Reference

Cytochrome P450 HomepageNelson, DR (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfold

Cytochrome P450 HomepageComprehensive Bacterial P450shttps://drnelson.uthsc.edu/comprehensive-bacterial-p450s/

D. R. Nelson's

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm,  recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output AlphaFold2_advanced.ipynb (for the preparation of movie)

CYP101

>CYP101A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTETIQSNA NLAPLPPHVP EHLVFDFDMY NPSNLSAGVQ EAWAVLQESN 00500051 VPDLVWTRCN GGHWIATRGQ LIREAYEDYR HFSSECPFIP REAGEAYDFI 01000101 PTSMDPPEQR QFRALANQVV GMPVVDKLEN RIQELACSLI ESLRPQGQCN 01500151 FTEDYAEPFP IRIFMLLAGL PEEDIPHLKY LTDQMTRPDG SMTFAEAKEA 02000201 LYDYLIPIIE QRRQKPGTDA ISIVANGQVN GRPITSDEAK RMCGLLLVGG 02500251 LDTVVNFLSF SMEFLAKSPE HRQELIERPE RIPAACEELL RRFSLVADGR 03000301 ILTSDYEFHG VQLKKGDQIL LPQMLSGLDE RENACPMHVD FSRQKVSHTT 03500351 FGHGSHLCLG QHLARREIIV TLKEWLTRIP DFSIAPGAQI QHKSGIVSGV 04000401 QALPLVWDPA TTKAV

MMSeqs2

Alphafold2

PDBj 1.3Å

CYP101A1 (P450cam) WT

Colabfold_advanced outputs

CYP102

>CYP102A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTIKEMPQPK TFGELKNLPL LNTDKPVQAL MKIADELGEI FKFEAPGRVT 00500051 RYLSSQRLIK EACDESRFDK NLSQALKFVR DFAGDGLFTS WTHEKNWKKA 01000101 HNILLPSFSQ QAMKGYHAMM VDIAVQLVQK WERLNADEHI EVPEDMTRLT 01500151 LDTIGLCGFN YRFNSFYRDQ PHPFITSMVR ALDEAMNKLQ RANPDDPAYD 02000201 ENKRQFQEDI KVMNDLVDKI IADRKASGEQ SDDLLTHMLN GKDPETGEPL 02500251 DDENIRYQII TFLIAGHETT SGLLSFALYF LVKNPHVLQK AAEEAARVLV 03000301 DPVPSYKQVK QLKYVGMVLN EALRLWPTAP AFSLYAKEDT VLGGEYPLEK 03500351 GDELMVLIPQ LHRDKTIWGD DVEEFRPERF ENPSAIPQHA FKPFGNGQRA 04000401 CIGQQFALHE ATLVLGMMLK HFDFEDHTNY ELDIKETLTL KPEGFVVKAK 04500451 SKKIPLGGIP --(Fdx)--(FdR)

MMSeqs2

Alphafold2

PDBj 1.2Å

CYP102A1 (P450BM3) WT

EMDB 6.7Å

Colabfold_advanced outputs

CYP103

>CYP103A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIANSSTDVS VADQKFLNVA KSNQIDPDAV PISRLDSEGH SIFAEWRPKR 00500051 PFLRREDGIF LVLRADHIFL LGTDPRTRQI ETELMLNRGV KAGAVFDFID 01000101 HSMLFSNGET HGKRRSGLSK AFSFRMVEAL RPEIAKITEC LWDDLQKVDD 01500151 FNFTEMYASQ LPALTIASVL GLPSEDTPFF TRLVYKVSRC LSPSWRDEEF 02000201 EEIEASAIEL QDYVRSVIAD SGRRMRDDFL SRYLKAVREA GTLSPIEEIM 02500251 QLMLIILAGS DTTRTAMVMV TALALQNPAL WSSLRGNQSY VAAAVEEGLR 03000301 FEPPVGSFPR LALKDIDLDG YVLPKGSLLA LSVMSGLRDE KHYEHPQLFD 03500351 VGRQQMRWHL GFGAGVHRCL GETLARIELQ EGLRTLLRRA PNLAVVGDWP 04000401 RMMGHGGIRR ATDMMVKLSF DL

Colabfold_advanced outputs

CYP104

>CYP104A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEERRVSISS ITWRFPMLFA PVDDVTTIDD LTLDPYPIYR RMRVQNPVVH 00500051 VASVRRTFLT KAFDTKMVKD DPSRFSSDDP STPMKPAFQA HTLMRKDGTE 01000101 HARERMAMAR AFAPKAIADH WAPIYRDIVN EYLDRLPRGD TVDLFAEICG 01500151 PVAARILAHI LGICEASDVE IIRWSQRLID GAGNFGWRSE LFERSDEANA 02000201 EMNCLFNDLV KKHRSAPNPS AFATMLNAPD PIPLSQIYAN IKIAIGGGVN 02500251 EPRDALGTIL YGLLTNPEQL EEVKRQQCWG QAFEEGLRWV APIQASSRLV 03000301 REDTEIRGFI VPKGDIVMTI QASANRDEDV FEDGESFNVF RPKSAHQSFG 03500351 SGPHHCPGAQ ISRQTVGAIM LPILFDRFPD MILPHPELVQ WRGFGFRGPI 04000401 NLPVTLR

Colabfold_advanced outputs

CYP105

>CYP105A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTDTATTPQT TDAPAFPSNR SCPYQLPDGY AQLRDTPGPL HRVTLYDGRQ 00500051 AWVVTKHEAA RKLLGDPRLS SNRTDDNFPA TSPRFEAVRE SPQAFIGLDP 01000101 PEHGTRRRMT ISEFTVKRIK GMRPEVEEVV HGFLDEMLAA GPTADLVSQF 01500151 ALPVPSMVIC RLLGVPYADH EFFQDASKRL VQSTDAQSAL TARNDLAGYL 02000201 DGLITQFQTE PGAGLVGALV ADQLANGEID REELISTAML LLIAGHETTA 02500251 SMTSLSVITL LDHPEQYAAL RADRSLVPGA VEELLRYLAI ADIAGGRVAT 03000301 ADIEVEGHLI RAGEGVIVVN SIANRDGTVY EDPDALDIHR SARHHLAFGF 03500351 GVHQCLGQNL ARLELEVILN ALMDRVPTLR LAVPVEQLVL RPGTTIQGVN 04000401 ELPVTW

Alphafold2

PDBj 1.5Å

CYP105A1 WT
(H308Q from Nelson's)

Colabfold_advanced outputs

CYP106

>CYP106A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNKEVIPVTE IPKFQSRAEE FFPIQWYKEM LNNSPVYFHE ETNTWNVFQY 00500051 EHVKQVLSNY DFFSSDGQRT TIFVGDNSKK KSTSPITNLT NLDPPDHRKA 01000101 RSLLAAAFTP RSLKNWEPRI KQIAADLVEA IQKNSTINIV DDLSSPFPSL 01500151 VIADLFGVPV KDRYQFKKWV DILFQPYDQE RLEEIEQEKQ RAGAEYFQYL 02000201 YPIVIEKRSN LSDDIISDLI QAEVDGETFT DEEIVHATML LLGAGVETTS 02500251 HAIANMFYSF LYDDKSLYSE LRNNRELAPK AVEEMLRYRF HISRRDRTVK 03000301 QDNELLGVKL KKGDVVIAWM SACNMDETMF ENPFSVDIHR PTNKKHLTFG 03500351 NGPHFCLGAP LARLEMKIIL EAFLEAFSHI EPFEDFELEP HLTASATGQS 04000401 LTYLPMTVYR 

Alphafold2

PDBj 1.8Å

CYP106A2 (P450MEG) WT

Colabfold_advanced outputs

CYP107

>CYP107A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTVPDLESD SFHVDWYRTY AELRETAPVT PVRFLGQDAW LVTGYDEAKA 00500051 ALSDLRLSSD PKKKYPGVEV EFPAYLGFPE DVRNYFATNM GTSDPPTHTR 01000101 LRKLVSQEFT VRRVEAMRPR VEQITAELLD EVGDSGVVDI VDRFAHPLPI 01500151 KVICELLGVD EKYRGEFGRW SSEILVMDPE RAEQRGQAAR EVVNFILDLV 02000201 ERRRTEPGDD LLSALIRVQD DDDGRLSADE LTSIALVLLL AGFEASVSLI 02500251 GIGTYLLLTH PDQDQLALVR RDPSALPNAV EEILRYIAPP ETTTRFAAEE 03000301 VEIRGVAIPQ YSTVLVANGA ANRDPKQFPD PHRFDVTRDT RGHLSFGQGI 03500351 HFCMGRPLAK LEGEVALRAL FGRFPALSLG IDADDVVWRR SLLLRGIDHL 04000401 PVRLDG

Alphafold2

PDBj 1.7Å

CYP107A1 (P450eryF) WT(Δ264-265 from Nelson's)

Colabfold_advanced outputs

CYP108

>CYP108A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDARATIPEH IARTVILPQG YADDEVIYPA FKWLRDEQPL AMAHIEGYDP 00500051 MWIATKHADV MQIGKQPGLF SNAEGSEILY DQNNEAFMRS ISGGCPHVID 01000101 SLTSMDPPTH TAYRGLTLNW FQPASIRKLE ENIRRIAQAS VQRLLDFDGE 01500151 CDFMTDCALY YPLHVVMTAL GVPEDDEPLM LKLTQDFFGV HEPDEQAVAA 02000201 PRQSADEAAR RFHETIATFY DYFNGFTVDR RSCPKDDVMS LLANSKLDGN 02500251 YIDDKYINAY YVAIATAGHD TTSSSSGGAI IGLSRNPEQL ALAKSDPALI 03000301 PRLVDEAVRW TAPVKSFMRT ALADTEVRGQ NIKRGDRIML SYPSANRDEE 03500351 VFSNPDEFDI TRFPNRHLGF GWGAHMCLGQ HLAKLEMKIF FEELLPKLKS 04000401 VELSGPPRLV ATNFVGGPKN VPIRFTKA

Alphafold2

PDBj 2.3Å

CYP108A1 (P450Terp) WT

Colabfold_advanced outputs

CYP109

>CYP109A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTNQTARSSK KERYANLIPM EELHSEKDRL FPFPIYDKLR RESPVRYDPL 00500051 RDCWDVFKYD DVQFVLKNPK LFSSKRGIQT ESILTMDPPK HTKLRALVSR 01000101 AFTPKAVKQL ETRIKDVTAF LLQEARQKST IDIIEDFAGP LPVIIIAEML 01500151 GAPIEDRHLI KTYSDVLVAG AKDSSDKAVA DMVHNRRDGH AFLSDYFRDI 02000201 LSKRRAEPKE DLMTMLLQAE IDGEYLTEEQ LIGFCILLLV AGNETTTNLI 02500251 ANAVRYLTED SVVQQQVRQN TDNVANVIEE TLRYYSPVQA IGRVATEDTE 03000301 LGGVFIKKGS SVISWIASAN RDEDKFCKPD CFKIDRPSYP HLSFGFGIHF 03500351 CLGAPLARLE ANIALSSLLS MSACIEKAAH DEKLEAIPSP FVFGVKRLPV 04000401 RITFK

Alphafold2

PDBj 2.7Å

CYP109A2 (P450BM1) WT

PDBj 1.8Å

CYP109B1 WT

Colabfold_advanced outputs

CYP110

>CYP110A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLTQLPNPIS VPSWWQLINW IADPIGFQKK YSKKYGNIFS MQLAGIGSFV 00500051 ILGEPQALQE IFTQDSRFDV GRGNTLAEPL IGRTSLMLMD GDRHRRERKL 01000101 LMPPFHGERL QAYAQQICLI TNQIASEWQI GQPFVARSAM QKLSLEVIIQ 01500151 IVFGLADGER YQQIKPLFTD WLNMTDSPLR SSMLFLKSLQ KDWGTWTPWG 02000201 QMKHKQRSIY DLLQAEIEEK RTKENEQRGD VLSLMMAARD ENGQAMTDEE 02500251 LKDELLTILF AGHETTATTI AWAFYQILKN VNVQEKLQQE LDRLGANPNP 03000301 MEIAQLPYLT AVSQETLRMY PVLPTLFPRI TKSSINIAGY QLEPDTTLMA 03500351 SIYLIHYRED LYPNPQQFRP ERFIERQYSP SEYIPFGGGS RRCLGIALAL 04000401 LEIKLVIATV LSNYQLALAE DKPVNVQRRG FTLAPDGGVR VIMTGKKSLK 04500451 FEQSSKIFN

Colabfold_advanced outputs

CYP111

>CYP111A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MERPDLKNPD LYTQQVPHDI FARLRREEPV YWNPESDGSG FWAVLRHKDI 00500051 IEVSRQPLLF SSAYENGGHR IFNENEVGLT NAGEAAVGVP FISLDPPVHT 01000101 QYRKVIMPAL SPARLGDIEQ RIRVRAEALI ERIPLGEEVD LVPLLSAPLP 01500151 LLTLAELLGL DPDCWYELYN WTNAFVGEDD PEFRKSPEDM AKVLGEFMGF 02000201 CQELFESRRA NPGPDIATLL ANAEINGQPV ALRDFIGNLT LTLVGGNETT 02500251 RNSISHTIVT LSQQPDQWDI LRQRPELLKT ATAEMVRHAS PVLHMRRTAM 03000301 EDTEIGGQAI AKGDKVVLWY ASGNRDESVF SDADRFDVTR TGVQHVGFGS 03500351 GQHVCVGSRL AEMQLRVVFE ILSTRVKRFE LCSKSRRFRS NFLNGLKNLN 04000401 VVLVPK

Colabfold_advanced outputs

CYP112

>CYP112A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSEQQPLPTL PMWRVDHIEP SPEMLALRAN GPIHRVRFPS GHEGWWVTGY 00500051 DEAKAVLSDA AFRPAGMPPA AFTPDSVILG SPGWLVSHEG REHARLRAIV 01000101 APAFSDRRVK LLVQQVEAIA AHLFETLAAQ PQPADLRRHL SFPLPAMVIS 01500151 ALMGVLYEDH AFFAGLSDEV MTHQHESGPR SASRLAWEEL RAYIRGKMRD 02000201 KRQDPDDNLL TDLLAAVDQG KASEEEAVGL AAGMLVAGHE STVAQIEFGL 02500251 HAMFRHPQQR ERLVGDPSLV DKAVQEILRM YPPGAGWDGI MRYPRTDVTI 03000301 AGEHIPAESK VLVGLPATSF DPHHFDDPEI FDIERQEKPH LAFSYGPHAC 03500351 IGVALARLEL KVVFGSIFQR LPALRLAVAP EQLKLRKEII TGGFEQFPVL 04000401 W
>CYP112A1 [Bradyrhizobium diazoefficiens USDA 110] AAC28889.1

Colabfold_advanced outputs

CYP113

>CYP113A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTIDEVPGM ADETALLDWL GTMREKQPVW QDRYGVWHVF RHADVQTVLR 00500051 DTATFSSDPT RVIEGASPTP GMIHEIDPPE HRALRKVVSS AFTPRTISDL 01000101 EPRIRDVTRS LLADAGESFD LVDVLAFPLP VTIVAELLGL PPMDHEQFGD 01500151 WSGALVDIQM DDPTDPALAE RIADVLNPLT AYLKARCAER RADPGDDLIS 02000201 RLVLAEVDGR ALDDEEAANF STALLLAGHI TTTVLLGNIV RTLDEHPAHW 02500251 DAAAEDPGRI PAIVEEVLRY RPPFPQMQRT TTKATEVAGV PIPADVMVNT 03000301 WVLSANRDSD AHDDPDRFDP SRKSGGAAQF SFGHGVHFCL GAPLARLENR 03500351 VALEEIIARF GRLTVDRDDE RLRHFEQIVL GTRHLPVLAG SSPRQSA
>CYP113A1 [Saccharopolyspora erythraea NRRL 2338] AAC45584.1

Alphafold2

PDBj 1.6Å

CYP113A1 (P450EryK) WT　(F330L from Nelson's)

Colabfold_advanced outputs

CYP114

>CYP114A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLSRHADIFW AFKATGDAFR GPAPGELARY FSRAATSPSL NLLASTLAMK 00500051 DPPTHTRLRR LISRDFTMGQ IDNLRPSIAR IVAARLDGIT PALERGEAVD 01000101 LHREFALALP MLVFAELFGM PQDDMFELAA GIGTILEGLG PHASDPQLAA 01500151 ADAASARVQA YFGDLIQRKR TDPRRDIVSM LVGAHDDDAD TLSDAELISM 02000201 LWGMLLGGFV TTAASIDHAV LAMLAYPEQR HWLQADAARV RAFVEEVLRC 02500251 DAPAMFSSIP RIAQRDIELG GVVIPKNADV RVLIASGNRD PDAFADPDRF 03000301 DPARFYGTSP GMSTDGKIML SFGHGIHFCL GAQLARVQLA ESLPRIQARF 03500351 PTLAFAGQPT REPSAFLRTF RTLPVRLHAQ GS
>CYP114A1 [Bradyrhizobium diazoefficiens USDA 110] AAC28890.1

Colabfold_advanced outputs

CYP115 

>CYP115A2v1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPAAPTQLDR LSSAILRQGG MARVSLPGDV VTWAAARHQT LRQMLSDQRF 00500051 NKDWRQWRAL QDGEIPEDHP LIGICKVDNM TTAHGADHRR LRGLLSSSFA 01000101 PSRIALLAPR VEQCVDRLLA EMAQRGGSAD LMSEFAAPLP TNVIAELFGL 01500151 PDEQREEIVA LTYSLASTSA TAEEVRQTRQ RIPEFFRRLI ALKRGQLGDD 02000201 LASALIVARD KGELVSDTEL IDMLFMVLSA GFVTTAGVIG NGVLALLTHP 02500251 QQLHLVRSGQ VPWSQAIEEI LRWGTSAANL PFRYATQDVE IDGCLVRRGD 03000301 AVLMAFHAAN RDEKAFGPGA NRFDVTRRHN PHLSFGEGPH SCLGAALARL 03500351 ELRCAFPPLF GRLEDLALTI AAEDVVYMPS YVIRCPQRLP VSFRPSVA
>CYP115A1 [Mesorhizobium japonicum MAFF 303099] BAB52466.1

Alphafold2

41% to CYP154A1

39% to CYP154C1 


Highly conserved in Mesorhizobium and Xanthomonas

Colabfold_advanced outputs

CYP116

>CYP116A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVDHAPEGV KSPTGCPVSG MAADFDPFRG AYQVDPSSSL RQARKDEPVF 00500051 FSPLLDYWVV TRYEDIKQIF KTPSVFSPSI TVDQITPISD EALQILGSYQ 01000101 FAAGRMLVNE DEPIHTERRR LLMQPFEADN VATLEPKIRE VVNTYLDRVI 01500151 KDGRADLIGD LLYEVPCIVA LIFLGVPDED IETCRQYGMQ QTLFTWGHPT 02000201 GDEQTRVATG MGKFWEFAGG LVDKLKADPN AKGWIPHAIE MQRQHPDLFD 02500251 DNYLQNIMFG GVFAAHETTT NATGNAFRTL LENRSSWDEI CADPTLIPKA 03000301 IEECLRYSGS VVAWRRKAVV DTTVGEVDIP AGGRLLIVMA SANRDDSMFP 03500351 EPDDFDIHRG NAQRHLTFGI GSHTCLGATL ARLEMKVFLE EVSRRLPHMS 04000401 LVAGQEFSYL PNTSFRGPEH VLVEWDPQQN PVPADRP
>CYP116A1 [Rhodococcus erythropolis] WP_222671509.1

Alphafold2

PDBj 1.4Å

CYP116B46 WT (20-445)

PDBj 2.1Å

CYP116B46 WT full

Colabfold_advanced outputs

CYP117

>CYP117A1_Nterminal is trancated---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MFGGTLVAQD GIAHRQARDA IQAALLPKGL TLAGIGELFA PVIRARVQRW 00500051 RERGDVTILR ETGDLMLKLI FSLMGIPAQD LPGWHRKYRQ LLQLIVAPPV 01000101 DLPGLPLRRG RAARDWIDAR LREFVRAARE HASRTGLIND MVSAFDRSDD 01500151 ALSDDVLVAN IRLLLLGGHD TTASTMAWMV IELARQPGLW DALVEEAQRV 02000201 GAVPTRHADL AQCPVAEALF RETLRVHPAT PLLVRRALRE LRIGQQRIPT 02500251 GTDLCIPLLH FSTSALLHEA PDQFRLARWL QRTEPIRPVD MLQFGTGPHF 03000301 CMGYHLVWLE LVQFCIALAL TMHEAGVRPR LLSGVEKGRR YYPTAHPSMT 03500351 IRIGFS
>CYP117A1 [Bradyrhizobium diazoefficiens USDA 110] AAC28893.1

CYP117

>CYP117A1_+Naa90---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLLNPLNRRH RLRNDIPVVP GAFPLVGHLP AVVCDLPRLL RRAERTLGSH 00500051 FWLDFGPAGH LMTSLDPDAL ALLRHKDVSS GLIEDIAPEL FGGTLVAQDG 01000101 IAHRQARDAI QAALLPKGLT LAGIGELFAP VIRARVQRWR ERGDVTILRE 01500151 TGDLMLKLIF SLMGIPAQDL PGWHRKYRQL LQLIVAPPVD LPGLPLRRGR 02000201 AARDWIDARL REFVRAAREH ASRTGLINDM VSAFDRSDDA LSDDVLVANI 02500251 RLLLLGGHDT TASTMAWMVI ELARQPGLWD ALVEEAQRVG AVPTRHADLA 03000301 QCPVAEALFR ETLRVHPATP LLVRRALREL RIGQQRIPTG TDLCIPLLHF 03500351 STSALLHEAP DQFRLARWLQ RTEPIRPVDM LQFGTGPHFC MGYHLVWLEL 04000401 VQFCIALALT MHEAGVRPRL LSGVEKGRRY YPTAHPSMTI RIGFS
>CYP117A1 [Bradyrhizobium diazoefficiens USDA 110] BCE72108.1

Colabfold_advanced outputs

Colabfold_advanced outputs

CYP118

PSEUDOGENE

CYP119

>CYP119A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MYDWFSEMRK KDPVYYDGNI WQVFSYRYTK EVLNNFSKFS SDLTGYHERL 00500051 EDLRNGKIRF DIPTRYTMLT SDPPLHDELR SMSADIFSPQ KLQTLETFIR 01000101 ETTRSLLDSI DPREDDIVKK LAVPLPIIVI SKILGLPIED KEKFKEWSDL 01500151 VAFRLGKPGE IFELGKKYLE LIGYVKDHLN SGTEVVSRVV NSNLSDIEKL 02000201 GYIILLLIAG NETTTNLISN SVIDFTRFNL WQRIREENLY LKAIEEALRY 02500251 SPPVMRTVRK TKERVKLGDQ TIEEGEYVRV WIASANRDEE VFHDGEKFIP 03000301 DRNPNPHLSF GSGIHLCLGA PLARLEARIA IEEFSKRFRH IEILDTEKVP 03500351 NEVLNGYKRL VVRLKSNE
>CYP119A1 [Sulfolobus acidocaldarius] >WP_011278877.1

Colabfold_advanced outputs

CYP120

>CYP120A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MITSPTNLNS LPIPPGDFGL PWLGETLNFL NDGDFGKKRQ QQFGPIFKTR 00500051 LFGKNVIFIS GALANRFLFT KEQETFQATW PLSTRILLGP NALATQMGEI 01000101 HRSRRKILYQ AFLPRTLDSY LPKMDGIVQG YLEQWGKANE VIWYPQLRRM 01500151 TFDVAATLFM GEKVSQNPQL FPWFETYIQG LFSLPIPLPN TLFGKSQRAR 02000201 ALLLAELEKI IKARQQQPPS EEDALGILLA ARDDNNQPLS LPELKDQILL 02500251 LLFAGHETLT SALSSFCLLL GQHSDIRERV RQEQNKLQLS QELTAETLKK 03000301 MPYLDQVLQE VLRLIPPVGG GFRELIQDCQ FQGFHFPKGW LVSYQISQTH 03500351 ADPDLYPDPE KFDPERFTPD GSATHNPPFA HVPFGGGLRE CLGKEFARLE 04000401 MKLFATRLIQ QFDWTLLPGQ NLELVVTPSP RPKDNLRVKL HSLM
>CYP120A1 [Synechocystis sp. PCC 6803] AGF52788.1

Colabfold_advanced outputs

CYP121

>CYP121A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTATVLLEVP FSARGDRIPD AVAELRTREP IRKVRTITGA EAWLVSSYAL 00500051 CTQVLEDRRF SMKETAAAGA PRLNALTVPP EVVNNMGNIA DAGLRKAVMK 01000101 AITPKAPGLE QFLRDTANSL LDNLITEGAP ADLRNDFADP LATALHCKVL 01500151 GIPQEDGPKL FRSLSIAFMS SADPIPAAKI NWDRDIEYMA GILENPNITT 02000201 GLMGELSRLR KDPAYSHVSD ELFATIGVTF FGAGVISTGS FLTTALISLI 02500251 QRPQLRNLLH EKPELIPAGV EELLRINLSF ADGLPRLATA DIQVGDVLVR 03000301 KGELVLVLLE GANFDPEHFP NPGSIELDRP NPTSHLAFGR GQHFCPGSAL 03500351 GRRHAQIGIE ALLKKMPGVD LAVPIDQLVW RTRFQRRIPE RLPVLW
>CYP121A1 [Mycobacterium tuberculosis] WP_003411685.1

CYP122

>CYP122A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSTDTPAGTP QAGRCPFAIQ DAHRAILDSA TVGSFDLFGI KHWLVAAAED 00500051 VKLVTNDPRF SSAAPSEMLP DRRPGWFSGM DVPEHSRYRQ KIAGDFTLRA 01000101 ARRQEEFVFE AADACLDEIE AAGPGADLVP GYAKRLPSLV INALYGLTPE 01500151 EGAVLETRMR GITGSTDLDS VKTLTDDFFA HALELVRAKR DERGDDLLHR 02000201 LASAEDGEIP LSDDEATGVF ATLLFAGHDS VQQMVGYCLY ALLSHPEQEG 02500251 ALRTRPELID GAVEEMLRFL PVNQMGVPRV CVADVDLRGV RISVGDNVIP 03000301 LYSTANRDPE VFPDPDTFDV SRPTEGNFAF GHGVHKCPGQ HVARLLIKVA 03500351 CLRLLERFPD VRLVGDVPMN EGLGLFSPAE LRITWGAA
>CYP122A1 [Streptomyces tsukubensis] WP_077973941

CYP123

>CYP123A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVRVGDPEL VLDPYDYDFH EDPYPYYRRL RDEAPLYRNE ERNFWAVSRH 00500051 HDVLQGFRDS TALSNAYGVS LDPSSRTSEA YRVMSMLAMD DPAHLRMRTL 01000101 VSKGFTPRRI RELEPQVLEL ARIHLDSALQ TESFDFVAEF AGKLPMDVIS 01500151 ELIGVPDTDR ARIRALADAV LHREDGVADV PPPAMAASIE LMRYYADLIA 02000201 EFRRRPANNL TSALLAAELD GDRLSDQEIM AFLFLMVIAG NETTTKLLAN 02500251 AVYWAAHHPG QLARVFADHS RIPMWVEETL RYDTSSQILA RTVAHDLTLY 03000301 DTTIPEGEVL LLLPGSANRD DRVFDDPDDY RIGREIGCKL VSFGSGAHFC 03500351 LGAHLARMEA RVALGALLRR IRNYEVDDDN VVRVHSSNVR GFAHLPISVQ 04000401 AR
>CYP123A1 [Mycobacterium tuberculosis] >WP_003403911.1

CYP124 <<1aa insertion>>

>CYP124A1 C_1  1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGIAPRVNGA APPDVPLSEI NLGSWDFWAL DDDIRDGAFA TLRREAPISF 00500051 HPAFDTEEGF PQGAGHWALT RHDDVFYASR HPELFSSASG IVIGDQTPEL 01000101 AEYFGSMIAM DDPRHTRLRN IVRSAFTPRV LAVIEDSVRD RARRLVAGMV 01500151 DRNPDGHAEL VTELAGPLPL QIICDMMGIP ESDHQQIFHW TNVILGFGDP 02000201 DLTTDFDEFV TVAMDIGAYA TALADQRRSA PADDLTTSLV QAEVDGERLT 02500251 SAEVASFFIL LVVAGNETTR NAISHGVLAL TRYPEQRRLW WSRFDELAPT 03000301 AVEEIVRWAS PVSYMRRTVT RDTELAGTAL PAGSKVTLWY GSANRDETKF 03500351 DNPWMFDVQR HPNPHVGFGG GGAHFCLGAN LARREITVLF SELHRHLPDI 04000401 VATEEPDRLQ SAFIHGIKRL PVAWR
>CYP124A1 [Mycolicibacterium smegmatis MC2 155] >AFP39933.1

CYP125 <<1aa insertion>>

>CYP125A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSWNHQSVEI AVRRTTVPSP NLPPGFDFTD PAIYAERLPV AEFAELRSAA 00500051 PIWWNGQDPG KGGGFHDGGF WAITKLNDVK EISRHSDVFS SYENGVIPRF 01000101 KNDIAREDIE VQRFVMLNMD APHHTRLRKI ISRGFTPRAV GRLHDELQER 01500151 AQKIAAEAAA AGSGDFVEQV SCELPLQAIA GLLGVPQEDR GKLFHWSNEM 02000201 TGNEDPEYAH IDPKASSAEL IGYAMKMAEE KAKNPADDIV TQLIQADIDG 02500251 EKLSDDEFGF FVVMLAVAGN ETTRNSITQG MMAFAEHPDQ WELYKKVRPE 03000301 TAADEIVRWA TPVTAFQRTA LRDYELSGVQ IKKGQRVVMF YRSANFDEEV 03500351 FQDPFTFNIL RNPNPHVGFG GTGAHYCIGA NLARMTINLI FNAVADHMPD 04000401 LKPISAPERL RSGWLNGIKH WQVDYTGRCP VAH
>CYP125A1 [Mycobacterium tuberculosis] >WP_003419304.1

Alphafold2

PDBj 1.6Å

CYP125A1 WT
42% CYP12440.4% CYP268A2

CYP126

>CYP126A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTAAGLSGI DLTDLDNFAD GFPHHLFAIH RREAPVYWHR PTEHTPDGEG 00500051 FWSVATYAET LEVLRDPVTY SSVTGGQRRF GGTVLQDLPV AGQVLNMMDD 01000101 PRHTRIRRLV SSGLTPRMIR RVEDDLRRRA RGLLDGVEPG APFDFVVEIA 01500151 AELPMQMICI LLGVPETDRH WLFEAVEPGF DFRGSRRATM PRLNVEDAGS 02000201 RLYTYALELI AGKRAEPADD MLSVVANATI DDPDAPALSD AELYLFFHLL 02500251 FSAGAETTRN SIAGGLLALA ENPDQLQTLR SDFELLPTAI EEIVRWTSPS 03000301 PSKRRTASRA VSLGGQPIEA GQKVVVWEGS ANRDPSVFDR ADEFDITRKP 03500351 NPHLGFGQGV HYCLGANLAR LELRVLFEEL LSRFGSVRVV EPAEWTRSNR 04000401 HTGIRHLVVE LRGG
>CYP126A1 [Mycobacterium tuberculosis CCDC5079] >AEJ45910.1

Heme Docked

CYP127

>CYP127A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSDLRRKRVK TNPIPDHVPP ALVRHFSLFT SPGMAPTPNG DPHAAVACVH 00500051 DDGPPIFYSP SNTRDGRGTW VITRARDQRR VLEDTETFSS HRSIFASALG 01000101 EHWPVIPLEL DPPAHGVFRA LLNPLFSSRR VLALEPTIHA RAGALIDCIA 01500151 KEKTSCDVMK DFALPFTFSV FLSFLGLSQR RSEVLVGWVS DLLHGNAEKR 02000201 RAAARSVVAF IDEMAAMRRK SPAVDFMTFV VQAKIEGRSL TEEEVRGIGV 02500251 LFLVAGLDTV AAAIGFDMAY LARNPKHQEL LRNEPARLGL AAEELLRAYS 03000301 TVQIIRVATK DIEFEGVPIR EGDYVSCPAM IANRDPSEFK CPNTIDLARQ 03500351 DNQHTAFGYG PHLCHGAHLA RREIVIGLRE WLARIPAFRI KEGTAPITHG 04000401 GHVFGISNII LTWA
>CYP127A1 AE000101 Rhizobium sp. NGR234 same seq as Z68203 >Q53215.1

Heme Docked

PDBj

CYP128

>CYP128A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTATQSPPEP APDRVRLAGC PLAGTPDVGL TAQDATTALG VPTRRRASSG 00500051 GIPVATSMWR DAQTVRTYGP AVAKALALRV AGKARSRLTG RHCRKFMQLT 01000101 DFDPFDPAIA ADPYPHYREL LAGERVQYNP KRDVYILSRY ADVREAARNH 01500151 DTLSSARGVT FSRGWLPFLP TSDPPAHTRM RKQLAPGMAR GALETWRPMV 02000201 DQLARELVGG LLTQTPADVV STVAAPMPMR AITSVLGVDG PDEAAFCRLS 02500251 NQAVRITDVA LSASGLISLV QGFAGFRRLR ALFTHRRDNG LLRECTVLGK 03000301 LATHAEQGRL SDDELFFFAV LLLVAGYEST AHMISTLFLT LADYPDQLTL 03500351 LAQQPDLIPS AIEEHLRFIS PIQNICRTTR VDYSVGQAVI PAGSLVLLAW 04000401 GAANRDPRQY EDPDVFRADR NPVGHLAFGS GIHLCPGTQL ARMEGQAILR 04500451 EIVANIDRIE VVEPPTWTTN ANLRGLTRLR VAVTPRVAP
>CYP128 Rv2268c Z77163 Mycobacterium tuberculosis >P63714.1

Heme Docked

PDBj

CYP129

>CYP129A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSGEAPRVAV DPFSCPMMTM QRKPEVHDAF REAGPVVEVN APAGGPAWVI 00500051 TDDALAREVL ADPRFVKDPD LAPTAWRGVD DGLDIPVPEL RPFTLIAVDG 01000101 EDHRRLRRIH APAFNPRRLA ERTDRIAAIA DRLLTELADS SDRSGEPAEL 01500151 IGGFAYHFPL LVICELLGVP VTDPAMAREA VGVLKALGLG GPQSAGGDGT 02000201 DPAGDVPDTS ALESLLLEAV HAARRKDTRT MTRVLYERAQ AEFGSVSDDQ 02500251 LVYMITGLIF AGHDTTGSFL GFLLAEVLAG RLAADADGDA ISRFVEEALR 03000301 HHPPVPYTLW RFAATEVVIR GVRLPRGAPV LVDIEGTNTD GRHHDAPHAF 03500351 HPDRPSRRRL TFGDGPHYCI GEQLAQLESR TMIGVLRSRF PQARLAVPYE 04000401 ELRWCRKGAQ TARLTDLPVW LR

Heme Docked

PDBj

CYP130

>CYP130A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSVMSHEFQ LATAETWPNP WPMYRALRDH DPVHHVVPPQ RPEYDYYVLS 00500051 RHADVWSAAR DHQTFSSAQG LTVNYGELEM IGLHDTPPMV MQDPPVHTEF 01000101 RKLVSRGFTP RQVETVEPTV RKFVVERLEK LRANGGGDIV TELFKPLPSM 01500151 VVAHYLGVPE EDWTQFDGWT QAIVAANAVD GATTGALDAV GSMMAYFTGL 02000201 IERRRTEPAD DAISHLVAAG VGADGDTAGT LSILAFTFTM VTGGNDTVTG 02500251 MLGGSMPLLH RRPDQRRLLL DDPEGIPDAV EELLRLTSPV QGLARTTTRD 03000301 VTIGDTTIPA GRRVLLLYGS ANRDERQYGP DAAELDVTRC PRNILTFSHG 03500351 AHHCLGAAAA RMQCRVALTE LLARCPDFEV AESRIVWSGG SYVRRPLSVP 04000401 FRVTS

Heme Docked

PDBj

CYP130A1 WT

CYP131 Proximal Cys was not exist

P450-like glycosyltransferase activator 

>CYP131A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPTPTSAPPA APTDSELGRH LLTVRGFHFV FGALGDPYAR RLRGEADHLS 00500051 LGELVRDRGP LHGSALGTWV TADGGISARL LDDPLLGPRH PASEGPQEHV 01000101 LENVWETWRT CHVTPLGEDL LTPAAADSDR LAALLGPVLG PRTCTAWQVD 01500151 AGRAVHRVLD GLPPHFDVVS DLARPAIAGS LAAVLGLPDE ARAELPDLLA 02000201 ACGPVLDSAL CPPRLPVARA MTQALRRVRE LMAAAVANHL TAPADGAVSA 02500251 LLAVDPGGGR DPGDTVTAAV LSTVVGAETA ITTVANAVMA LLKHDEQWSL 03000301 LRADPGRAAD AVEETLRWAP PVTLRSLITQ GEVQIGGETL EADQHVVVLV 03500351 DAAQRDPALY EDPDRFRLDR PRSPGFTHMA LAGRDHLGLV APLVRVQCTA 04000401 VLRALAERLP GLRAEGEPLR RGRSPVVRAP LSLRLAQK

gene dnrQ  duanosamine biosynthesis

possible sequence errors at C-terminal (no recognizable signature sequence in the last 68 amino acids)


PDBj

CYP132

>CYP132A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MATATTQRPL KGPAKRMSTW TMTREAITIG FDAGDGFLGR LRGSDITRFR 00500051 CAGRRFVSIS HPDYVDHVLH EARLKYVKSD EYGPIRATAG LNLLTDEGDS 01000101 WARHRGALNS TFARRHLRGL VGLMIDPIAD VTAARVPGAQ FDMHQSMVET 01500151 TLRVVANALF SQDFGPLVQS MHDLATRGLR RAEKLERLGL WGLMPRTVYD 02000201 TLIWCIYSGV HLPPPLREMQ EITLTLDRAI NSVIDRRLAE PTNSADLLNV 02500251 LLSADGGIWP RQRVRDEALT FMLAGHETTA NAMSWFWYLM ALNPQARDHM 03000301 LTELDDVLGM RRPTADDLGK LAWTTACLQE SQRYFSSVWI IAREAVDDDI 03500351 IDGHRIRRGT TVVIPIHHIH HDPRWWPDPD RFDPGRFLRC PTDRPRCAYL 04000401 PFGGGRRICI GQSFALMEMV LMAAIMSQHF TFDLAPGYHV ELEATLTLRP 04500451 KHGVHVIGRR R

Heme Docked

PDBj

CYP133

>CYP133B1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKLTDLSNPA FLENPYPLYE TLRAQAPFVS IGPNALMTGR YSLVDSLLHN 00500051 RNMGKKYMES MRVRYGDSAA DMPLFQAFSR MFITINPPAH THLRGLVMQA 01000101 FTGRESESMR PLAIDTAHQL IDNFEQKPSV DLVAEFAFPF PMQIICKMMD 01500151 VDIGDAVTLG IAVSKIAKVF DPSPMSADEL VHASTAYEEL AQYFTKLIEL 02000201 RRTHPGTDLI SMFLRAEEDG EKLTHDEIVS NVIMLLIAGY ETTSNMIGNA 02500251 LIALHRHPEQ LALLKSDLSL MPQAVSECLR YDGSVQFTMR AAMDDIEVEG 03000301 ELVPRGTVVF LMLGAANRDP AQFTHPDQLD ITRKQGRLQS FGAGIHHCLG 03500351 YRLALIELEC ALTTLFERLP HLRLAHLDAL NWNQRSNLRG VNTLIVDLHA 04000401 KN

Heme Docked

PDBj

CYP134

>CYP134A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSQSIKLFSV LSDQFQNNPY AYFSQLREED PVHYEESIDS YFISRYHDVR 00500051 YILQHPDIFT TKSLVERAEP VMRGPVLAQM HGKEHSAKRR IVVRSFIGDA 01000101 LDHLSPLIKQ NAENLLAPYL ERGKSDLVND FGKTFAVCVT MDMLGLDKRD 01500151 HEKISEWHSG VADFITSISQ SPEARAHSLW CSEQLSQYLM PVIKERRVNP 02000201 GSDLISILCT SEYEGMALSD KDILALILNV LLAATEPADK TLALMIYHLL 02500251 NNPEQMNDVL ADRSLVPRAI AETLRYKPPV QLIPRQLSQD TVVGGMEIKK 03000301 DTIVFCMIGA ANRDPEAFEQ PDVFNIHRED LGIKSAFSGA ARHLAFGSGI 03500351 HNCVGAAFAK NEIEIVANIV LDKMRNIRLE EDFCYAESGL YTRGPVSLLV 04000401 AFDGA

Heme Docked

PDBj 2.7Å

CYP134A1 WT(A356T from Nelson's)

PDBj 1.8Å

CYP134s (~49% to A1)
from Staphylococcus aureus

CYP135

>CYP135A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MASTLTTGLP PGPRLPRYLQ SVLYLRFREW FLPAMHRKYG DVFSLRVPPY 00500051 ADNLVVYTRP EHIKEIFAAD PRSLHAGEGN HILGFVMGEH SVLMTDEAEH 01000101 ARMRSLLMPA FTRAALRGYR DMIASVAREH ITRWRPHATI NSLDHMNALT 01500151 LDIILRVVFG VTDPKVKAEL TSRLQQIINI HPAILAGVPY PSLKRMNPWK 02000201 RFFHNQTKID EILYREIASR RIDSDLTART DVLSRLLQTK DTPTKPLTDA 02500251 ELRDQLITLL LAGHETTAAA LSWTLWELAH APEIQSQVVW AAVGGDDGFL 03000301 EAVLKEGMRR HTVIASTARK VTAPAEIGGW RLPAGTVVNT SILLAHASEV 03500351 SHPKPTEFRP SRFLDGSVAP NTWLPFGGGV RRCLGFGFAL TEGAVILQEI 04000401 FRRFTITAAG PSKGETPLVR NITTVPKHGA HLRLIPQRRL GGLGDSDPP

Heme Docked

PDBj

CYP136

>CYP136A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MPSISTTDYV LDQAKRRLKP TPVTIPGMGA VEKRLKDKHW DQFVLAEPPA 00500051 GSDLKPILGD AGLPIIGHMI EIFRGGPDFI LEIYRKHGPV YFAESPALSS 01000101 VMALGPDATQ AVFSNRNKDF SQRAWDPVIG PFFEGGLMLL DFDEHMFHRR 01500151 IMQEAFTRSR LTGYVSHIDS VASAVLANDW VANDPRFLFH PAIKELTLDI 02000201 ASEVFMGVPA GTDRALVTTV NQAFTTTTRA GNAIVRKPVP PLTWWRGIKA 02500251 RKTLEDYFSS RIGEKRRSES TDMFSVLCHS ADEDGQSFTD DQIVSHMIFL 03000301 MMAAHDTSTS TMTTMAYHLA ANPEWQDRLR EESARIGDGP LDIEALEKLE 03500351 TYDLVINEAL RMMTPLPFNF RQAIRDTDLL GYYIPAGTNV VTWPSINHRL 04000401 PELWTDPEKF DPERFAEPRS EHKKHRYAFA PFGGGAHKCI GMVFGQLEIK 04500451 TVVHRLLQNY RLELPRPDYQ PRYDYGGMPV PLDGMPIVLR PLH

Heme Docked

PDBj

CYP137

>CYP137A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVLRSLASPA ALTDPKRCAS VVGVAAFAVR REHAPDALGG PPGLPAPRGF 00500051 RAAFAAAYAV AYLAGGERRM LRLIRRYGPI MTMPILSLGD VAIVSDSALA 01000101 KEVFTAPTDV LLGGEGVGPA AAIYGSGSMF VQEEPEHLRR RKLLTPPLHG 01500151 AALDRYVPII ENSTRAAMHT WPVDRPFAML TVARSLMLDV IVKVIFGVDD 02000201 PEEVRRLGRP FERLLNLGVS EQLTVRYALR RLGALRVWPA RARANTEIDD 02500251 VVMALIAQRR ADPRLGERHD VLSLLVSARG ESGEQLSDSE IRDDLITLVL 03000301 AGHETTATTL AWAFDLLLHH PDALRRVRAE AVGGGEAFTT AVINETLRVR 03500351 PPAPLTARVA AQPLTIGGYR VEAGTRIVVH IIAINRSAEV YEHPHEFRPE 04000401 RFLGTRPQTY AWVPFGGGVK RCLGANFSMR ELITVLHVLL REGEFTAVDD 04500451 EPERIVRRSI MLVPRRGTRV RFRPAR

Heme Docked

PDBj

CYP138

>CYP138A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSEVVTAAPA PPVVRLPPAV RGPKLFQGLA FVVSRRRLLG RFVRRYGKAF 00500051 TANILMYGRV VVVADPQLAR QVFTSSPEEL GNIQPNLSRM FGSGSVFALD 01000101 GDDHRRRRRL LAPPFHGKSM KNYETIIEEE TLRETANWPQ GQAFATLPSM 01500151 MHITLNAILR AIFGAGGSEL DELRRLIPPW VTLGSRLAAL PKPKRDYGRL 02000201 SPWGRLAEWR RQYDTVIDKL IEAERADPNF ADRTDVLALM LRSTYDDGSI 02500251 MSRKDIGDEL LTLLAAGHET TAATLGWAFE RLSRHPDVLA ALVEEVDNGG 03000301 HELRQAAILE VQRARTVIDF AARRVNPPVY QLGEWVIPRG YSIIINIAQI 03500351 HGDPDVFPQP DRFDPQRYIG SKPSPFAWIP FGGGTRRCVG AAFANMEMDV 04000401 VLRTVLRHFT LETTTAAGER SHGRGVAFTP KDGGRVVMRR R

Heme Docked

PDBj

CYP139

>CYP139A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRTYRTVRYP LGEALLALYR WRGPLINAGV GGHGYTYLLG AEANRFVFAN 00500051 ADAFSWSQTF ESLVPVDGPT ALIVSDGADH RRRRSVVAPG LRHHHVQRYV 01000101 ATMVSNIDTV IDGWQPGQRL DIYQELRSAV RRSTAESLFG QRLAVHSDFL 01500151 GEQLQPLLDL TRRPPQVMRL QQRVNSPGWR RAMAARKRID DLIDAQIADA 02000201 RTAPRPDDHM LTTLISGCSE EGTTLSDNEI RDSIVSLITA GYETTSGALA 02500251 WAIYALLTVP GTWESAASEV ARVLGGRVPA ADDLSALTYL NGVVHETLRL 03000301 YSPGVISARR VLRDLWFDGH RIRAGRLLIF SAYVTHRLPE IWPEPTEFRP 03500351 LRWDPNAADY RKPAPHEFIP FSGGLHRCIG AVMATTEMTV ILARLVARAM 04000401 LQLPAQRTHR IRAANFAALR PWPGLTVEIR KSAPAQ

Heme Docked

PDBj

CYP140

>CYP140A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MKDKLHWLAM HGVIRGIAAI GIRRGDLQAR LIADPAVATD PVPFYDEVRS 00500051 HGALVRNRAN YLTVDHRLAH DLLRSDDFRV VSFGENLPPP LRWLERRTRG 01000101 DQLHPLREPS LLAVEPPDHT RYRKTVSAVF TSRAVSALRD LVEQTAINLL 01500151 DRFAEQPGIV DVVGRYCSQL PIVVISEILG VPEHDRPRVL EFGELAAPSL 02000201 DIGIPWRQYL RVQQGIRGFD CWLEGHLQQL RHAPGDDLMS QLIQIAESGD 02500251 NETQLDETEL RAIAGLVLVA GFETTVNLLG NGIRMLLDTP EHLATLRQHP 03000301 ELWPNTVEEI LRLDSPVQLT ARVACRDVEV AGVRIKRGEV VVIYLAAANR 03500351 DPAVFPDPHR FDIERPNAGR HLAFSTGRHF CLGAALARAE GEVGLRTFFD 04000401 RFPDVRAAGA GSRRDTRVLR GWSTLPVTLG PARSMVSP

Heme Docked

PDBj

CYP141 !!!GA!!!

>CYP141A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTSTSIPTFP FDRPVPTEPS PMLSELRNSC PVAPIELPSG HTAWLVTRFD 00500051 DVKGVLSDKR FSCRAAAHPS SPPFVPFVQL CPSLLSIDGP QHTAARRLLA 01000101 QGLNPGFIAR MRPVVQQIVD NALDDLAAAE PPVDFQEIVS VPIGEQLMAK 01500151 LLGVEPKTVH ELAAHVDAAM SVCEIGDEEV SRRWSALCTM VIDILHRKLA 02000201 EPGDDLLSTI AQANRQQSTM TDEQVVGMLL TVVIGGVDTP IAVITNGLAS 02500251 LLHHRDQYER LVEDPGRVAR AVEEIVRFNP ATEIEHLRVV TEDVVIAGTA 03000301 LSAGSPAFTS ITSANRDSDQ FLDPDEFDVE RNPNEHIAFG YGPHACPASA 03500351 YSRMCLTTFF TSLTQRFPQL QLARPFEDLE RRGKGLHSVG IKELLVTWPT 0400

Heme Docked

PDBj

CYP142

>CYP142A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTEAPDVDLA DGNFYASREA RAAYRWMRAN QPVFRDRNGL AAASTYQAVI 00500051 DAERQPELFS NAGGIRPDQP ALPMMIDMDD PAHLLRRKLV NAGFTRKRVK 01000101 DKEASIAALC DTLIDAVCER GECDFVRDLA APLPMAVIGD MLGVRPEQRD 01500151 MFLRWSDDLV TFLSSHVSQE DFQITMDAFA AYNDFTRATI AARRADPTDD 02000201 LVSVLVSSEV DGERLSDDEL VMETLLILIG GDETTRHTLS GGTEQLLRNR 02500251 DQWDLLQRDP SLLPGAIEEM LRWTAPVKNM CRVLTADTEF HGTALCAGEK 03000301 MMLLFESANF DEAVFCEPEK FDVQRNPNSH LAFGFGTHFC LGNQLARLEL 03500351 SLMTERVLRR LPDLRLVADD SVLPLRPANF VSGLESMPVV FTPSPPLG

Heme Docked

PDBj 1.6Å

CYP142A1 WT

CYP143

>CYP143A1 C_1  1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTPGEDHAG SFYLPRLEYS TLPMAVDRGV GWKTLRDAGP VVFMNGWYYL 00500051 TRREDVLAAL RNPKVFSSRK ALQPPGNPLP VVPLAFDPPE HTRYRRILQP 01000101 YFSPAALSKA LPSLRRHTVA MIDAIAGRGE CEAMADLANL FPFQLFLVLY 01500151 GLPLEDRDRL IGWKDAVIAM SDRPHPTEAD VAAARELLEY LTAMVAERRR 02000201 NPGPDVLSQV QIGEDPLSEI EVLGLSHLLI LAGLDTVTAA VGFSLLELAR 02500251 RPQLRAMLRD NPKQIRVFIE EIVRLEPSAP VAPRVTTEPV TVGGMTLPAG 03000301 SPVRLCMAAV NRDGSDAMST DELVMDGKVH RHWGFGGGPH RCLGSHLARL 03500351 ELTLLVGEWL NQIPDFELAP DYAPEIRFPS KSFALKNLPL RWS

Heme Docked

PDBj

CYP144

>CYP144A1 C_1  1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRRSPKGSPG AVLDLQRRVD QAVSADHAEL MTIAKDANTF FGAESVQDPY 00500051 PLYERMRAAG SVHRIANSDF YAVCGWDAVN EAIGRPEDFS SNLTATMTYT 01000101 AEGTAKPFEM DPLGGPTHVL ATADDPAHAV HRKLVLRHLA AKRIRVMEQF 01500151 TVQAADRLWV DGMQDGCIEW MGAMANRLPM MVVAELIGLP DPDIAQLVKW 02000201 GYAATQLLEG LVENDQLVAA GVALMELSGY IFEQFDRAAA DPRDNLLGEL 02500251 ATACASGELD TLTAQVMMVT LFAAGGESTA ALLGSAVWIL ATRPDIQQQV 03000301 RANPELLGAF IEETLRYEPP FRGHYRHVRN ATTLDGTELP ADSHLLLLWG 03500351 AANRDPAQFE APGEFRLDRA GGKGHISFGK GAHFCVGAAL ARLEARIVLR 04000401 LLLDRTSVIE AADVGGWLPS ILVRRIERLE LAVQ

Heme Docked

PDBj 1.5Å

CYP144A1 WT

CYP145

>CYP145A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSSVLAPESD VDLFAGEVLA DPYPIYAALR SLGPVVWLPR HGFYVVVRYA 00500051 EAREVLNDHE RFVSGRGVGF NQQFNDVRSG SIIASDPPRH DILRSVLNER 01000101 LGPRALKDTE VMIRSRASDL VAEMAQRRSF DAVKDFAEVF PVQVVGELIG 01500151 LPEGSRGRLL QWANGAFNAF GPSGERTAAG LEAIAEQFDY IRTVANREQL 02000201 MPDSMGAAVY QAADEGIISE DDCLPLLSAY LTAGMDTTVN ALSAMLLLLS 02500251 TDPDQWQELR SSPSLAPSVV NEVLRIEAPA QLFSRVTATR VDLAGTRIEA 03000301 GERVAVIYAS ANRDEKKYPD PDRFDIRRNP AGHLAFGSGL HVCAGQFLAK 03500351 TELRAVLDAL IEQIETMTVG EPVRKINNVL RGLSSLPATF VPVQTSR

Heme Docked

PDBj

CYP146

>CYP146A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MQTTTAVGDL GNPDLYTTLD RHARWRELAA RDAMVWSEPG SSPTGFWSVF 00500051 SHRACAAVLA PSAPFTSEYG MMIGFDRDHP DKSGGQMMVV SEQEQHRKLR 01000101 RLVGPLLSRA AARKLSERVR TEVSGVLDQV LDGGVCDVAT AIGPRIPAAV 01500151 VCEILGVPAE DEDMLIELTN HAFGGEDELF DGMTPRQAHT EILVYFDELI 02000201 TARRERPADD LVSTLVTDDE LTIDDVLLNC DNVLIGGNET TRHAITGAVH 02500251 ALATVPGLLT GLQDGSADVD TVVEEVLRWT SPAMHVLRVS TDDVTINGQD 03000301 LPAGTPVVAW LPAANRDPAE FDDPDTFLPG RKPNRHITFG HGMHHCLGSA 03500351 LARIELSVVL RVLAERVSRV ELVKEPAWLR AIVVQGYAEL SARFTGR

Heme Docked

PDBj 2.1Å

CYP146As (P450OxyD) WT

CYP147

>CYP147B1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MASDTLLARI TDYANRPDPY PLYAELREAG PVVRQADGSY LIGTYHEIVA 00500051 LLHDPRMSAD PRSRTAPAPY EVTRKPSFLR LDDPEHHRLR NSAMRPFGPP 01000101 HSPGRVDSMR GEIVQLTKEL AEVFQEGRQI DVVDDFAYPL PVTVICRLLG 01500151 IPDKDEQLFQ DWTDTLVASA DIGPEGDTAE RDQAADQAQQ EMGQYLVQLA 02000201 EQRRGRPTGD MLSDLVNEPD PAARLSEEDL AANTILLFIA GHETTVNLIA 02500251 NGVLTLLRRP DQLDRLREDP SLLPRAVEEL LRYEPPVHMR ERVPLVDIDV 03000301 AGTTIPGGTS VILALASGSR DPMRFSEPDR FDPTRPDNQH VGFGSGIHLC 03500351 FGAPLARIEA EAALGALLPH LGTARLVQDP PPYRQNAMLR GPRHLPIQL

Heme Docked

PDBj

CYP148

>CYP146A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTASSGSSAP SSGPLLAAVQ GLWSGAALAD PHPIYEQIRG FANADGLVRL 00500051 PEWNTAFAVG HAATSAVLRS PAARSGEWDH GPSDGGKLLQ HMMLFRNGIP 01000101 HARLRGLVQK AFTPRVVEEQ RDLVRSLLDE LLSDMARAGG PVDLVAGLSG 01500151 PLPGRVIMRM LGLRGADEER FLGWSASVAE LLGGADRSPA LLARIEADAR 02000201 EMRGYFRDLA DELRVSPQPG LLSALAAVED GGERLSGDEL LSNAVLLLAA 02500251 GHETTSNLIP GGVLALSQQP GAWAALLNHP RHPGVADELL RHVSPVQLDG 03000301 RMLTEAQTVG ETPLPAGTPV QLLLAAANRD PQVFPDPERL DWDRPNASRH 03500351 LAFAAGPHYC LGASLARLEI AETFAALAER FPDLRVSAAP HYKANFVLRG 04000401 PQELWVTLG

Heme Docked

PDBj

CYP149 (Unfold)

>CYP149A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MIKQPIAIVG MGCRFPGANN PSEFWEILQN GVHKITKDPI DRPTQMTGWA 00500051 GFLDGIDKFD AAFFGIYSEE AIKMDPQHRL LLETSWEALE DAKLVPANLA 01000101 GTDTGVFIGL SGSEYPNLLI EDSTFNTTIG TLDCMLANRI SSYFDFQGLS 01500151 ITINTACSSV LVAIDSACQS LWNEDICLAL VGGTHLTFSP VIASRASLNA 02000201 MRPKIQAIVD DLLDRFAPRG EMEIIADFAT PLPAFAITKI LGLPIEDYQQ 02500251 LIRWSAKTVF IFDQPVSLEE YKEQNQILIE HRAYFAQKVA EYKRQPNDGL 03000301 ISQLANYNDN INALTEDEII STSILLIATS QESMKGLLSN GLLALLKHPQ 03500351 SLEYVRQNPG NIENIVEELL RYDSPIQYVS RRAIEDVEVS GKIIHRGEYV 04000401 VIYLGAVNHD PEYFSNPQQL DFSRRKPNLG FGGGLHYCVG MFLARLQVQI 04500451 ALNAMVQRFP DICLNTDKLD WCDSKISRRL KTLPVKFTPV A

CYP150

>CYP150A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSDFDTIDYF TDPALVPDPH PYFDHLRAQC PVVKEPHYGV LAVTGYDEAA 00500051 TVLKDNETFS SCIAVAGPFP PLPFTPEGDD ITDQITAHRP QMPMFEHMVT 01000101 MDPPDHTNAR SLLNRLLTPS RLKENEDFMW RLADEVLDDF VTDGRCEFLT 01500151 AFAKPFSLLV IADMLGVPEE DHDEFRAVLG APRPGANVGS LDHSDLVGTN 02000201 PLEWLDEKFI GYLEDRRREP RNDVLTALAT AKYPDGSTPP VIEVVRSATF 02500251 LFAAGQETTT KLLSASMRVL GDHPDVQERL RRDRSRIPVF VEEALRMDAP 03000301 VKSQFRLAKK NTKVGDMNVP AGTTMMVCPG AVNRDPNRFE HPHEFDLDRK 03500351 NVREHIAFGR GVHSCPGGPL ARVEGRVSIE RILDRMADIQ IDEELHGPPG 04000401 ARRYTYEPTF ILRGLTDINI TFSPVG

Heme Docked

PDBj 1.6Å

CYP150A6 WT