CYPSD - Bacreria251-299

Reference

Cytochrome P450 HomepageNelson, DR (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfold

Cytochrome P450 HomepageComprehensive Bacterial P450shttps://drnelson.uthsc.edu/comprehensive-bacterial-p450s/

D. R. Nelson's

Bacteria_1

Bacteria_2

Bacteria_3

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm, recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output

runner_P450.py

align_P450.py

CYP251

>CYP251A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MQPAASRSVP VAPGAVPLLG HALKLWRDPL GFLKSLRENG DLVRVNLGTM 00500051 PMYVATSAEL IHEVTVRQAR SFEKGRFFDR LRPLAGDGLA TANGEVHRKH 01000101 RRLIQPMFHP GRITMYAETM SSNALALADS WTAGQEVEME QAMAQFAIET 01500151 LATTLFSTDI GQPAVVAIRE NLPVVLQNLL IRAASPKFLD RFPIRPNRDF 02000201 DAAAAKLRSV IDEVVATTRR AGTSEHNDLL SVLLGARDSE TGEYLSDTEV 02500251 RDELSTILFA GAETTASTLS WTFYQLAEHP EVEAELVAEI KDVVGDRPVS 03000301 IEDVPKLEAV RRVLDEVIRL HGVTMLMRRT TAPVELGGHL LPVGTEVGFS 03500351 LYAMHRDPTL YDDPDRFDPD RWLPERRGDI TRQAYIPFGA GNRKCIGDAF 04000401 VWTEATIALA TVLARWQLRP LPGHTPKEVA SAVAHPDRMP MTVLPRDA

Alphafold2

251A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP252

>CYP252A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSPSTRSETA DPQPPGERGT MSPTLSGHLR RPPGPAGYPV VGCGPDLVRD 00500051 LVGTFTRAWR EYGDIVSFRL PGKRNLFLIV HPDHIKHVLD DRQPNYPKDP 01000101 LSVGKFEPWV GQGLFTSNGD FHFRQRRLAQ PAFKGSRISG FGPAMVEATS 01500151 DLVRDWHGRA TREETFDITP EMFRLALTIV MQTLFSTDVS NRAGELARAV 02000201 RICNAYTNVR LQRFVELPQA LPSKARSRFL RARATLDSFV YGLIAERRRD 02500251 PAPPDDLLTR FLNAQDEQTG ERMSDKQLRD EVVTMFLGGY ETTALSLVWA 03000301 FSLLSRHPEA ERKVRSEVAE VCGDREPAAS DLPRLRYLRA FYQEVLRLYP 03500351 SVWTLSRSPL EDDEIGGYHI PAGSQVFISP YLMHRHPGFW ENPEGFRPER 04000401 FEKSATGGAP LYAYIPFSRG PRLCPGSSVA VLEAPLVIAR MLQDYRLTLA 04500451 GGHVCEPTSN VFLYPRAGMP MRLQHFKSTG EGAA

Alphafold2

252A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP253

>CYP253A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MCPPSSPTTY PSSPCDPPPL RKGLVRMTTT LVPPGPRGHP VLGSIREIQR 00500051 DNVSAFMEAF RHHGDIVNFR GPLRINLLAH PDYVQHVLRD QHKRYPRPRK 01000101 VQGCLSTIVG DGLVAAEGTS WLRSRRLTQP AFHRDILRRF GDSFTASTAE 01500151 LLDGWERRAR DGEPLDIKSE MMHLSLANLA RALFRTEWTD EVSRIEPAVQ 02000201 EALGFTHRRM TSPVDPLKFP SAARTRFRGA LETINSLLYP MVAERRRDGG 02500251 GDDLVSLLID AVDPESGGMF TDEQIRDEVS GFFVAGHETV STALSWTWYL 03000301 LSLNPESRRR LQAEVDEVLA GRVPTVDDLP KLTYTTMVLQ ESMRLYPPIF 03500351 VYMRCAAEDD VIGGYHIPEG RWVVVCPYVT HRHPEFWDNP EGFEPERFTP 04000401 ENSANRHRMA YLPFGAGPRK CIGDSFAMLQ MPLVVAMIAQ RFRLDLVEGQ 04500451 QVFPEPAISL RPRDPLMMWL RPNEETP

Alphafold2

253A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP254

>CYP254A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VTTTQLPDRL IVDFDVYDQT LAMPEDVFQE RAAALRAIGP VVYSKAHGGH 00500051 WIVTRYEEIH QVLRDPETFS SYPNNLVNAG QGKFIPIELD PPEHTYYRQA 01000101 LQPLFSPKRM KELEPRIRDV INELIDDFAA RGEAEFISEF AHELPTRVFL 01500151 ALMGWPLEDA EMFTTTTDVA LQGVPGGTEE ESATAREAAA NQIFGYFGAI 02000201 VAGVRSGEIT SDTLTAQIIN APIEMGDGVR LLTDEELYRM FFLLLIAGLH 02500251 TVQGSLAWAI IHLANNPGQR QEIVDDPDTV SAAVEEILRI EAAVIAGRRA 03000301 TRDVEIGGVT IREGDQLIVL LCSANRDGAE FEDPDELRVD RSPNRHLSFG 03500351 AGPHRCIGSH LARIELKLAM EEIHKRLPDY RLVPEDPPIL HATQVRGCIR 04000401 LPITFTPAS

Alphafold2

254A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP255

>CYP255A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTSTTWIES ITMEELDRDP NPIYDRLRRE APVAFVPAVG MHVVASRDLC 00500051 LQIAQDSETW STVIAPSGGR TFGKGTVLAA NGEQHEKIRE WIDPQLRPSA 01000101 VDSYVEALVR PQARSLLEGI EDLGAADIQE AYFAPISVRS VGDLMGLTEI 01500151 PSETLVRWFE TLAQSYGNAE VDENGNFANP GPFEAGDRVK AEIVAAVGPM 02000201 LDHWTEHPDH TLISHWLHDG MPDGQVRDRS EIYPNIYVFL LGALQEPGHV 02500251 MTTTLAGLFQ HPDQLERVID DPTLIPRAVN EGARWVAPIW SAAVKVAGRD 03000301 VTIGGIDLPT GTPVMLAYGS ANRDESVWEN AEAYEIDRPI MPHLAFGAGN 03500351 HACAGTYLGT AIVRIALEAL FETIPNIEPD PDRAPQFWGW TFRGPQGLHV 04000401 TWEV

Alphafold2

255A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP256 !!!FL!!!

>CYP256A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTVDEPVLD TTYPFGPVEP TEAAARYAAI AAERPMTKMT MPIGGDVWVI 00500051 HRESTARAML ADNRFVREPF RTGERPVPYF VEFPDFLKST IQFEDPPHHT 01000101 KLRKLVQRSI SPKRVRSMRD SAVEFANSLL DAMIEKGAPS NLVEDYAVAL 01500151 PIQMISNLLG VPPEDRPKFQ KWSSATLSVA DMPQDEVMAN MGELVQYMME 02000201 LIDARRREPR EDLLSDLANA RDRDESLTDE EILPIALILI VAGFDNTANF 02500251 ICAGVLSLLH NPDQLEILLE DVDGVAPTAV EEVLRHGRFE LGRPVAGGGG 03000301 LVPFVATEDI ELDGQMVAKN EAILVDPGST GHDGAAVEHG EKFDVRRANN 03500351 PHLTLSYGLH HCLGAPLARM EMQVAISELF KRLPGLALAG DVQIDDTNLT 04000401 QPITMLPVTW

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP257

>CYP257A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVRTELQGI PDLSDPETFK EGIPYEAYDQ LRSLPGLPWH PAESGTLNGG 00500051 FWVVTRYDEV VEILQDPARF SSVQGSIYPL PNPTGDGPMT KHILLMDPPE 01000101 HSRVRRAAAK SFGPRIVANF ESWIRDVVVE TLDDALPLER FDWVYEVSRL 01500151 VPSRVIAQIL GIPLEHRGYI VEATNALFES QTVNDGGASL TREFMKIGEF 02000201 MTRLGEEKLR NPADDMTTVL AQSLESGEID LVEYQLYTAS LFAAGFETTH 02500251 TTISHIGHLL ATEPAIRAAT TRALDEGKSA ALVDEFLRYI TPAMRFARTA 03000301 TQDTEFRDQA IKKGDTLLVV FSAANRDPAA FPNPNEFNPF REDPKPLAGT 03500351 GGAGLTFGAG AHRCIGHMLA KLELRILLEE LHVRNIAIEM DGDAQRGASG 04000401 LVNQLLALPV TVAVG

Alphafold2

257A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP258

>CYP258A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VLDPFAPGLS VAEADRLHER LRAAGPVVEA TDADGLRVWI ITRYDDVSRL 00500051 LADPGIVNVR PGGPSGSPGL PLPPALARNL LNMVPDDHRR VRALAAPAFS 01000101 RRRIGAVRQM VTARVDGLLD ELDPNETGVV DLFPAYASPL PADIIAEILG 01500151 VTDTDADEFR TAAATVMAMN PGEPTADDPR IPAMTTIVLV LMRVIAAKRK 02000201 SPGDDVITDW ITARDAEDRL TEEELISLAF LTFLAGFENS VYQIANAIGI 02500251 LAGLDRDQLL VEIADDQLWR ARVKQLVLDA APGSYAIRRF PIDDITVGGT 03000301 RIPAGSPLYL SLRAATNDPA RGERPDLAFG RGPHYCLGAD LAVMQLDIAT 03500351 RHLFQRHPGL SLVKPWETLD RRHTWRTHGP TEMPVNLI

Alphafold2

258A1_m4.pdb

.fasta

Heme Docked

PDBj

CYP259

>CYP259A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLSLGRTDHD SFPTAAGELP VLGHLPVLYR DAIGLLRRSR SALGPLFWVN 00500051 LGFAQRRLFY VGADALDLLR APEVVMDHPE HIKGIVGRSM AAQDGPLHRH 01000101 MRSAINPSFS PQGFAKEAAA AIASAIAAKI DAWLQQESFA VHPAMRDLTL 01500151 DIILRICGVP TGDIRAWSRA YRELFLGVYP IPGRLPGMPA YRSDRARAWI 02000201 DDRLRKLLAL ARGGGAAGSL VEMLSNARDD EGEPLSDQGI VDNLRAIVLA 02500251 GHETSASILA WIVIVLSRRS EVWSALCDEH AAAPDAPVPM SAREADRLPV 03000301 ANAIFREVLR MYTPVWFIFR TVTETITLSG RRIPRNTPLA LSPAQFGYDP 03500351 SLFPDPDRFD LSRWRDRSIP PGPLELAAFG GGPHFCLGYN VACVEALQLQ 04000401 FILVRALRRA GLVPHLEGAS PKHVYFPTGH PTAATRVAFR RC

Alphafold2

259A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP260

>CYP260A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MADEKAPERE HNQGARADGE RSSGAARRVA AFAAALPRLT LHDDLHSVSS 00500051 MDFPLANLFF VPSEDATAFG RRLRAAAQQA PIVFDTAFGM PILLRKSHIT 01000101 TAYRDTATFS TRMFQAGILN GGLAAMQGDE HARMRRVYNM FFLPRAVSQY 01500151 EERFVRPISE QVVDRLAGKP RVDLLEDFAM ELPRRVIGEL FGFPAEKLHE 02000201 TDERVRAMLR GLVRMHDPAA VAESQRAYGE TLGLITEVVE RESRDTSDTL 02500251 LGEILRTLKA EHMDTIEASR QIVLSLILGG YETTSWLVAN TIHALLAHPD 03000301 TLARVRQDPS LLPAAIEEGM RWCPSSFGVL RMVERDVRLD DQALSAGTVV 03500351 CLAGIAGNYD ETAYPSPEVY DIDRKPLPAA NVFGGGAHFC VGAPLARMEA 04000401 RVGLQALLAR FPGLRAVPEE RPSFMYGAKD SVAHGPDKLP VLLH

Alphafold2

260A1_m3.pdb

.fasta

Heme Docked

PDBj 2.7Å

5lib

CYP260A1 WT

PDBj 1.4Å

6f8a

CYP260A1 WT

CYP261

>CYP261A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 METARAEVAP SAGMRDLSTL PGPRRLPLLG NLLEIRPSQF HLTLEGWNRK 00500051 YGDLYTFWLG RTPVVVVGSA ELVQRILRER PKEFRRWSPL QELIDEMGFG 01000101 GVFSAEGDQW TRQRRLVMSA FTAGQLRESH GTLSTITRRL RERWRASAAR 01500151 GAPVDARRDL ARYTVDVTTA VAFGLDMNLI ERGTDPLSDQ LETVFASLNR 02000201 RVFAPFPYWR HVKLPADRAL DQALSEVRTR MLDILRTTRA ELDRDPARAA 02500251 APRSLLEALL VARDADDPKA RLSEQEVLGN VLTLLLAGED TTADTMAWML 03000301 HFMALRPDVQ ARMRAEVDAE LGDADLPESP EHAQRLRYLG AVAQETLRLK 03500351 SAAPILFMEA CADTVVGSLR LAAGTRMILL TRQIALKEEH FHDAASFMPE 04000401 RWLTPRPAGA GKHDPRAALA FGSGPRVCPG RALSMVESAM VGAMVARDFD 04500451 VSLVDPARPV KELLGFTMKP EGLFVRFPPR HR

Alphafold2

261A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP262

>CYP262A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VVGLLPALAR DAPSLLRQLA RQYPGELIEL DLGLTRAYLA THPEQVQYVL 00500051 HDNWRNFGKE GGMWKPIGRL LGNGLVTAGG DEWLRNRRRM QPLFSSRQLA 01000101 GLVDRMFDVV EGDLPRLEER ARAGAVVDMD KEMMQLTQRV ILATMFGVSI 01500151 TPREADSLGE VLLVAIQALN ARMFLYFMPD RLLPGERALR DAIARIDEAI 02000201 LRLVRERRRS KEERDDLLSL LLRARDESGS GMDDRQLRDE LVTMFIAGNE 02500251 TTAITMTWLF YLLDRNPGIE RKLRAEIEEV VGDRRPTAAD LSRMEYTKMV 03000301 IQEAMRMYPP SWLVPRTVKE DDQICGYPVP AGATVILSQY VMHHDPAFWE 03500351 APAEFDPERF TPERSASRPR YSYMPFGGGP RQCIGNLFSI MEAQIVIAVL 04000401 LRRLRMRLVP GHPVSPQAVA TLRPRHGLKM TLHAV

Alphafold2

262A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP263

>CYP263A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSATLERQPP GPGPLASLRY LANFRTGDMR HYEDLFARHG DVVRLRAPGA 00500051 EDFVMAFHPN DIAHVLRTNA RNYPKGKRYH ELAPVLGWGL VNSEGELWRR 01000101 QRRHVQPQFN HANTLKFVPI IVEHMEAVLR RWDAQPGEFE RDINDDMMDV 01500151 TFGIAGEAFF GAALHAHTDT VREAFKYALS IALKRMYSLV NPPLSWPLPS 02000201 HLRFRRAMDR VHAVIDGIID GYQRGAGGED NVLVRLMNSV DPETGAKMDR 02500251 AQLRDEIKTI LMVGHETSSV TASWALYQLA RHPEVCARLT DEIDRVLGGR 03000301 SPTARDIESM PYLTMVFNEC LRLLPSVPFI LRSPLEDDVI GGYKVAAGST 03500351 VAIVPWVTHR HPEFWSDPLE FKPERFANHR RSADDKLAFI PFGAGQRICL 04000401 GEFMGQLEGK IMVAMALQRY RLRLVPGFDP RCRGFISLQP VNGMRMILKR 04500451 REPSSAVRAA PAGREEPAGA EPVVTEPVAA AMSAHGCPYS ARA

Alphafold2

263A1_m1.pdb

.fasta

Heme Docked

PDBj

CYP264

>CYP264A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSERVDIMTP AFRADPYTPY AAMRREAPVC QVDPGGMWAV SRYADVATVL 00500051 RSPERFSSQG FRAAWQPAWV GHNPLASSIL AMDGPDHARL RGLVSRAFGA 01000101 PAIARIEQRA RDLCERLAGR LDGEVDFIAA AAAPLPAFVI SELLGLDHAL 01500151 EPHFKRWMDD LLSVTPEPAS AEHAARVRAT IAELDRYMAD VIAARRRSPS 02000201 DDLVSELARA GELLGDREII DLLVSILGGG LETTTHFLGS SMLLLAERPA 02500251 ELERLRASPQ LIPRFIEEMM RYDGPTQSVP RLTTSDVALA GVTIPAGSLV 03000301 LALVGSANRD EVRFTDPDRF DLHRGQPSLT FGHGAHFCLG AALARMEAKV 03500351 ALEVLVPRIG EVTRAPGEIP YNRTLTVRGP VSLPLRFRPA

Alphafold2

264A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP265 !!!FL!!!

>CYP265A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSTPHTLQPD DLMEEGGDVV RRAQEACWYA ETSMGKAVLR YREVHDLLRD 00500051 ARFRNMGTDV LTRQGVTEGL VRRMYEGFLL STEGAAHARL RGLLKKGFTP 01000101 DAVDALRPRM RERMHALLDE IGDRTEFDFV TAVAERFPGQ VIIELLGLPV 01500151 SFEDPDFRRW CSDLGYIVGL EVKKHLPRLE AAFEGLRGYI EPTIAARRAE 02000201 PRGDLISTLA AAEQNGDRFS EEELVCQIVA LLFGGLDTTR RTIGKAFLVM 02500251 LEHPDQRRLL QERPDLAEPA AEEILRYAPG TRVAVRVATC DLELHGVPIR 03000301 AGEMVIAATG AANRDPRVFE APETFDITRR GAAPLSLGAG PHHCLGAYLA 03500351 RAELQEAVPI LTRRLTEAAL ASPVVVRTAR EGSPGPRVIP LRCRVRPR

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP266

>CYP266A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VETQVAATQP EINIIAPENL RWPYPMFRRL LLEEPVFYDK NTGSWVVSRY 00500051 EDVNALLRDA RMSADRYVAL ADTVPPEQKE MNSYIVKSLS MFMLNVENPT 01000101 HFRLRNLTNR SFTPKSIAAM RPSAHAVVNE LLDAVQPRGH MDVVADLAYP 01500151 MPIKFICGIL GMPVEDMGLI KQLSDDVSVY IGSAGKAAGC IPPAYHAIVE 02000201 FSKLFRPLVE ARRKEPKDDL ISSMVTTRVD GDSLSDDEVI ANCILFLVAG 02500251 FETVTNLIAC GTLALLEHPD QLELLKRDSR LMEGAIDEML RYYPPVNRTA 03000301 RLCVEDIPLR GKVIKKGQIV VLMLGAGNRD PSEYPDPDRF DIARENRSRP 03500351 LSFGGGHHFC IGSHLARMEG EVALGALLQR MPNLRLATQE VEWRGNSRFR 04000401 GLRALPVSF

Alphafold2

266A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP267 !!!FL!!!

>CYP267A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNSPDAPKPD APPAANPAAD ADLDPFRLQS PETLANPYPV YARLRQEAPV 00500051 YFSAAYNGWL ITRYDQVAAG FRDPRLSAKR SSAFVTKLPD EVRQRLEPLR 01000101 RNLASWALLL DPPEHTRIRS LINKAFVPRL VEGLRSRVET LVNELLDAVA 01500151 PAGRMDVLRD LGDLLPLLVI GEVLGVPAED RHRLKGWSNA LSGFLGAGRP 02000201 TLEIAGGALS AVAELEDYFR GVIAARRQSP GNDLLSQLIL AEEQGMILGE 02500251 QELLSTCCML LFGGHETTKN LIGNGLLALL LHRSEREALR ATPSLIGPAV 03000301 EELLRYDSPV QWMSRVALDD IELDGVRIPK GDRAFLVLGA ANRDPAQFPD 03500351 PDKLDFRRTD IRHISLGLGV HYCAGSALAR VEAQAAISTF LRRFPDAELS 04000401 PGPLTWRMNP GMRGVTALPI ELGPQSSAS

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP268 !!!G Insert!!!

>CYP268A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTGVIAERIG IMAVLTGESG TDRSGRPYDE IDLSSRAFWS GTAAERERSF 00500051 AVLRAERPVS WHPPVEDSLL PDPTDPGFWA VTRRADIVTV SRNNDVFLSG 01000101 HGVMFESIPA ELLEASQSFL AMDPPRHTKL RKLAHAALSP RQVRRIEDSI 01500151 KANAKAIVEE LRSAGSGCDF VDHCAKELPI RTLSDMMGIP ESERERMAHA 02000201 TDALVSWADP EFLNGRPALE VLLENQMYLH QVVGDLATQR RERPGDDLIS 02500251 SLVTAEVDGD RLEDAEVAAF FVLLSVAGND TTRQTISHTL RALTVFPDEK 03000301 FWLLEDFGHR IGTAVEEFIR WASPVMTFRR TAAADVELGG QTILAGEKVV 03500351 MFYPSGNWDT EAFDHPERLN LGRDPNPHVG FGGGGLHFCL GAHVARAQLR 04000401 AIFSELFRQL PGIQAGEPTY LAGNFVHAIR AMPCTF

Alphafold2

.pdb

.fasta

Heme Docked

PDBj 2.0Å

6bld

CYP268A2

CYP269

>CYP269A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAYPETNTAT DYPFTQPDAL SAPRDLGKLR QRCPVADVRF PSGDPAVVVT 00500051 RYDDAKRVLD DPRVSRNMYR ADAARLSTTQ LNMLELEFVS TLVDPPNHTR 01000101 IRRLFAKALR RGRIEELRPR IVTIVDELLD AMLAGPMPAD AVDALARPLP 01500151 RQIIGEMFGF PPGEGDRAQY WAERLFSLSL HTAEEMAAGQ QEFAAFICEL 02000201 AEKRRAEPTG DFFSDLVAVS DVDDGQLSHL ELVYLAQALF AAGIDSTWVM 02500251 ISRMIGLLLH KGCYGRLVAD PKLIEPTVEE VLRYLPPSNL GALRYAVEDI 03000301 ELDDAVIPKG TTIAVSTTAA NRDPRFFDDP DEFLVDRSEN RHVSFGHGRF 03500351 LCPGAWLARC EMQSLLRGLV TRVPTLELAA DVNDLKVRTG MMNEGMLSLP 04000401 VRW

Alphafold2

269A1_m5.pdb

.fasta

Heme Docked

PDBj

40.66% to CYP286As

CYP270 rename to CYP190

>CYP270A1X=CYP190A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTKPKLVFDP YSEDYFNNPY EIYRRMREEA PLYYDEKEDF YALTRHVDVA 00500051 AAFKDYETYS SARGCDLAMV RRGISPEQKS IIFMDPPEHR HMRSLLNKAF 01000101 TPRAIQSQRE TIIEVVDKYL SAADPDNFDV VQDFSGPFPV EVITRMAGVP 01500151 EEYRQQVRHW IDTSLHHEPG QIEVSEAGMQ ANIDTAMYYF GLVQERRQDP 02000201 QDDMISRLIA AEIPGENGQM RKLDDIEITG FATLLGGAGA ETVTKLLGNA 02500251 AVIFARHPDQ WQKLQEDRDK IPGAVEELLR YEGPVQYNVR YTLKEAHVSG 03000301 GVIPAGKPVF LCGAAANRDP EAFTDADTFD IERDQTEAQH LGLGYGIHSC 03500351 LGAALARLES RIALERLLDF MPRYDVDWAG CRRVTMQNVA GWKNVPVKVL 04000401 R

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP271

>CYP271A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEGNERFVPR LAPLTLAVKS LANPQFIYNA KAQGVRAERT EAGVVTLYRR 00500051 DDLMRISRHP AVLGPGGRGG NFGNDNPLIP LEIDGEEHKK WRRLLDPLFA 01000101 PKQMARLEAA VRNRAAQLVD GFASQTSAEL YQAYCAPLPC FTFLELLGAP 01500151 LEDIEFFTAF KNGVLHPQGE TREEIQANQA AAGAMLLEYF TRLVARQRAE 02000201 GAQGEEVLAV LLRSRIDGQP LTDAELFNII HLLIFAGLDT VTASMSCILA 02500251 WLGQHPHERR RLVEDTARIP AAIEELMRYE SPAPSGIRYA TADIDLGDGL 03000301 TIRAGEAIHV SWAAANVDPT AHPDPLHVDF DRARFHHLAF GSGIHRCLGS 03500351 HLARLELRVA LEEFLARIPD YAVDTAGLVY DNVSVRTVQQ LRITFNANTP 04000401 SPVDPSQRHA FMAPLTGQRD SVLERNGHEH R

Alphafold2

21A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP272

>CYP272A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTEAGGFVDQ TVTGVAEPQP MYKALRESNP VFRSTQAVVL SRLADIEMAL 00500051 KHTELFSSNM DAVDLGNVRP LIPLQIDPPD HAKYRRILDP LFTPREMARR 01000101 EPLVTELVNE MIDRFAPRGE CDFHAEFAVP LPCTVFLQLL GLPLEDLDRF 01500151 LLWKDGVIRP AGDSGFDRRH ESSAGVAQQI YEYFDKAIDE HIAVPRDDVL 02000201 SAMIAADVGG QPLSREELLD ICFLFLIAGL DTVTDSLDCF FVYLARHPQH 02500251 RRQLVERPDV LPGAVEELLR WETPVPGVAR VATQDVEVGG CPISKGERVS 03000301 PLLGAANTDP AEFPDPEIVD FTRSPNRHRA FGGGPHRCLG SHLARMELRV 03500351 ALREFHRRIP DYEIRPGTQL TYTAALRSVE SLPLVFPVR

Alphafold2

272A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP273 rename to CYP187

>CYP273A1X=CYP187A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MMTNVPTAAG DETVSLRDPY PFFARKRREA GVFAGTVMDY SKTPESLMPK 00500051 QEYSAVSFDA VNTVFRDGRV FSSKPYDKTI GLFMGPTILA MEGKKHRDHR 01000101 NLVSAAFKSK ALARWEPTIV RPICNALIDD FIDAGTADLV RQFTFEFPTR 01500151 VIARLLGLPD EDLPMFHTRA VQLISYHVDY ERAFEASAAL KDYFLEQIEQ 02000201 RKSKPTEDII GDLVTAEIDG EKLSDEAIYS FLRLLLPAGL ETTYRSSGNL 02500251 LYLLLTHPDQ FAALQADREL LAPAIEEGLR FETPLTTVQR FTTEDTELQG 03000301 VRIPARSVIG VCIGSANRDE RRWERSEEFD IFRKHVPHIS FAAGEHTCLG 03500351 LHLARLETRV AMECLLNRLT NVTLLSDGDP HIHGQPFRSP TALPVTFDAK 0400

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP274

>CYP274A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVAGSPTLR TPPVAPGRLP GLGHLSALAR DPLGYLARLR SVADVVTIYL 00500051 GPRPSYLVTS HDLVRDMLVS QSECFTRGAV FERAGKALGQ GLLVSDGEFH 01000101 RCQRRLLQPA FHHRQTPRYA EVMADAAQTL SRAWRPGVPI EVTHEIHTMA 01500151 LAVVCRGLFH TDLGGSAVSR VERALTGINQ GVIWQTLYPF DWLARLPIPV 02000201 NRRFRSAITD VRDLVTQLIS ACRTTGEGRE GVLAMLLQAR DEETGRPMSD 02500251 DQVRDEVLTL LLAATETSST TLSWLFYELD RHPEIERGVA RELDDKLANQ 03000301 SITHDNLRDL DLMKRVVDET LRLHTPNSIL TRRAVREVQL GQFRIPAGAE 03500351 VAFSPTAIHR DPTVYDDPLS FKPDRWLSEN SAHVPRHMFI PFGMGKHKCI 04000401 GDSFAITEMH VTAATVLRDW RLSLAPNAHV RERPWAIVQP HGLRMIPFPR 04500451 R

Alphafold2

274A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP275 rename to CYP189

>CYP275A1X=CYP189A6---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVMTGTSAIE LYYDPFDSGI DDNPYPVWQR MREEAPLYYN EKYNFYALSR 00500051 YEDVARELPN WQTYRSGRGT TADILFSNVE VPPGILLFED PPLHDLHRRL 01000101 LSRVFTPRRM LAVEDLVRGF CVRELDPLVG AGGFDFIRDL GAMMPMRTIG 01500151 YLLGIPEEDQ EKIRDRSVAN IELSRDSDPA AVDANVFANS IALFADYIEW 02000201 RADHPSDDLM TELLRAEIDE PDGTRRPLSR TEVLAYTAMI AGAGNETTAR 02500251 LIGFMGQLLS DHPDQRRELA ADPSLIPGAV EETLRFEPPS PVQARYVARD 03000301 AEHYGRVVPE GSFMLLLNGS ANRDPRRFTD PDRYDIHRQG GGHLSFGQGL 03500351 HFCLGSALAR MEARVAFEEV LKRWPDWEVD YANAERARTA SVRGWARLPV 04000401 VTGG

Alphafold2

189A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP276

>CYP276A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDTMLARFD PHDQQLRDDP YAVYATYRQA DPVHWGQPFK PGDNGCWYLF 00500051 RHDHVVAMLR DPRFRRKLVP ADPLASGRVF GAPRGFAELS GRLLLSLDPP 01000101 HHGRLRSIVA PTFTPSAIES YRMIAAKAAD RLIDGLDEAT AFDVIDNYAV 01500151 PLSMTLIAAM LGLTADELHP DLPVWVSRFG DGFDLRKEPA AIARADDAAA 02000201 NMLWFFETAA ASHDRLRPGL ISALIAAREA GQLTHEELLA LCVQLMFAAH 02500251 GTTVAQIGNM IADLWTRPDQ LALLRAHPEL MAAAVNESLR FNGSVQSTAA 03000301 RKPIEDVTLG GAHIRAGQPV IAFVGAANRD PKVFEEPDRF DITRARGAAV 03500351 MFGAGIHYCL GARLARLECQ IAVGTLMRRL PRLKLTERTQ LHRHTNIVLP 04000401 ALLHLKAVTA A

Alphafold2

276A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP277 rename to CYP185

>CYP277A1X=CYP185A3---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSVAEPKRDI AGLPLAPKNP LSYRERLRAI KEFHTGTNKL RDAGGPVTRV 00500051 TLGPRWLISP IVLATSPQGI RDIVSVRDGS IDKTSTVATE LRRLLGPNLF 01000101 VLPHTEWLPR RRTLQPVFTR QRVREFGGHM AEAAESVCAG WPEDTEIDLD 01500151 AQCRTLTLRA LGRSVLGLDL DERSDAIAEP LRVATSYAVR RALRPLRAPE 02000201 WLPTPSRRRA RAAAGAIRAL ADEILQACRA DPGREAPLVH ALIAATDPET 02500251 GQALSDKEIR DEMIIFLFAG HDTTATTLTY ALWALGRHPE YQARVAAEVA 03000301 ELPDRHLTPD DVARLGFTVR VLQEALRLCP PGPTGTRMAT RDVEVAGYRV 03500351 EAGTMLAFGR MAVQTDPSLW DAPLRFDPDR FDPRRAGDRD RWQYLPFGGG 04000401 PRSCIGDHFA MLEATLALAT IVRRVEIESL SDDFPLAVPF TMVAAAPIRA 04500451 MVRRRR

Alphafold2

185A3_m2.pdb

.fasta

Heme Docked

PDBj

CYP278 !!!FL!!!

>CYP278A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSTETVSGTV PAVELLTTPQ GIANPYPLYD ELRGLSPVAG YRDWPPGTVP 00500051 GADEPVTAWA LFRYDQVFAA ARDAATFSSR DPLQEASSAP SLMLVNTDPP 01000101 KHEVERKLVS QAFSPRRVKR LEGWLDDLIP QLLNDLEKDS RDGAEDGSPD 01500151 GVVEVMGFAA EIPTRAMVRL LGLPDGDHVR FRRWANAFML SSSLTPEERM 02000201 ASNQEMVGTF ATRLAEHTAR LAEREASDDV EDAEDLISAL LRAEVDGQRL 02500251 TPEEIVRFCV TLVVAGSETT TFLIGNLLHA LAREPEVQAR VRADRTLLNI 03000301 FVEETLRLDG PPQRLFRIAT RDVEVGDKLI RKGEWVALFF GSANRDPAVF 03500351 PDPGRLDIDR PNIRQHLSLG HGLHFCLGAS LARLEVLAVL NAVLDRYQKI 04000401 ALTDDPGTKQ TASLLTHAFV RLPLHLS

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP279

>CYP279A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVGAAPPSV FDSDLPTLHY HSDETPAQVY PRLREAQRRA AVAIGPHGPE 00500051 VLSYHLVRSV LRDPRFQIPP GINLLAQGID SGPLWDKVAN SLLCLEGDAH 01000101 HRLRSLCSKA FTPRTVARLH DTMAAVMNEL VDRVAAAGRC DVVTDIARPY 01500151 PVPIICALLG APREDWRRFS SWADDVFKAF SFTVDLREVE PVVMRAWREL 02000201 DDYVDEMVAR RRHNLTDDLL SDLIRVGDEG DRLDAAELRM LAGGLLLAGT 02500251 DTTRNQVAAS VQVLCEHPDQ WELLRQRPEL AMRAVEETMR HSPIACGTLR 03000301 LVVEDAELDG HLFPAGTAVL VNTFAANRDP VVYNDPDRVD ITREAAPPIL 03500351 TFGGGVHYCL GANLARREIA EALNVLANRL RNPRLAGPAP WKPMVSLSGP 04000401 TSLPIEFDR

Alphafold2

279A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP280 rename to CYP191

>CYP280A1X=CYP191A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDFAYDPFDA EVMANPLPYY RILRDHHPVY YMPQWDTFAL SRFDDIWRVL 00500051 EVNNGTFVAS EGTLPPASVL AQHNDGPVDD PPLHPLPFHA MFDADLYGEI 01000101 RRTHSRPFRP RAVTDLEGRI RTLANERLDE LLARGSFDLT QEYGGVVVAT 01500151 IVCELLGIPT DLAPQVLAAV NAGSLAEPGV GVDTGQARPN YFEFLLPAVQ 02000201 RRRADPSGPP LEVVDGLLGY QLPDGSALDD LEVATQMLCI FIGGTETVPK 02500251 IVAHGLWELS RHPDQLAAVR ADPQHNIPVA REEMIRYCAP AQWFARTVRK 03000301 PFDIHGQTPN PGQRVITLLA SANRDEREYP DPDDFVWDRP IRRSLAFGRG 03500351 QHFCIGYHLA RLEVAVLLQE WLRRVPDYAI RADAATRLPS SFQWGWNKIP 04000401 VEV

Alphafold2

191A2_m5.pdb

.fasta

Heme Docked

PDBj

CYP281 rename to CYP188

>CYP281A1X=CYP188A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPDAWTDTYG GHRVAAGSHE VFELARCPAV SNDHDINGER RGYKGISIPT 00500051 ASRVSAVRGG ILEMDDPEHR IYRTVLNPYL SPAAVKRWEP FIDEVTRAAL 01000101 DEKIEEGSID FVDDLANIVP AVLTLAMLGI PLKKWKMYSE PVHAAVYTPE 01500151 HSPDIERVTA MHREMGLDMV NNMLEIRENP RPGIVNALLQ MRIDGEPAPD 02000201 LEILGNLGLV IGGGFDTTTA LTAHSLEWLS EHPEQRQLLS DERKTLLDPA 02500251 TEEFLRYFTP APGDGRTFSE DFELDGTVFK EGERLWISWA MANRDPAVFH 03000301 DPDEVILDRK GNRHFSFGLG IHRCIGSNVA RTVFKSMLIA VLDRMPDYRC 03500351 DPEGTVHYET IGVIQGMRKL PATFTPGRRI GAGLDETLEK LQRICDEQEL 04000401 ARPITERKEA AVID

Alphafold2

188A2_m5.pdb

.fasta

Heme Docked

PDBj

CYP282

>CYP282A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDVTKSIDEH VKHFDIRDTA FDDPELVYKV YEAMRTIGAI VPTDQLDFSG 00500051 RFKETLQVIG YAECHQLLKD WRRVSNDPRK AFDNLSEIER RSDLPVDDSA 01000101 TPISTDPPVQ KELRKVLSPS FTPERMVALE PKVRAVANRL IDEFIEEGKG 01500151 DLADFSWKLP SEILFAEFLG FPAELLGEAL AAQGASLLAS DAAERDQSAA 02000201 RVFELIADVL KERVGQPPRD DIIDGLLNSE IGGRPLTVGE RMANATLLVA 02500251 ASLETTSNAL SAAYYWLAHH PAERSRLVAD PSLLPAAVEE FVRYSGSVHG 03000301 LPRYITADVE ICGYGFKRGD AVIVNYAAAN RDENEFADAG KCIIDREPNR 03500351 HLGFGFGTHR CLGAPLARLE LRVGFSEVLR RLPDYRIANE DTCEFSGSSF 04000401 TRGYQALPVA FTPGRREHS

Alphafold2

282A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP283

>CYP283A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDFDFFAAPQ QYRDAAAAHA GEHGAFYSDR GFWVLTTYDG IVDAFKDEGT 00500051 FTVGRVSAAE GAEEERWIPL TVEGREHTAW RQRLGAWFTP QRVRELTPSV 01000101 RAGARRRIEE FLDKGEVSFN EDFARPYVLD NLMTAVGWPP DAFDLLLAID 01500151 RAMIDSRSAP DPRVAAYGEL GLPALERFAR EHVARRRAEP ADDLTTASFG 02000201 WEIDGTPVSD DDRASLLCTL FLAGVDSTVN HLANAVQHLA HHEEDRRRFL 02500251 AAPEARPPAV EEFLRVNSCM YPGRQAATGG AGGVADRGDT VLLPLALANH 03000301 DPEVFPEPGR IDFGRPRNPH IAFGTGPHQC LGAAFARAQI LVALEEWHAL 03500351 VPDYAPHPGQ RATEPRFLRN SYDLRLVW

Alphafold2

283A1_m5.pdb

.fasta

Heme Docked

PDBj 1.5Å

7abb

CYP283As

CYP284

>CYP284A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLQYITAQID NSSSFPYLVT VLSVTTIAGT FAWRWWKQKK KYKSLQSLPS 00500051 PPKHWLLGNL PQVLAAVKQK KLFQLFFDWS QQLGPMYVVW NGSSPVVILS 01000101 KPKVIEDTIV NGMRDGSLIR SARLRQAWND ISGPILIGET GNEWQWRRKV 01500151 WNPEFSSSSL AKYLKIINQA CVQVIDTLKE TALPKEVEVD PLFVELTMRV 02000201 ISSLVLGIPV DRKITTNEGP PLEVLKVYEA MCVVGYRFLR QATGEKIWMK 02500251 YLPTKNSQDY WASRRYLEEF LTPRVDLALQ MREQSTDFPQ VSPLFRESML 03000301 VRIAAKEPKY NRQTLIAESV EFLIAGTDTT AHTLSFAVGE LSLNPRVFQK 03500351 AREIVDQAWQ GQDNINTESF KELAYISAIL KETLRLYSIA SGSTSLEAQR 04000401 DTVIEGKVIP SGTRISWSML AAGRDPEVYA NPEEFLPERW LDKSKETSSL 04500451 PMIDFGSGPH RCLGEHLSML EGTMMLALLL RHFDWELVNG RSSLEQLQQN 05000501 LLIYPSDKMP VRFRLRN

Alphafold2

284A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP285

>CYP285A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKLSQSVPPE QIDLDSVDLT DSVLYGEGDP HSIWNAMRQR DPVRWQQVDD 00500051 TLGFWSVTKF EDADLVLRDH TLFTSQRGTM LFLLGKDDPA RGRQMAATDP 01000101 PRHTRMRAPM QRALTNKQVE KYREAVTGEV RRLLEPALSE EPFDFAESMM 01500151 TLPMTAAGTM MGLPREDWTR LTQLTTMSIA PDDPEFAGPG SAEETLKAAH 02000201 RELFAYFHDI LRDRRNNLGD DLISLLLGME IDGRSLETGA ILSNCYSLIL 02500251 GANVTTPFVP TGAMAEIVGT PLLHEWRTDP KLMNTGVDEA LRWSSPTNHF 03000301 MRYALQDIEL RGKKIRAGDA VVVWLGSANR DEEVFANPFT FDIRRKPNRH 03500351 MAFGSGPHYC VGHTVARMSM KILFTELFES FEDFELVGEV EHLHSNFVAG 04000401 IKHMPMVAKV RAGAARSHTG LVK

Alphafold2

285A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP286

>CYP28A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVPAPPFLGH AAEMGTIKLR PFLTRCYQAY GPVFQLTVPG QKITVLAGPE 00500051 ANLFAMKEGH RVLRSLEAWR DNDHEMGSDR SMISLDGAEH RAYRRVEGRA 01000101 FARSFFAAGL RPALAVLAED LAPFQPGDVL PVATWCKKTI TEQLARMAVG 01500151 GTVRPYLPDL LHFIQTALQV TVNRQLPPAV LRLPKYRRAK ARIFQMVDDL 02000201 IEDHRQNPPE KSGRAPDLID DVLADQQVNP ERWEHPDVRL AALGAFIAGM 02500251 DTAANSLAFV LYRMHLHSEF LPALRAEADA LFRDGPPTAE ALGRSPLLHR 03000301 FVMETLRVHP IAPALSRTLT EDVEFAGHRI PAGTPVIIGT TVPHGLPELF 03500351 PDPEHFDPGR FAPGRAEHRQ PGAYAPFGVG SHTCAGSGMA EGLIMLGAAA 04000401 ALRTLDLSLE PDYVLRQTAK PTPSLDNKLQ LRVNAVRHNP VFLVH

Alphafold2

286A1_m6.pdb

.fasta

Heme Docked

PDBj

CYP287

>CYP287A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLSSLHDLPE PASRPGSGHL QDWAARPLAL IEEGATQALA AGQDLFRLRL 00500051 GLPAVVGLSP AWNRRVLTDL NTFVSAGSFS AVVPYLAGGV ILTDAPGHGA 01000101 RRRALNPGFG KGSVQQLRER MRQASSPVPT GRFNALAWAD ETVRRQLNAA 01500151 YFASEFDDRL LAAFLAPLRR PFPVPALPRP LLFRRVEQEI RRLAERRLRE 02000201 GGDDLLSTLA PLPGGLLETR ISLAAAHDTT THALAYAVWE LAKAPQDQTP 02500251 HTHSAVLKEV LRLYPPGWMG SRRLSRAAEW QGTEIPRGTL ALYSPYLTGR 03000301 DPTLWERPLD FRPERWEKSP PAWAYLPFGG GERTCLGVHL AQTLILDVLA 03500351 ELPPLQAHWG NDEPHPGITL GPRGPLVVER R

Alphafold2

287A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP288

>CYP288A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIHNMETRCG SYHRATGILT GDDMTSDSTT ALSTAPETTS GGCPYGHGNP 00500051 DATGTPGTSH HGYEPFNMTN PFPAYEELRR EEPVMFDERI GYWVVTRYDD 01000101 IKATFDDWET FSSENAQAPV RKRGPQATKI MEEGGFTAYS GLSARIPPEH 01500151 TRIRAIAQKA FTPRRYKALE PDIRANVVAR LETMLKQGAP ADIVPALAYD 02000201 IPTITILTLI GADVSMVDTY KRWSDSRAAM TWGDLSDEEQ IPHAHNLVEY 02500251 WQECQRMVAD AHANGGDNLT ADLVRAQESG QEITDHEIAS LLYSLLFAGH 03000301 ETTTTLISNC FRVLLAHRDQ WEALIEDPKK IPAAIDEVLR YSGSIVGWRR 03500351 KALRDTEIGG QPIKKGDGVL LLMGSANRDE ARFDDGETFD ITRPNAREHL 04000401 SFGFGIHYCL GNMLAKLQAK ICLEEATRLV PSLELADDQS VEFRENLSFR 04500451 VPVSVPVTWS N

Alphafold2

288A1_m1.pdb

.fasta

Heme Docked

PDBj

5xjn

CYP288As (P450CREJ)

CYP289 !!!FY!!!

>CYP289A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRAGGIMTSP TERPKQDWDP RSEAVLSDQI GAYDAMRHQC PVAHSDYLGW 00500051 SLFSYDDVVR VLDDHETFSS VVSAHLSVPS GMDPPQHTAF RQLVERYFEP 01000101 ERIKAFEPIC REISKKLVCE LPRDAEIDLV TQFAQLYAVR IQCAFLGWPD 01500151 SLQGPLLDWV HKNHAATLAR DTKAMAAIAL EFDEYIRDLL DERRKLGADA 02000201 PDDVTTRLLR DRIDRRNLTH EEIVSILRNW TVGELGTITA CVGILCHYLA 02500251 KSQQTQALMR GGPDLLPAAI DEILRLHAPL ISNRRVTTRA VVVGGREIPA 03000301 GEKITLMWAS ANRDEAVFDK PDELRLNRDP ALNLLYGRGI HVCPGAELAR 03500351 AGLRILMEEL LGQTRKLDLV PGSVPALAVY PASGFSRLPA RIS

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP290

>CYP290A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDLISQTVVD GKAGPGAPPT YPTLKDVDLA DIFRFTKGQP WADFARMRQE 00500051 APVMWHPEPM GGPGFWALTR YEDVHRVNGD PETFSSQRGG ILMSMGAPEK 01000101 RHALLFRASM DTMINMDAPH HLQLRREHMP YFTPSYLRGL TERVKGEVTR 01500151 LLDEMEPLLA NGAEIDMVEH FSSVLPLFTL CEILGVPPED RPKFLTWMHY 02000201 LERAQDLAVK QANAPMQPTL ELMQFVMDFN NNVEEMFEYG RTMLHKRRED 02500251 PKEDLMTAIA RAQLDGAVLP DEYLDGSWLL IVFAGNDTTR NTLSGAMRLL 03000301 TEFPDQKQKL IADPSLLGGA VDEFIRMVSP VVYMRRTATR DVEVNGQLIR 03500351 EGEKAIMYYG AANRDPAMFE NPDQLDVTRA NAGKHIAFGY GPHTCIGKRV 04000401 AQIQLEEAYR QILARFPDLN WTGNIEIAPN NFVHAISKLG VKRG

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP291

>CYP291A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSRLSNPLHS PAFYAGDPFP VYRELRATDP VCWNDEHEFW ALLKYEDIRY 00500051 VSTNPALFSS ARGVTVPDPA LENPVMEGSL IFTDPPRHRQ LRKLINSGFT 01000101 RRQVAILEPK LRAFARAALD AIDPSATEFA EQVAAPLPTR MIAELLGAPD 01500151 EDWEQFRTWS DATVGMDDPD VELDTFAAMG ELYQYFEAQI ARRRDGTLRD 02000201 SDDLLSVLVA AEVDGVRLTD QDLLQFCLLL LVAGNETTRN LVALGTLALI 02500251 ENPDQFRLLR QRRDLVPTAV EEMLRYASPV ANMTRCATRD VDIRGRSIRA 03000301 GQYVTMLYGS ANRDKDVFGP TSEQFDITRN PNPHLAFGCG EHSCLGAQLA 03500351 RLEARVLFEE LLNRFDHIEL DGDVLRMQAT MVPGVRQMPV RLSATAAEPR 04000401 ETGTAAVSV

Alphafold2

291A1_m2.pdb

.fasta

Heme Docked

PDBj

CYP292

>CYP292A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTRTPERHAE NWDLRHPDFN DNDFLYDVYA VMRAKSPFAR TDNPFLSATP 00500051 AGAWVAVRYA ECVQILQDWE HFSSNPTPEG AEQLAGDLVI TLDPPRQQKF 01000101 RKVLNPYFSP ARMKALRPEI GVETDRLIDD FIETGSGDLA AVAWRQPGIV 01500151 FFKYLLGMPV DDVPLCVELT DTALNGATEQ DRMAAWGGLY QHLHDAVTAR 02000201 RGQPPRDDMI DVLLSAEIDG EKLAFADVVS NAMLLVQAGL ETTASAMSFA 02500251 YHYLATNPAE RDRLIDDPDL LTRAVEEFIR FAGSIHGIPR TVAREVELSG 03000301 CTFSPGESVI VNYAAANRDE QEFPDAHRCI LDRRDNRHLG FGAGVHRCLG 03500351 SNLARLEFQV GLERVLTRMP DFALSPTSKA EFHGNSVTRG FRSVPVVFTP 04000401 GKRVTA

Alphafold2

292A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP293 !!!FL!!!

>CYP293A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VDVFDRNFGY DSDTLAAARE RHWYARIPNG LMVLGHEEVN DLLRDHRLVS 00500051 DGKRYLEMHG VTSGPLYDWF VPMILHREGQ DHARLRSVVR RTFTPRVIEG 01000101 LRPYMRETVG RIADGIAETG ECEFVETLAN PFPVMVMGRL LGVPPQDHEL 01500151 FHGWSTDVGL VFSLAYGGDV RERVERAVSE LGDYFDGLIE RRRAEPADDL 02000201 ISSMIAAQTE EEVISGEELR NLLVALVSAG HDTTRHQFGS AMLTLCRNPE 02500251 QWTAFRDDPD RAVEEILRWC PAAPVLFRFA QEDIAYRDTT IPAGTFVMLC 03000301 VHTANRDPKR FDRAEVFDTT ASRQTPHLTL SAGPHFCLGA AAARAELSEA 03500351 FTVLSTRFGA PRIAGEVTWR NPVGIYGPET LPVRFV

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

40.6% to P450 AurH (3P3L)

CYP294

>CYP294A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEQASTGQLG PLPEFLRQAT DEVERIVAPS GDKVLLVRGY ALGRQVLTDK 00500051 RFSRAAAVQP DAPRFNDAQP VPDSMMSMDG AKHARLRRVV AGTFTTGRVS 01000101 AMAPAVERLV DEHLNRVADI GPGADLVEEL AAPLSLSVLC SLLGIPLDDS 01500151 ARFREWVEVL FDITASTPRE KARHRLELIG YMTDTIEQKL QRQDDDLLTT 02000201 LIRAHTQGKM SRPELLTLGL TLLMAGYETT AGQIGLSVLS LLSDRETHDE 02500251 LRAHPELLPD AVEEFLRLTP ATPLSFPRVA VEPVELNGVT IQAGEAVVVS 03000301 LLHGNRDTEV FAEPGQLMLR SRDAVHLTFG HGVHRCLGAP LAKLQVQLVL 03500351 GRLLERFPAL RLTPGPDSVV WKDGLATRGL SRLRVEW

Alphafold2

294A1_m3.pdb

.fasta

Heme Docked

PDBj

39.8% to P450 LnmA (4Z5P)

CYP295

>CYP295A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLGTRTVRPP GPKGRWLVGN TLDYDRDRMA FLVRCRQEYG DVFSFDERTI 00500051 VVLDPGLVHD LLTRTNDDFH SESVPLATRI DPERAAADAQ AWMTARRKGW 01000101 HGLNRAVAQA HAGRLRLLFE QTLAETRGET VDVLPVMERF AGLATADFCL 01500151 GADADGMAEI LTENVRAIEP LGGSSQLFPA WWPSRHIRRF LRAREGTLNA 02000201 VTERIHRRRD KPPADHPQDL LDVLLAAGEP ELTEQQVQLL LRGIMLAAFG 02500251 VPATALTWLV WTLASRPDLH RRVAEEAAAW PGEEAPPLSA LPQTEAVVKE 03000301 VLRLWPPTWL IGRTARRGTT LGEWQLRPDD HVMFSPYLMH RDPRWWTDPD 03500351 ELTPDRWLDP ARTPKRHTYI PFGAGPRVCV GTQLGMIQLC LAAHWLTRNH 04000401 EIRPTTETCA PKFHDLLLPQ GFRARFTPRT P

Alphafold2

295A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP296 !!!Proximal is strange!!!

>CYP296A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLEQDVLDEV TNPFPGLAAL QEQTPAVRVR TPDGPPAWLV TRYADVRDLL 00500051 RDDTRLSVDP DHGAGEDYAG FELPPQLRPH LLAVDAAQHD RLRELISSPF 01000101 TGTGLHRTVE VVEAEAHRLV AALAPDQPVD LVAELAFPLA LTAVRAVLPL 01500151 PDPAHQAFAE WAHDALLAPR RQEDGAEVRA RDTLGRMAEI IQTATTVPGD 02000201 GLLGELQAAY HHGRLSAHEL AAQVFYLLFV PTEPLVDAFG VVLLRLLAGT 02500251 RHRAVLRDSP AARHVAVAEA LRFDTPQGLA APRFALVDLD IDGVRVRAGQ 03000301 TLLLSLAAAN RDPRQFDDPD ELVLTRSPNP HLALGRGDHA CPATALSYQL 03500351 LSSSVAALLS RYPSTTVVPE KTQWRGNFRH RGPASVWADL KP

Alphafold2

.pdb

.fasta

Heme Docked

PDBj

CYP297

>CYP297A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAGPVLSSSD IPGPPPSAGG HNLRDIAEAG GLPGFQVRLH RTYGEIAHFA 00500051 MPNAQVVSVA SPEVLESTLD LPECPMELAP FLTPLADAAN VLLSGEQDRA 01000101 RWRRTVIPAL SCPHRAESDH PRFIDIIEES AQRWSSTGEP VALERELDTL 01500151 ALRLMCTSVL GSTPGQAAIV ERVTDAFERL PEFFLGQVYG VRTTSQEPAV 02000201 DSALTTLRDV VTFLVDEVAP SDLISAARRA GQSSKDLVNT LLAILIGAHR 02500251 TTGTVVSHAL RQLMRHRDVA KRLRAELKTV LGGKAAPAFH ELPCLRYLRR 03000301 TLNESVRLAS PVPGIARRAP DGIALGAYRI PAGTVVHYAI RAVHMNPRVW 03500351 PRPDEFDPDR FDTAPQNSRP AMAFVPFGIG RNDCPGALVA MEHALLMLAV 04000401 LAHRFRFESR TAGKQGSRFS LGGPCADPVR VRPRRSR

Alphafold2

297A1_m5.pdb

.fasta

Heme Docked

PDBj

CYP298

>CYP298A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSVPTAVGAA CPGEVFDPLS PSMLADPYLV YERLRTEAPV HWHEHLRAWV 00500051 LTRHEDCVGV LRRPEVFGSD PRKLAKPIPE SVISLQTMDP PEHSAVRHHF 01000101 LAKLRRQDLD AWSARVRRIA VELLSNAGPD PFDFVAEVAE PLALHSMCSL 01500151 CGVPYPEDDE RFRTASRTMV LGMDAGLEPA RREPALAARG VLNEIIEDWI 02000201 ARAPRESLIS GLDEVSGIER GYLVNSARAV FDAGYSTTGN MLGNIVKWLL 02500251 GPGGVRETAP LGGLDSRAVE ELGRIEGVVQ AVSRHCLAAV EIGGRTLRRG 03000301 DVVIVMLAAA NHDPKVFPDP RRADFSRESG PHLVFGRGVH SCLGGHVAGR 03500351 VALALLHALA DGFERIEPAG PHRQRPTATQ RGLDHLPIRL S

Alphafold2

298A1_m3.pdb

.fasta

Heme Docked

PDBj

CYP299

>CYP2A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSTRLPEPPE AGLLNALRFG TDTFRFLEGI QARFDDGTSV SIPGRPPLVV 00500051 LTGPDLVGEA LDRPEDFPRV PAQDAVTMIA ENGLVQSEGA LWSQQRSVMA 01000101 PSFSGEQVTA YANTTGRRIE ARADQWAAAG ARTTDLHREM TSLTVRVASE 01500151 ILLGEDIGTE RADQFHEWMQ VAGEEFEFGI ETVLPDWVPT PTSGEFEQAA 02000201 AGIRELSEQL IERRRESLAA GERPDASDML TMLIRAEDNP DIDYPENQIR 02500251 DEVATFLIAG HETTALSLTY TLCLLSWHPE ARRRVRQEAD EALGDGPPTH 03000301 DDLAELTYTR RVYDEALRLY PPAWGVFRQA NGDVTLGQYT IPDGSAVIMP 03500351 LWSIHRDGSY FEQPDTFDPD RWERRTPRAV DAYRPFSSGP HACIGRGFAL 04000401 AGSTLVLARL VRDFDIDVPE SALDDLRLTP TLRPADGVEA TIQPVGGR