR ATDWWDLQFG VPVRVVAALL GAGGDVPVEA 01500151 ARLIGDFVRC IPASATAEDQ AAAAVAAGRL LDLLGPQLRA GGGGLLDALV 02000201 RLSARDGWPQ PAPLLANVIG LLSQTYDATA GLIGNTLLAL ARHGRPDDPA 02500251 AFVAEVVRHD APIQNTRRFA AGDTTVAGHH VPAGATIVLL LAAANRDPLA 03000301 NPDPHAFRPG RADPQVFTFG RGAHRCPGRA LATAITVGVA STVTAAPVHE 03500351 GHHPSFNARI PVLKGQFP

Alphafold2

Heme Docked

PDBj

CYP1279

>CYP1279A1 (2518003056)Streptomyces sp. CNQ766---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDHLSWYDE IREAGRVHWD EETRSWYVTR FDDVWELLVD DRLGARSAKA 00500051 HIERMTPAQR ELCAPVLDSV SKWPVFTDAP RHTTLHRLIL PCFAPAETER 01000101 VTGVARSCLK SADPADWGSD DVVEAVIQPA CVAALAAFLG ISPADVTRVT 01500151 EWSSRLITFA GQTAYDHEVV TRASVALREF GDFVMRTCAA GNSVLSSSMR 02000201 RSLREGVLDR SEVVAVYGQL VTGFLQPTIS ALAMAIETLA DDGAMKAAFE 02500251 DAPDTFISES IRLASPFHFA PRRTLRDMQF AGRLIPAGQR VVLLLVAANR 03000301 DPRRFPDPLR FRMDRGTPPH VSFARGRYAC LGAGLTRHLM RAVLTALAEQ 03500351 PAGLAPPATV EWHVGRGMRM PLRLTRTKA

Alphafold2

Heme Docked

PDBj

CYP1282

>CYP1282A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1282B1 (2515822290)Streptomyces sp. LaPpAH-108---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VKELLVHYDP LAPETIHDPY PTYKAMRELG APCWNEIMKC WVVTSYDGCK 00500051 RVLSDYENFA ADWRRGGESM PENALSVHSL DPPDSTVIYR ELAATLRQVI 01000101 APDLSHRLRQ AISDRLRTLA GPPVDLVSDF TEPLACWFLS EVLEVPSLDT 01500151 PEIRSMGGAI HRAMDAGLVP EAFQPGRDAH RKLAALIEAW VASMTPQQPL 02000201 RTLVDRAAVE GLEGAMTLNS VRTLIANAFT SLPASLGTIV HGFARDPGLL 02500251 AGTVNEKRLD MAVHEFLRHE SPSQGTTRLC VADTVLSGAK IRRGEDVLVL 03000301 VSAGNRDPAK FDDPDQLRPE RWPNQHIAFG HGPHSCSAAM LSRVLLRELV 03500351 AVLADGSISI RLTGPVTYKP AATIRMRATL PAVVTPAS

Alphafold2

Heme Docked

PDBj

CYP1301

>CYP1301A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1301A2 (2538969138)Streptomyces mobaraensis NBRC 13819---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTPHTPTAA GGPSEEPLPR YPFSTKGDRL APELAELRGR CPVARVGTNS 00500051 GGEAWLVTGY DLTRHVLRDR AFARSVLGEA DSPAQDAPIL APELLDAMNH 01000101 LKKAGLRDEV LKALGRDQPE LPDAWVAEAT REGLDAMVRE GAPGDLQKHF 01500151 AEHVAARCML RLLGLPFEDR AYLAPRADAD LTMVTYSDEE LARNWEEIRR 02000201 HMDAHMAARR PGEPRGLADR LADLNARHQG LTGRQLSNIV AVLFVSGYED 02500251 FASFLGVAAF NLLQRPEALK TLRERPETTP QCVEELLRYS VVLGNAIPRY 03000301 VTRDTELGPA SLKQGDLVLL SLDAVNFDPA AFTAPETFDP SRSPNPHLRF 03500351 GYGRHHCPGA HLVRRHSDTA FRVLVDHLPD LRPAVPATEV PWHPHRMAIM 04000401 PAEILVTW

Alphafold2

Heme Docked

PDBj

CYP1313

>CYP1313A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1313A2 (2567284996)Streptomyces prunicolor NBRC 13075---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAMTDVRDGV AAGAPRAAGL PLLGSVLDFR RDILQAMQRG RTAHGDLVQY 00500051 RLGPVAVHGV SSPELAAEVL TDSDRFGKLG PDNPLRLVLG EGLLTSDDHK 01000101 SWLRNRRMMQ PIYTKQALAG MFATMGVSAA EQIAHMGDRY RTGAVVDLHT 01500151 EMMRVTLDIV SRCMFSTDVS RTLAGLGPDA VDVAINYAFD RLQNPLSPPT 02000201 SWPTPRNRRF HEVMRGLDDL IYRIIAERRR PDAPTGGGDL LDMLLAARDA 02500251 DTGQGMTDRE LRDEVITTFA AGHETTAITL TWAFYLLSRH REVLERVQRE 03000301 VDGVLGDDLP TPEDLAAMPY TLRVFEEALR LYPSAPILPR LVRRDTELGG 03500351 YRVPAGSRVL VNLYDIHRHP RHWTDPERFD PDRFLPETGK ARHRFAYLPF 04000401 GAGPHLCIGK HFALMEAHLL LCALVRRWEF RHVPSHRVVN HATITLRPRY 04500451 GMLMTMHQRT PSAARTAHVA GERTS

Alphafold2

Heme Docked

PDBj

CYP1316

>CYP1316A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1316C3 (2516060396)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTKTPPTPRP SLDLLDGDWY ANGPYPDYAW FREHEPVAWD AGNELWGIFR 00500051 HADVKDVEAR SEHFVNSDRN KGGYRPNLPA DPAIIGLDNP LHNKRRKLVS 01000101 KWFTPRGVRE WEEHVRETCV RILDAAVAKG TVEVVGDLAS VLPAKMIGRL 01500151 LGFPDEDWPK LRDWSERTIA LGGGPRYLSE DGRAAAGEFI RSALGLYETK 02000201 KACPADDVMS VWTTAHIDGE PVGPADVASD CLLLLDGGAE TTRTVIARTF 02500251 LYLLEHPDQW DLLRAGADLA VATEEFIRYS TPIHNMCRVA VGDQRVGDTV 03000301 IPDGHQVVLM YGSANRDPAV FAEPERFDVT RTPNDHITFG FGTHFCLGAP 03500351 LARLEIRVFF EELLARVREL RPVPGSAVVQ MPNAFVYGIR EAHVQLIG

Alphafold2

Heme Docked

PDBj

CYP1339

>CYP1339A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1339B1 (2712577900)Streptomyces avermitilis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPRRFPGPRG KWLVGNMAPY LADRIGWLRA TQKEYGDIVR LGPDTLVVHD 00500051 PELAHEILAR TNDTYLLETS QLAGRRQRAA AQARLDTWMA VRQGMWHGFA 01000101 GRLAEVHLAR LMAQVGLLLD LHAGDDKDLV ERCRATTGRL IVDFCIGGDP 01500151 RHAALCDEAV TRADLLFTSA QNALVQKETR RRFHRRPLAS AASTANAELL 02000201 GFLHDSVAER RSRGYSGSPR DLLDSLLHAS GPDDTNDLIV SVLRMAMFAS 02500251 HGVPGAALSW IVLRLASEPG ALQAVRKEAE TCPADGALSI ERLPLTTAFV 03000301 KETLRLHPPQ WLLTRTAIRR TTVGAYQVRA GQEIFVSPYV MHRDGRFWPR 03500351 PERFELERWT GDRIPYPRHT YLPFGAGPRI CPGSRLALAH LVILTMRLAA 04000401 DHVPQAPSIE DVNVTCDGLL LPANVRGGWT YRTS

Alphafold2

Heme Docked

PDBj

CYP1336

>CYP1336A1 (AFZ58759.1)Anabaena cylindrica---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLTEILQQGE KYDLLNPLFF ANPDATLHQM RTEDPIYWHS QLESWILTRY 00500051 VDIYNVIRDP RFSVDRGGKI GKSKSLAVKT KLDFCNQYFT QWMVFSDPPR 01000101 HTRLRNLVGK AFTPQLIESL HPLIQNFADE LIDAIQDSGK MEVIHDFAVP 01500151 LPALVTAKLL GVPQEDIPLL KRWSSNMFML FGAGWASEEV VETTYQSLVD 02000201 SIQYFDALIA EYRQSPSDNL INQLIAAEDH GSVLSDEELT ATCITFMAGA 02500251 YETTTYLISN GLLALLQNPE QLQMLQENPL LIDSTVEEIL RYCGPAFSVV 03000301 RRAIADIEIN GQLITNGQKI YCVLHAANHD PAKFPNPDKF EITRKENRHL 03500351 GLGQGIHVCL GAALTRLETK IAINTILQRL QNIRLDTEQL TWIPNLAMRG 04000401 LEELPIVFSL KGIPNKKIFN 

Alphafold2

Heme Docked

PDBj

CYP1341

>CYP1341A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1341E1 (2688565678)Streptomyces sp. CdTB01---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VAVTPTCPVH YDPLDEATLA DPYPVLAALR AHAPLVWHDT MECWLVTGYD 00500051 DCVRVLRDHE SFARDPRRLG RTVPEASLSV QVLDPPHQAA VRSAFMTSLR 01000101 AQDLSALQER ARRDFAARLE VLAPRPVWDV MCELAQPVAL NAIADLLGVS 01500151 PPPLSAWVKV SDTIMRSMDG GLNPDLVEPG RRAREELSEL VTSWFTATGS 02000201 PAGLLGHVRA RTTDAGPELA RYVVHTARVM FQGGYSTLAA AIGNCVDTLV 02500251 HNPPAQRVLR EAPQLRATGV DELVRYDGPV QGTTRLAADD VLLGGRTVHR 03000301 GEKVTVLFQA ANRDPACFEH PDRLVLDRSP NRHLGYGWGT HACLGTTVAQ 03500351 RVLVALVDAL ADQPGLLQAA GVARRRNTAT MRAFDQLPVT FQP

Alphafold2

Heme Docked

PDBj

CYP1369

>CYP1369A1 (2516059017)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MHPNEYDLTS EDAIAHPEPV FEEMRKRCPV SYVEAMDTYM LARYDDVKAA 00500051 MRDPLTFSSD RPFFGAGDSE LEEIQASGYP QVPTLTMTDP PIHTRYRKLV 01000101 NQAFSPKAVR ALEPSMRRIV HDLIDGFVER GSVEFVSEFA SLVPGYIIAD 01500151 ALGLARGEQD RFMGWADDFV DSIQGREILS RERMLEIQHH VIDFQKFFAN 02000201 EIEKREQAPG DDLISALLAA RVGGERPLDR PEILDLIRVF LTAGNETTAS 02500251 WISGTMRLLL SHRSQFEEVL ADRNLIPAML EESVRLVSPA RWTRRTIVGR 03000301 PVEMSGTVIP VGATVRPLWT SANRDEAYFP ESATFDIHRD TSAHMAFGHG 03500351 VHFCLGVNLA RAEARIAFEV LFERLRDIRS AIPLDDVRTL PFVGVNRLSA 04000401 LPLRFAAT

Alphafold2

Heme Docked

PDBj

CYP1373

>CYP1373A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1373A2 (2668577685)Streptomyces globisporus---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTQSAASPTS RVPRLPGRAL IGNTWEFKRD RLALFSRTFA ECGDLGVYTI 00500051 AGQDLYLANS VELVHEILIR NGGLFEKTDR FRSFARPLLG DGLLTATNEQ 01000101 HRPNRRMIQP RFHSAAVKTS ADIAARVTRT VADRWSDGAV VDVRREMVAV 01500151 TLGIVGQNLF SRDVLGEADE LGNALTDAIH GFDSQASALI PLTIEWPTPA 02000201 NLRYRRAIER LERTFYDLLA ERRAAVRRPD DWLNLLMEST YEDGTPIGDR 02500251 QIRDEALNMF MPGHETTATA LSWSFHLLAE HPEVYDRLLA EVDEVLGGRR 03000301 PTLEDLPRLP YALQVFKETM RLYPPVYMFT RQATVDVEVL GHVLPAGAAV 03500351 VFSPYALHRR ADYFHDPERF DPERFSPERE AGLPRHAYLP FGAGHRVCIG 04000401 NHHALLNGHI ALVTLAQQVR LSSVPGASPV MEPMVTLRPR GSLPMRVERR 04500451 SRPAAHDGPP TTWERPAHGE APAACPYADN S

Alphafold2

Heme Docked

PDBj

CYP1385

>CYP1385A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1385A2 (2685035268)Streptomyces griseochromogenes---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAVMRESPLA FITELARTYG GITRHRADGE TVYLLNTPEY ARHVLKDNGA 00500051 NYTKEGTPDD AMLRPLLGNG LLTSNGDDWA RQRQLTAPAF RPSSVRTFDG 01000101 IVTDATSGML ERWRPAIETG TPLAVDDHLT ALTLGILTRA ILGADLDGIG 01500151 EGFGRAVDAV NRCIGHYVPD PDPDPADTAR RFAGFSRARA FLDMVTRTLI 02000201 ASRRAAGPSA PGSGPGHAGN LLDTMMSAGH LVSDEDLRDQ VLTVVMAGHE 02500251 TTAKSLTWTL HLLDRHPHEA AKVREEVDRV LGGRPARAED LADLPVCQRA 03000301 VKEAMRLYPP VWLISRRAVG ADVIGGYHVE PGTLVCVSQW VLHRHPEYWD 03500351 APDAYRPDRF DGASLPSHLY LPFGGGDRIC VGQHFAMLEA VLVLATLTQS 04000401 VRLETVEGFE VEPEALVTLR PKHGMTMIAR PR

Alphafold2

Heme Docked

PDBj

CYP1386

>CYP1386A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1386A2 (2513528837)Amycolatopsis sp. 75iv2, ATCC 39116---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VTLRARQWIT LVRMEAFPCP ELGECGLLSD VSQSSDPATA DPVRDRARTF 00500051 DHLDPAQAAT IHETLAAFRK TCPVAHSEAH GGMYVVTRFD DLRHVARHGD 01000101 TFSSAAEVGA VVVAPETGGV IAPLFEQDLE EHAGWRRHLQ PFFTPPAAAG 01500151 HEDYIRRVAR EVVAGLAPRG QADLVPDLCA KIPPLVIASL LGLPEEQRPT 02000201 LAALVHGLAA ADSAAAAEAI GREYTEFLLA HIRSRRGKTG SDVLTSVVNS 02500251 EINGRLASDH ELLKFAFLLI AAGNLTTTDQ LASILLELAQ DPALRRRVAA 03000301 EPGLIPQLVE ESVRHESAVA ATGRTVVRET ELAGVPLRPG DRMLLTWGSG 03500351 NRDEEHFPDG DEFRLGRPRR PHLGWGAGAH RCLGLHVARV ELRVICEEFL 04000401 AAIPEFTLPE GFEPERTYGV IRGVKALPVT WPV

Alphafold2

Heme Docked

PDBj

CYP1392

>CYP1392A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1392A2 (2689319318)Streptomyces reticuli---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAGMDFLAAY DAIPEDDVPQ RVALLRTAMA TGRAALFAEL RARRPVFSTP 00500051 VGVFVTRHPD VLEVLSAPGT FTVGVFGPAL EDVLGAPFVL ARDGADVHWR 01000101 ERGYGQLMLA AEDAPRVREL TARIADEVLD EVTGRGADTF DLIQDYARVA 01500151 AARVAIAYLG FTGIEEETLH SCTRRMQWAF AINPGRDPEI HAAGVAAGRE 02000201 LHDRVAELIA RRRSAAAADG SDDILGRMLR TRLPAEYGFD DERINANLVG 02500251 YLTGYQQNAT QCTATALKEL AVRPEALAEA VRAAGEGDPA RFDAYVWEAL 03000301 RFHPFVPVTP RLCLRDHVLA AGTPRQTVIP AKSVVLACFA SAMFDEEVVD 03500351 APAEFRPGRP PAHNLVLGYG AHDCVGRYAA QVIVPELVRR VLLRPGVRPL 04000401 PGAERALDYA DTPFPQHYRV RSGRSATTPS 

Alphafold2

Heme Docked

PDBj

CYP1394

>CYP1394A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1394A2 (2574745599)Streptomyces sp. PCS3-D2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRATLAAEPL SLLSEEFARA PHPHYRRLRE QAPVWWDAET ECWYATGYRE 00500051 VNALLRDSRL SARIGAGFFG DLDVSARAGI QDVVDFFDAW PMFTDPPAHT 01000101 RIRSAAGPGY RPAGVRPLRE RIAREARELL ADCDPGTTDL LGRFVHPLAV 01500151 SVTCRLLGID DEHRESVLSW SSDIIEFIGV PQCEPDRAPR SRAAIRELQD 02000201 YLRTVILPMA RAGQGPPQIQ AFLDLEPRQG AALYAQLLTG GIEPVAASLG 02500251 SALVSLLDEA GDVLDGLHRG RIDAGQVAEE ALRYDAPFHF VPRSATEPIH 03000301 VAGATIRPGE RVALVVAAAN RDPAVFVDPD RFRIPVEGER DRPHLSFGAG 03500351 HHFCLGAGIA RVTLTEAIAA FAEWTAGRPV GRITADRSAA FCRTVWERVA 04000401 VGL

Alphafold2

Heme Docked

PDBj

CYP1408

>CYP1408A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1408B3 (2513529299)Amycolatopsis sp. 75iv2, ATCC 39116---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNRVELALAV APLPFLDAHA DDPAAMLDVR GGPAPKILVW HHETLASLFR 00500051 QDSRLRHPGS RSLGPLLGPK SMLWADGARH AAYRKLLGPP LRGRRLEAYH 01000101 SLITQATASA IDSLGTGAVF GLAAWTRRLA LDIVARIVLG DADPVLLRDV 01500151 TVWLDRALGS RHRTLAYRLL RGGLPVSGAE LDARLVAAAK SGREPRSLVS 02000201 PLLTGNGPLG PVPDDELRDQ VVSLLFAGHE TTASATAWTL YWLTTRPDLR 02500251 DAVLSELDAT TGDGSDPAEV PVLQAVIAEA LRLHPPVEVA GNRALPEAVD 03000301 LHGRTLPAGT VLTPAIYLAH HRSEYFPSPH AFDPGRFLGR RGVPEGYLPF 03500351 GGGTRFCLGS QLGQLEIRMI TAAVLRRRVL RCVNPRAGVA KLRGHAMAPS 04000401 ARLRLEVVAC RD

Alphafold2

Heme Docked

PDBj

CYP1416

>CYP1416A1 (2584334380)Streptomyces leeuwenhoekii---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSSPHVRPS QGAAPLPASG CPVHSEPVRL NSEAVQGSLA DLYEPWRSQY 00500051 GPVVPVELDG GVPAFLVIGH RTLREVCSQE SLYSPDSRNW ADWRAGRVPN 01000101 DWPLLPQVAY QEGSTRFLSG PEHRRLRGVL ASGLAQVEAA SARRYTEWVA 01500151 DRLISRFAPK GRTEVVADYA APLPLLVMLR LLGLPHEAGE QLLPAIFRLL 02000201 EGGPGAHRAN EEISDIIGRL VVARRAKPDR DLISWLIHGP VDGGPALSDL 02500251 EVRNLAWLTV MAGAGGTTGW IGNAMERLVC DEKVHTLFLA GKVTIAEIMN 03000301 ETHWSNPAVQ NVMGRYPLHE VRLGNYHVPA GALLVLGLAA ANADPEVHVD 03500351 DSGHTFTNES HLAFGSGPHE CPTAAQRLAK IIGQTAVERF LARCRTPRLR 04000401 DDGAVQHGGS VIVRQLTKLA VIFTADTRTA VRHTASGTDA LGESSRSSHY 04500451 LFPPRMLTQL TSHITA

Alphafold2

Heme Docked

PDBj

CYP1417

>CYP1417A1 (2656593308)Streptomyces ambofaciens---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VTTPPFTPTP TAPGSLPLIG HAHRLARTPL PFMASLREHG SVVRIRIGTA 00500051 PAYVVTDPAL TRKVLVTDAA DFTKGGKIID ALRVFFGDGL ATVADGDVHL 01000101 RNRRLMQPMF NKAHIATRGD AMINQVQSMV DAWEDGEHRD VFADMNNLTL 01500151 AAFLVALFGT DLPPHLQEEF TALMPAIMKG TIRQTILPPW ANRLPLPANR 02000201 AHAARVARLR TLIDQAIDHH TSRLSTTSAT TTQATGCPHA DRPTGLFETL 02500251 LTAPEPLSRQ QLQDEAITLL TGAIETTGTT LAWSLYEISQ HPHVEQRLRA 03000301 ELTSVCQDRP LRYTDVDQLH YARSVLQEAV RKYGPAWMVT RTATRDTKLG 03500351 GHAIPAGADV VWSPYLHQHD PEVFPDPERF DPDRWTRGEA AATRGSFLAF 04000401 GDGRRKCIGE NFAWAELQII LATILQNCSH LQLTTRPPRP QAVVTVKPDR 04500451 LTMSYSRPTT TRNEDDEGQA HCPTAQPSTP 

Alphafold2

Heme Docked

PDBj

CYP1418 (NoCys)

>CYP1418A1 (2584331691)Streptomyces leeuwenhoekii---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VNLSSTGTVT YPFDDGKEKA LAPYDPELRA SSGLAEVRMS FGDPAWLVTR 00500051 NVEVRTALMD QRLRSREPSM DPGAPRMHPD TPSIPVNIAA LSGPEHVCGR 01000101 KVMAKSFTAR RVPEFQPRTT ESSRGLVDRL VGNGPVADFV DVFALSLPLH 01500151 VICDMLGVPA GDVGQIMEWS DAFLEMKFTE PAPQQVGEYV TAFTEYLGGI 02000201 IAARLVTLGE GVMGEMAEAV AAGAATLPEA AMLALGLMVT GLENSSIALA 02500251 NFVYVLDRTD QRRALCDNQH LVPTTVEELL RFLPLSAGAV LAGQATEDVA 03000301 VDDHLVRAGE TVILAYGAAN RDEAVFHQPA EAELDREANT LTELTTRMPE 03500351 LRVHAADGEL AWLITRQSRG FAELVPRQAT FARSRRPARP LATPAGSPST 04000401 SSTRTSGRHT ESTHRTPLLD GQTLPAAALA VSW

> 44% Blast WP_253777002.1 cytochrome P450 [Goodfellowiella coeruleoviolacea]---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VMSRDEINDP LLYPFADGQQ LRLSPRYAEL RQTPGLPRVR LPHGAPAWLV 00500051 TRYEDARFVL TDRRFRARVP GQTPDTPRMT PDPPPPEGDI VQHTGAAHMR 01000101 LRKVLAKAFT VRRVEALRSR TTGIVVELID DMEQLGPPAD LVAHFALPLP 01500151 MSVLSELIGI PPEHHDQVMT WSGALLGREL TTVPPHQTAE HVAAFTDYLT 02000201 NQIRHRRATA SGGVISELAL ACTRGTLNAR EAAVLALSLV RSGREATTVA 02500251 LTNFVHLLSS TRQWKRLVDD PALVSTAVEE LLRFTPLSAG ALIPRCAVAD 03000301 AEVGGHLVRA GEFVIVPFTA ANWDETAFTD PGDLDLSRRR NSHIAFGFGP 03500351 HRCIAAELSR MVLQVALTQL VERMPELCVA VRTDELPWLT TGQFRGFARL 04000401 PVSW

Alphafold2

Heme Docked

PDBj

CYP1419

>CYP1419A1 (2641091833)Streptomyces pristinaespiralis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTAPLRAAPG PARARRRDRR VYLRSHPLLF ALLAATRGRA VRRIGRTVLV 00500051 HEADAYRRVL TGLPLDRTAA GTTGGAARAA LNAAGHHDAG VLFDQEGDGH 01000101 RAGRRGLAQG LGAAGVEDLR AVWRPLLAGR LEPLAKGGEV DLVALARELS 01500151 GTVVCALLGS GASPAAVAAA ASELAAASVR GELPGPRRPG AHAASARATR 02000201 RLRQLLGEGD DALSAMVAVA AVNTTVAALP RAVAWCADAG LWDQAADERL 02500251 LLPLVDELLR VTAPSPLLPR VAAADGVVAG CPVRSGDRVV LVARHAVDAH 03000301 TKDPDARRPA APDVARLVFG AGPHTCPGAR LARLQLADVL SALSVHRPVV 03500351 TRAVVDRGAA LPSWRKLVVR SSEVVR

Alphafold2

Heme Docked

PDBj

CYP1420 (No Cys)

>CYP1420A1 (2541755917)Streptomyces albus J1074 (Partial?)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLPTEQRLRR RTQLSHGLVW LAAAQGDPYA ALLRGLDTDP RPYWRLLSEE 00500051 RLSRSATGAW VTARHAVARQ VAAEPALRAL PPVEGVDAPA SGTGASEAAH 01000101 HVEEGWERAL ASAAREPDLV TLARDGALGT LSRAWGLDSA GERTLRVAVE 01500151 LTGAALDDPF YPQSLAATRG IAEGVAALRS LPEMGRRGEE LLAAAGVPMV 02000201 TSLVVNTLTP HPSADRRPDL PGLWDQLRAE PGRAASAVRE TLRWAGPVQL 02500251 HATVADAACD LAGRRIEEGE QVVVALGAAN RDPGVFTHPD RYDPDRPADE 03000301 LSAVLPPGLE WSPVVAFAVA VAEEGLRRLT AGGGGAAPAG PVVRRAAAPV 03500351 SWAPAVRLSE TI
>CYP1420A1(2603775977)Streptomyces noursei (Partial?)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTVEHSRLG SALQVARGRR WAFGANGDPY AMLLCGHDDD PWHWYEHIRA 00500051 SGPWFRSRTG TWVTTSHTQA VEVLDDPAFT RAWSSAPDWA APFAGIQAAA 01000101 PPDPLPDRET LVRQYAGLMP TSAARFDLMR DFAWEGALRG LAAQLGLSSA 01500151 DTGRLDAHAS ATRVSPDAVL SPQQLLVTER AIAALADAPD GAAVLGVELA 02000201 ANTVCRAILA TCRTEGLADR LAADPELATR IATETFRAEP PVHLERRTAL 02500251 EDRELACARI TAGSEVVVAV AAANRDPQVF ADPGRFDPDR PEAPAVLSAD 03000301 RGCRDGLAGF VVAHVEAALR AAAQTLPRLS VAGPVIHRRR SPVLRGISRC 03500351 PVEI

Alphafold2

Heme Docked

PDBj

CYP1422

>CYP1422A1 (2633777675)Streptomyces xiamenensis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VATSLTLTPV GEQWPERTPG PEHIYPLHGP DFARDPFSYY DNLRRHVGAV 00500051 VPVSLEETHR IRGYLVIDHA AQLEILRDQQ QIWTRDPRWY RDLAEGVLPA 01000101 DHPLIAQLAY RRSRLCSEGA EHDTLSGPGN KALARMDTLR VGDLVEELAD 01500151 QLIDSFFQDG DPEAETVEVD ILRQFALPLP LLVLTRLAGM DEREALASGT 02000201 ALHAMLSGSA GPQHTREELA SLMAGLVEHK RKEPGPDLVS WMLHHNQDDA 02500251 LTPAELSEDV WLQIAVGRGA STAWICNTLL ELMTNERLNE DVAAARCSMD 03000301 QAMNHVMWTD APIQNLIGRW ATRPTWLGGF RINAGDMAIV SLGAAAADPA 03500351 MRSVGLDTSR TNNAHLAWGS GTHGCPAPAR ELGALIVRTG LERLWDRLPD 04000401 MELAVPREAL EWSQPHVART PLALPVRFPR PSADLPNGAS EEWTLTSGPS 04500451 ETAPAARPEQ QHSPFTTPPP RTASPTGKDG SAAQRGKLPL WRSPAAWWPK 05000501 R

Alphafold2

Heme Docked

PDBj

CYP1423

>CYP1423A1 (2629136126)Streptomyces vietnamensis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSATAPVPP PSLADGVRAP EWDEGLDCWV VADPALALQV LNHPGFASGT 00500051 FERSFQLYMT DAARAEYREL TEFLRLWFVQ ADAPEHTALR RPVQRMFSAS 01000101 YVRSLVPRIE EITDECLTAL AAGAAPQDVM PTVAEGISGK VMAHVVGIDV 01500151 PPPVLHEWSR TLSLFIGAMY RRDYAQAAHR VMVEMSEALV GARALDGFPT 02000201 DTPEDRARTT ATWAMCLFGG LETTASLLGS TVLTALGDRA LWDTVVRDEP 02500251 GAVTGLVESV LVSRPPLRHL GRVAAYDQEF AGARLRQGDL VLVSLTGQGP 03000301 LDEVVPEAEA PAACPVTGAA APAGGDRNLV FGHGPHYCVG APLARSEAAV 03500351 LLRRLAARFP HARLSDEAAV WGPNLSYVGL DHLYVDLGGE A

Alphafold2

Heme Docked

PDBj

CYP1424

>CYP1424A1 (SLINC_1335)Streptomyces lincolnensis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSAVASFNP LDLTHTDDPY PRLAALRRDR PVSSPMPGWH VVVRDEDVRA 00500051 VLADPEVWSS RRNNTSVQGD ERELALSQVD PPAHTRLRRL LSPAFARTVL 01000101 AELEPVVRER AETLADRVGN GSDLVPAFTA PLPRAVLAAF CQVADADAPD 01500151 YDRWAAAFTD LLGHRAHSDW PAFERWALRA ADGPALGGVL ARLDRTEAVT 02000201 FLHFLIVAGV PNLRHALGNL VLRLVAEDHW RSDGLPEAIE ESLRLDPPAL 02500251 WQMRTATRDT AVAGTAVRAG QRVIAVVASA NRDPERWGED ADTFRPDRPG 03000301 LRAHLAFGKG PHACLGASFT RLQLRVAAEV LIERHPRLRL APGFRFRRAG 03500351 DFMSRGPAAL PVLCGVSSP

Alphafold2

Heme Docked

PDBj

CYP1432

>CYP1432A1 (2513527572)Amycolatopsis sp. 75iv2, ATCC 39116---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSNITSSMRP SDLDLYHPEA LLDPYAHYAK LRELGPVLFE PEREIWLVPG 00500051 YTEASGVLRN HKEFVSGDGV TYEGVGDRLE RYPLIENDPP VHTRIRRAVQ 01000101 PAFTKSPIEQ LRPAIEAAAK SIAEKAVALG EIDAVQAVAQ PMPDKAMELL 01500151 TGLTPPSAQT LADWSDAVVR AQEPAASPRH SELLIEALNW LTEVGVPGLP 02000201 AHCFGRLITE SGGANGGLEA DGSERLMTLA SIWLAGIDST GAMLGNAIDA 02500251 FIEHPDQWEL LRARPDLIPN AVEELLRFEA PFRVFYRRTR AETEIGGTTI 03000301 PAGAAVCAVL ASANRDPRQF PDPDRLDVTR ENARTHLSFG TSIHLCLGSP 03500351 LARLEATSML TELTRRVLRF ERTGEPVRSP SQTMRKFDSL PVRLVPAPGV 04000401 A

Alphafold2

Heme Docked

PDBj

CYP1441

>CYP1441A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1441A2 (2513527572)Amycolatopsis sp. 75iv2, ATCC 39116---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MMSTTTVPAR AAVPRAALPD ALVVGALVLA PNLAQGLFAR RPAVVRAAAA 00500051 AGVDGAAVRQ LSAMASRSTR PVRVGGTVLL LDPRDADRVL EGPVDVYAGD 01000101 PDPKRLGMAH FQPGAVTISR GEAWRERRRF TDAVLASAPA LAPRVAECVA 01500151 EEIARLPART GWRPWNRAFQ RIARRIVLGD EAAGDDALSV TLERMMAEAN 02000201 RLPRRPSRRL PAFRASVEGH LERAEPGSLA AAIADTPAGP DTEPCAQVTH 02500251 WLFALGDTLA ANALRALAVL GQRPADAERA LAEADHAAAC LQETMRLWPT 03000301 TPMLSRETRA EVVWDDGTVL PAGTRILVVN TFFHRAPAHV PDADAFVPER 03500351 WLEGTPAGVN HFSRGPQRCA GADLAVLTGR EALRAALMRT LTIRTPRLPA 04000401 EPPMPYALDH FRIEAVLA

Alphafold2

Heme Docked

PDBj

CYP1448

>CYP1448A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1448A3 (2524980294)Kitasatospora sp. SolWspMP-SS2h---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDSLRHELTR FIQQPAAQQD PPALYRRLLT EAPVLDLGPL YVVSGYQEIL 00500051 TVLMNPGTNV DPTTVGLPRA GNTALLKVVN RMLPMRDGAD HTRLKRLATA 01000101 AFSHRRLEQM RERIESTAAR LLAPLIAAGS FELVADLAVP LPVAVSCAIL 01500151 DVPDSEQGRI TGWASLVARS MLDPFGSGPE AAELDAEFAE FHAYVEELCA 02000201 ARAADPGDDL ISRLAAARGE GRLDTEELLA FVVMLLANGL ETLTSGLAVA 02500251 AWELIRTPGL TARVREEPAL AAAVFDEAQR LGSPVRASAR ALTREVALGD 03000301 TVVPAGSVAM LLYAAANRDP RRFADPDRFD LDRAERQHLA FGHGPHHCLG 03500351 APLSLIAGSA VLRGLAEADA TRELSTTLTD ATARPTEQFA FGGVRELPVV 04000401 CPPRTAPVPE LMEVAA

Alphafold2

Heme Docked

PDBj

CYP1453

>CYP1453A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1453B2 (2688562468)Streptomyces sp. CdTB01---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTPQHPEDF LHSIRRRSAR EDGIFWVDDQ RLGVFEPEAA RRVSATNWHR 00500051 YVMHDRLVDL VRRRRSPEIL WSRVRSTWMT RLHALATAEH QGRLVERMER 01000101 VMDERLGEDV DLTMLMQDVT LRSLLPLALD GLTCAEEELV RRDLEMKLSR 01500151 LVSPEPGGPL RHLRHIVVQI RAGLVVRRVL RQRADGRRDR EPDLADPFVD 02000201 LLPELGMDRA LDVVTTVLTA IGGPPGTAAA SLLYEFVRHP EWQRRLADEL 02500251 CAVDPSEFRA APTRAAPVTH RFVKEVLRLW SPPLLLVRRS TYAFDLGRAR 03000301 LEPGQWYLLS PHLVHRDERV WKQPEVFDPD RFLPGAPHGP ADRTSYVPFG 03500351 WAPKRCVGAN VGTFQLMALC HLLCTRYRLT ARRPDEITMA LRFAPVPEDF 04000401 RGRLTLR

Alphafold2

Heme Docked

PDBj

CYP1455

>CYP1455A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1455A3 (2547412394)Streptomyces sp. TOR3209---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MHREAFIDWA DRDPRCPTHH LPHDLGASDD PYPVLEPLRS AGPVVRVRLY 00500051 DRFDVWWVTT HAAASAALEA TELCSDQRRV HPSLAQHVSP MRLGLIDKDG 01000101 PEHTRLRTAA LSASSGGALP AFRQHAGALC AKLVGALPPQ RTADLVPTLI 01500151 GPLVDETIGR FLGLPQQVDA VLRAHADTLV RPMTDEAIRT EITEARRALR 02000201 RHVRALPQLR PPCAGAAGSR GEARPDEWTD VLLQMYVAGR RSTADFLATL 02500251 VLGVLAQDRW AEGCRTPLAR RRLCDEYLRF DGPIVRGVWR FARTDTVLAK 03000301 TSIRAGDIVI VSLALANRDP TAFSRPTGLD PGRTPNRHLQ FGHGAHRCTG 03500351 VPLVYGLADA LLTTLSRQYP DASTTGDDPA CRSGRNSIFR GPARVLAALG 04000401 PRQPV

Alphafold2

Heme Docked

PDBj

CYP1459

>CYP1459A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1459A3 (2654648797)Streptomyces sp. CNQ-509---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVAERGFRRD PIGFVERAAR AHGAPFRLPG GALCVADPET AVQVLRDNGR 00500051 RYEEVTEFLR PRGGRLADPV TQVAVGRAAR RVLRARLAGR ENLFAQVVAG 01000101 LGEDSRWPHA GHVMVHDALA DVLLHPDSSP RLRHLMARCV RRHDVLIRSS 01500151 NPLARVPQHL LRARLNRAVA EEVRIRRRAD SATGALPAAP KDLLEAVRTA 02000201 TPADTDALEI AGAYVQLFRT SVAPVAHVVA WALLLAAHAG VDLAAVRPDR 02500251 AVREALRLWP VAWLLTRTVR LPHEVAGAAV APGETVAVCV YLLHRNEDVW 03000301 PEASRFRPDR WAHATARGAY LPFGAGPRAC TGGSIALDVA TALVAALSHG 03500351 SRLVVHGGSA RPRVQGIITP PDFRLDRRAT TASSAQAVVS SDDGR

Alphafold2

Heme Docked

PDBj

CYP1460

>CYP1460A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1460B1 (2654648797)Streptomyces sp. CNQ-509---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSAVGVGAGQ RLPAPPRVPL VGGWQARGLR MLQDPTEYFL RQYRRFGGIS 00500051 AWDATRPKHV FVFSPEYLRL LFAEPEVFIV DAFREVRMPA DSSFLRLSNG 01000101 LLRLNGDTHR SHRRVVQHGL HARRVVRYRD TMAELTEELL AGWRTGETRR 01500151 IDQDMGRLVT RTAMRTILGV DAPAEADRLQ RLIDRLLAVA ASPATLLFPY 02000201 DLPGTTFRTA LRVSERIEES LRALIAERRT AAGSDVLTAL IAARDENGTA 02500251 LTDEELVAEA YTSFCHDSNI STLTWTLFLL DRHPDVLDRL TAELRDTLGG 03000301 AAPTADDLDR LDYLDAVVKE VLRLLPPAPM LLRYTAREAD LGPYRLPSGA 03500351 LLFYSPYVTH RLPGLYDDPL RFDPDRWRDL KPAVYEYLPF GAGAHHCLGK 04000401 HFALLEIKTV LALLLQRFRP ALAPGTRVDR AMRISFVPKH GMPMVLHPVG 04500451 ARPEPVPVRG NITESVRLA

Alphafold2

Heme Docked

PDBj

CYP1469

>CYP1469A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1469A2 (2652742113)Streptomyces lydicus A02---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSRVNDFSPP LRYPFALGRH GTPPPVIAWA RKHHPVCPVI LPSGRQVWML 00500051 TRKDDITRLL TDPVFSRDLV YPGAPRFVGE DFTAVPGGIF NLDGADHARI 01000101 RHVLAPHFTP PAAARHAEMI ARHAEGLLDA METGPNPVDL VEAYAAPLPL 01500151 RVSCEVLGIP LQQRAAYLDA FRTQTSLVVT AADVAAATAA TLDLTEQIIK 02000201 GKRADGDHSG PLGELVRAEA EGVISDEELH GTASYLLVTG SEPLVPPLAT 02500251 GAVTLMRHPD QLNACVAEEE LWPRAVDEVL RYHHNGVLGL PRVATEDITL 03000301 HRVRIAAGDG VCAPMLGATW DPRHYRCPEK FDIHRTGDMT ATFGAGPHFC 03500351 LGGAFARVFL TTAYAALFRR FPDLFLAVPE DDLPWQTDIQ LIRPARVPVC 04000401 W

Alphafold2

Heme Docked

PDBj