CYPSD - Bacreria1501-2000

Reference

Cytochrome P450 HomepageDavid R. Nelson, (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.AlphafoldJohn Jumper, Richard Evans, Alexander Pritzel, et al. - Highly accurate protein structure prediction with AlphaFold. Nature, 1–11, doi: 10.1038/s41586-021-03819-2 (2021)ColabfoldMirdita M, Ovchinnikov S and Steinegger M. ColabFold - Making protein folding accessible to all. bioRxiv, doi: 10.1101/2021.08.15.456425 (2021)Localcolabfoldhttps://github.com/YoshitakaMo/localcolabfold

CYP101-CYP299, CYP1001-CYP1050Cytochrome P450 HomepageComprehensive Bacterial P450sDavid R. Nelson, (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65.https://drnelson.uthsc.edu/comprehensive-bacterial-p450s/
CYP1041, CYP1042, CYP1043, CYP1044, CYP1047, CYP1051-Fanele Cabangile Mnguni, David R. Nelson, and Khajamohiddin Syed et al.,More P450s Are Involved in Secondary Metabolite Biosynthesis in Streptomyces Compared to Bacillus, Cyanobacteria, and MycobacteriumInt. J. Mol. Sci. 2020, 21(13), 4814; https://doi.org/10.3390/ijms21134814
CYP1067, CYP1100, CYP1119-CYP1134Mohammad Parvez, David R Nelson, Khajamohiddin Syed et al.,Molecular evolutionary dynamics of cytochrome P450 monooxygenases across kingdoms: Special focus on mycobacterial P450s Sci. Rep. 6, 33099; doi: 10.1038/srep33099 (2016).
CYP1094, CYP1136, CYP1146, CYP1156, CYP1158-1159, CYP1177, CYP1184, CYP1185CYP1137, CYP1320, CYP1136, CYP1719, CYP1762, CYP1851, CYP1912, CYP1976, CYP2029, CYP2287, CYP2337, CYP2725Makhosazana Jabulile Khumalo, David R Nelson, Khajamohiddin Syed et al.,Comprehensive analyses of cytochrome P450 monooxygenases and secondary metabolite biosynthetic gene clusters in CyanobacteriaInt J Mol Sci . 2020, 21(2), 656. doi: 10.3390/ijms21020656.

D. R. Nelson's

Bacteria_1

Bacteria_2

Bacteria_3

https://twitter.com/P450nelson/status/1501396401317883904

Method

Localcolabfold (model1_ptm, recycle=6 or CaRMSD < 0.4, centerd and rotated around proximal Cys)runner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -alirunner_P450align.py -f CYPs/CYPXXXA1.fasta -nm 5 -mr 6 -ali -upMaestro (Protein preparation Wizard)proPKa pH 7.0, save as pdbHeme was extract from proximal-ligand-aligned crystal structure of CYP101A1-CYP150A1Heme was added hydrogen using maestro builder, save as mol2ES_scoreMaestro output

runner_P450.py

align_P450.py

CYP1509

>CYP1509A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1509A2 (2563685704)Streptomyces rapamycinicus (partial)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRAVASGLID RMADRNPFDL VADFTHLYPL EVLCALMGVP EEDIGRFSGW 00500051 TVDLGLLARF PLTPHLARID AAILGLRTYF RELVELRRRQ PDEDFVSTLI 01000101 AAQTETGPLT EDELHGALLN LLFAGHDTTR YQFGWVVQLL LEHGEWERVC 01500151 TEPDLVPGAV EEAMRLEPAL HIMLRKAVDA VEYHGLTLPA GTLLAVNIYA 02000201 TNRDPAMFPD PHRFDVTRAN AHRHLGFGHG GHLCLGHALA RAEMAEALAL 02500251 FVRWLPDLRQ AGDPDYPEGL SSMSGAERIP LTNRPQR
>CYP1509A? WP_167500819.1 [Streptomyces malaysiensis] A2 to 95%---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSEAAPPALT EVPRVDLADE RFRTNPGTAF DLIRQQSWII QTLRGYEVLS 00500051 YDVARQMCVD PRLDSIGPSY YENLAASEPI MWYATQASLP MIGGMRHDRI 01000101 RGALQRGFTR RRIDSLRPRM RAVASGLIDR MADRNPFDLV ADFTHLYPLE 01500151 VLCALMGVPE EDITRFSDWT VDLGLLARFP LTQHMARIDA AILGLRTYFR 02000201 ELVELRRRQP GDDFVSTLLA AQTETGPLTE DELHGALLNL LFAGHDTTRY 02500251 QFGWVVQLLL RHGEWERVCT EPGLVPGAIE EAMRLEPALH IMLRKAVDAV 03000301 EYHGLTLPAG TLLAVNIYAA NRDPAMFPDP HRFDVTRANA HRHLGFGHGG 03500351 HLCLGHALAR AEMAEALALF VRWLPDLRQA GDPDYPEGLS SMSGAERIPL 04000401 TNHPQQ

Alphafold2

.fasta

Heme Docked

PDBj

CYP1510

>CYP1510A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1510A2 (2545383125)Streptomyces rimosus rimosus ATCC 10970---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAAHADEPIR LAVGELARFL GADVDDPAFL TDPHRCLTPG IREKSVHRLP 00500051 GGALAVLGYT ACAEVLRDTR FGHGARELYE TTLLGLPARS FLQLDAPGHT 01000101 RLRGQVARHF TARRVRALAE NVGYYSTALV REHAGRPGDF VADFAEPLAM 01500151 SVISDVLGVP PEDRPAFHRD ARLVVRGLDQ PARAMDERAV AQARFRFVRF 02000201 FRRRAQARRQ AGTRHRAPRD GLLDALSHRP DGSPADIREL VTTCSLLLSA 02500251 GYDTTVSLLS HAVAELGGAP SGQGWALARD PQTVGAVVEE VLRLHSPVQI 03000301 APRAAVRDAA LDGLPVARGT IVLPLLPAAN RDPDIFDSPH TFRPRRYLAP 03500351 AAQGRSTARH LAFGAGAHFC LGAALARLTA HSALAVLAAC PPRPRDAPRT 04000401 YSEGVVVRGL RSLPVTWPHR PAERPPHPPA DGAAAYVEPS CPIPHAQRSV 04500451 T

Alphafold2

.fasta

Heme Docked

PDBj

CYP1518

>CYP1518A1 (648872783)Streptomyces sviceus ATCC 29083---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSATVPTMPG ALPFIGHGLH LLRDPLPLMR KANTLAPVVR LKGPGPDVYL 00500051 VTDPDLLHRM LVTDTRDYAK GRITDGAAPQ FGPSLLMDIH FDGLTLFEAH 01000101 RRHRRALQPA FHPRRTAAQV PQVRELTQTH CRHWQAGQTL RLDQDLARLA 01500151 YDVTARTFCA ATDSMAGALA ALTASSTTGV YWRVALPRLG RLSGVPGTGS 02000201 FKRAVARLRA AVTGAIEQHR ADPRDRGDVL SALLSARYHD TGEAMSDEQI 02500251 VNEITFYMFA AVHGIADVLP HVFLELSRHP DAEERLHAEL DTVLSGRPVQ 03000301 AEDLPRLHYT RRLLTEVMRL HPTVWLLARR TLRTVRLGTT DFPAGTEIAY 03500351 GAYAAHHHPG IHHDPLRFDP DRWLPERTRA LHKVAQLTFG TGPRKCIGDQ 04000401 LATTHMLTAL ATITQHWRLH TPPGHRHRPT PRLFLRPGTL PALITQRTPM 04500451 RTTSAAPAQS

Alphafold2

.fasta

Heme Docked

PDBj

CYP1524

>CYP1524A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1524A2 (2547558357)Streptomyces acidiscabies 84-104---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTVTPGFDLT ASSFHRNPYP VYRHLRTLGP VVRTAHRAWI ATHFTEVSAL 00500051 LRDARLRADF PADPRWVERH GGEDSVMVAD GRRWMLLRED TGHQEVRRVF 01000101 QRYFTPAAVE RRAERMTETV DAVLSGLDVT RPVDVVSEVA EPVVTSTMCD 01500151 LLGLPVHRRE RYAGWTSDIS RLTDPVPGHR VRERAETAMR EFREHIEALG 02000201 RQGRLTGIAA EVLYGSPSPA DVTANLVMLA MAGTDTTVSQ ITLAVHALFA 02500251 HPDQLEQAVR EPDSALRGVA EFARYDGPVQ LVTRRTSRPV RIGGTEIPAN 03000301 AKVMLCLGAA NHDPARYPAA HRLDLTRTGV ATLPFGDGPH YCLGARLGRL 03500351 ITGTLLSALL RRHPSLSPAA PLDRLPWRRS VVARRPEHLP VLLDGHTPAG 04000401 SRPLPRHDLY DDEGRRNSHG AH

Alphafold2

.fasta

Heme Docked

PDBj

CYP1529

>CYP1529A1 (2562119625)Streptomyces sp. CNQ-525---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MVRMYFGSRL FLYVSDPALV HEVLHVQNDE FEKGALFEKM KPLAGEGVGL 00500051 TSGDDHRMQR RMLRPSLQPS RLERYGEVMH DLALRCVDAW PRGQTVRVDR 01000101 EMVDYTIRVI VGTLLPAQSH PDLADRVRKA VPEVLYGLIR YLVLPELLFR 01500151 LPLPANKRAR LAVEQLKGTI EELVASYCEN PPAPADRFDL LSHMLDYRIP 02000201 ETGERVPRKL MRDEVMSLMI AGHETTSSAL TSIFWLMDRH AHMRERIHHE 02500251 VDAVGEGPVR LETLEQAPFT RSFVREVLRL YNPTWLLPRR ALRDVRLGRV 03000301 VVPAGTELII APMALHRRPD VFPRPHAFDP DRWRDGSTKG LPKGSYIPFG 03500351 LGPHVCMGAQ FAEMEIFTFL LAACRRWKLV QNPPAPARFR YGLIARIPEL 04000401 HCTVAPR

Alphafold2

.fasta

Heme Docked

PDBj

CYP1530

>CYP1530A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1530A4 (2521774701)Streptomyces canus 299MFChir4.1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MHVPSATPRQ HDEDGEDGRI RSRLTVSDLP EPPGLPLLGH LPALARGGTM 00500051 HRTLSDWRDR YGPTFRVRLG RKDAVVTSAP AILDTVLRER PDTFRRARSL 01000101 SDLIAELGAS GLFDSEGADW QRLRRMATRG LNSGQLRDSY SAIARSTGRL 01500151 RERWEAMAGA RVPVLEDLMR YTLDVTVALT MGHDLDAVRR ADEDGLHQRL 02000201 PLVFDTLTKR MNSPFPYWRW MRLPGDRRVD AVVAEAGALI RELYADSRHR 02500251 MGAGEKPRTY LDSLAMASLD NDEHITEGDV IGTILNMMIA GEDTAAATTA 03000301 WAIHHLAGHP EVQQRVRTEA EAVLGSDGYP AEPADLARLP YAGAVVNETI 03500351 RLNPVSPFIL LEAVTDTTVS DGTTDLHIGR GTVIVVLLTH GSDRDPLRYP 04000401 DPGRFEPERW LSSGARTSPQ EQPFMPFGGG PRFCPGRNLA LMESTLVVAM 04500451 TCHRFTVEPD TSAGPVGERV TLAVLPTHLN VRLHPASASA Q

Alphafold2

.fasta

Heme Docked

PDBj

CYP1536

>CYP1536A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1536A2 (ARV57260.1)Nostocales cyanobacterium HT-58-2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNTILQQQKN HHRQALRPPL AKGLPVLGNA LDLAGDVGDF LRKKYLELGP 00500051 VFRVRALNRE MVVLAGREAN LFFSKEGTKY FASRAVWQDF NAQLGISRSL 01000101 INMDGPDHTK YRNQLKPGYS VAMLEQNLDQ AVAIVRQEVA TWSTIKPQPV 01500151 FNLMQRIIIQ QLGLLITNYS PHEYLDDLIL FSRILTATTI GKQRPKFLTY 02000201 LPAYQRAKAR VFELARKVIA THSTELRGDR HPDLIDHLLQ FVKQEPEFLS 02500251 KQEDRINAAM TPFLAGVDTA ASVTAFLLYS LLKHPDILEQ ATVEANALFA 03000301 NDTLTPDNLR KLDVLQRVTL ETLRMYPIAP VLFRSVTVPF EFAGYRIEAG 03500351 QRVLIAISLP HHLPEFYPNP FQFDIERYSK GREEHRQPGV FAPFGLGSHL 04000401 CLGMNFAQAL IPLNIATILH AVRLSMVPAN YQLQIDPIPT PSPNKKFQIR 04500451 VAEQLE

Alphafold2

.fasta

Heme Docked

PDBj

CYP1543

>CYP1543A1 (645980601)Streptomyces sp. AA4---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MQNTSELPVL DDLVRSAKGP WWEWIPDIGR QHRFSRLGGM VLVHRYEDCW 00500051 EVLRDPARFR EGVFAAIKRL EGVDPRFVER RSKSLLSLEG EDHQRLRRLA 01000101 LPVFRAPLMD PLRPRMRRIF SDMVDAAASG APLDAVSALT RQYPLSVICA 01500151 VLGVPTDDLE FFSEIAEAQV SGLSGSPDTF PAAMRAYDEL DRYLTELLQQ 02000201 RRERPTDDLL SNLVRAEENG DRFSREELIS LVSTLLVAGT DTTRTTLASV 02500251 IYLFAKYPEQ WDDLVANPEL VPSALDEVMR FAPTAFVIKR MALADVTLGG 03000301 LDIPAGTEVA TLVVMANRDP DVFADPDRFD IRRVTRRPNL GFGAGYRHCI 03500351 GNALAKAELE EALKVLVERF ERIEPAGAPS WRRPDNIQGP TALPVRLIPR 04000401 AADRQPETVS

Alphafold2

.fasta

Heme Docked

PDBj

CYP1562

>CYP1562A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1562A2 (2516450698)Streptomyces sp. CNR698---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MESGRSAVRP SPGTGNDQED ARGLVLRFLD GDVRADPYPL LNRIRKAGPV 00500051 WLGDGVVVLS SHAHCEAALH AAEDAPASAS ASAAGCPVSA ARLRSAVDRA 01000101 FPADAVTGLA PLVRSLVDDR LDSVATRGRL EAVSDLAHPV PMAVLSHLLG 01500151 LPAGDAQWLH RRASALAPAL DLGPAPTGAE TSGDTAARRA ETELAAYLAE 02000201 AVADRRRRTG GDDLLSRLIR ADEHGERLTD AEAVSAGLLL LASGYETTSA 02500251 LVSGGILALL RAPHEIDTLR RDPAHARHLV EETLRLDPPL QVVRRRAGTD 03000301 LDLSGTRVPR GTVTVLLLAA AHRDPAVAAS PDVFAPGGAS PHLAFGAGPH 03500351 HCPGAPLARL IARTVLVRFA QRALAPRFAL GSPSYRPTAA LRGLRALWVD 04000401 ADGFAPRDLP WQPPAA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1568

>CYP1568A1 (2516059064)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTRRNAPTQA SMGTIPGRRW LAGHALKMAE DAFAVMDSAR DCGDIAVLWL 00500051 GPKPAYVISH PGLIRRLLTA EDKNLDKGPL YENLAQLIGV SIGTLIGRAH 01000101 RARRRAVAPA YVPNVTIMAD TAMELASSWQ PGQVYDMATQ MRRYSFMVMA 01500151 RTLFAGAIPP AAEAEFVELL PVALTGIAKY TADPTGVLKR LPTRDNRRIK 02000201 HALATLKKIT EDGLANHRNG SGGQGGVLAA LIRARDPDTG QPLTAEEIRN 02500251 EAMVMMSAGS ETVAGVLANA CAILAWQPDI ARRLHAETDR VLDELPIVEA 03000301 RLPELEYAGR VVTEILRMYP PGALMSRRAI VDLDLGGHRI PAGAAVFFCP 03500351 YLLHRRADLY PDPDRFDPER WLPERAAALP YGAFMPFGAG AHICVGEAIA 04000401 WQEALIAVAT ISKLWQLRPA SDQPPRPVLA PTLGLDAVHV TVVPHPSRRQ 04500451 PFEPEETADG DRA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1571

>CYP1571A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1571A2 (645418144)Streptomyces griseoflavus Tu4000---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRVPVVGDLL RLRADLLNAI QYAASSGAEV SHVDAGSRHM VFVHRSAQAD 00500051 AVLSDIRTFG KPDHKNPLRL VLGDGLVSNP DHDDWLRRRR ALQPVYSRAG 01000101 VAAMRRTTAE VVAEHTARWA GRDRTRLSVY EEMLAFTLDT VSRCMFSRSG 01500151 DSVSGTVNPE TAAFLLDFVE RRIRKPIALP VGVPTRRNRR FAATLRSLDT 02000201 LVHGIVGERR ASGRHHGDLL DLLLEARVGE AGEPLTDTGV RDEVLTTILA 02500251 GFETTASALT WTLYLLACYP EVQDAVRREV RASGGGEALR GTLLESVMYE 03000301 SMRLFPPSPT IPRQAKRDTS LGSAEVPEGA LVMLNVAAVH RDPAVWERPD 03500351 SFLPERFAHG TPEKGSYLPF GAGGHMCIGK GFALMEMGLL LADVVDRFEI 04000401 SPRDSAGPEP KALITLRPRD GFGLTLTRL

Alphafold2

.fasta

Heme Docked

PDBj

CYP1578

>CYP1578A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1578A2 (2580424789)Streptomyces sp. PRh5]---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNRCPIDINY PSLEGAENPY PLYEWLREEA PVHQNPGTTD FLVTRYEDVT 00500051 AVARDYETFS SVGSRSLYNP FNETGDGKAT ESLIEADPPD HRVKRDFCGN 01000101 AFKPRQLSRR TGMITDICHE LIDKLAGRTR INFVADFAEP LPLLVLTDMM 01500151 GFSRADLEWL DGWSRVDNGG LSFLPPDVQE LQRVSAHGAT EHLTRTILRR 02000201 AEEPGDDELS GMIQRQIARD GALDLDYIRD NAVTLIRGGV ITTAHLISAT 02500251 MRELVVRPTR LSEVRKDFSR IPAVLEETLR MESPVQWAPR RATRDTSIGG 03000301 VAIPAGARVL IMLGAANRDE GEFAKADEFA PERANVHSNI AFGVGPHFCL 03500351 GAPLARLEGR IAFETFLTRV RDIQLVPGQK LDHAPSAQFR GLNELLIDID 04000401 LKADTDD

Alphafold2

.fasta

Heme Docked

PDBj

CYP1607

>CYP1607A1 (2547560627)Streptomyces acidiscabies 84-104---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VSEEASRRAT TAAASSGCPR HHAASGLPDG PRLTAAELHA MESERQLAAW 00500051 AEYAHTYGPV FTLHSEGAPP RVYVSDPAEV RRLFIADRTD WGARGTLYFR 01000101 PVIGPQALPY LTGEAHRAVR LLLAPPLHGD RVRALGPALD SIVTAVVQRL 01500151 RGRSLPLIRL AHEITLRLIV RVAFGPLEAA RQENCVHVLT EIMDLMYEPA 02000201 ARPDTGSADL LQSLARPVRE FQTLVGEEAA TARADPHSGR TDLLHHLATS 02500251 TPAQSDEHIR GHIMTLLIAG HDTTASALAW ALYQLELHPG IRRQVHAELD 03000301 EPVSSVTPSP SPEQIARLPY LTAVCAEALR HGSVVPAGLA RVVPENPGRA 03500351 GHQYPAGTEL VPAIHLIHHR PDLYPAPERF APERFLDHRP SGTHYLPFGT 04000401 GTRRCPGAAL AEFELPIALA RLLRTPGLRL TGADPGLRAV KNGPTMTTPT 04500451 SLRITLDPAP PSEGDRP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1618

>CYP1618A1 (2721237398)Streptomyces sp. SAT1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VTETAAPPAP AGRARPAAMP GLPYFGGIAR MRKDPIAYLS EASRRGPVVR 00500051 LGRIGRRSYY QVNDPDLVRT VMTAPYADIG MTRTNDVLGT LFGESVFTMQ 01000101 GEVWRKRRDE LQPAFRTSNL PELFGSAADE IDRTVSRLAP IAEARGSVDC 01500151 SAALLDLLQR MIVRMMFGVA VPETAAQLTR AFDYGLEYRQ RRRWAPAGLP 02000201 RWFPTPASRL FDDGVARLNA SITEIIDDHR AHPPERAGLL RMLMECRDEE 02500251 TGAGWTDDEL LAEVKTTFVA GWLTTATAAV WLFDLLGRHP RITAHVVDEL 03000301 DRLPSDRPLR YEDLHTLEYT EDTILEAIRL FPPGWLLSRR VRRTYTLGGH 03500351 RLGRDTVLLV SPYALHRDPA HWTEPERFDP GRFRHGTHGE AHRAAYLPFG 04000401 YGPRVCIGRG VAMMELKLIV ATLLRRFRFT LADTAPARLQ PVSFLRRADP 04500451 LVLGVAPR

Alphafold2

.fasta

Heme Docked

PDBj

CYP1634

>CYP1634A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1634A2 (2523209349)Streptomyces flavidovirens DSM 40150---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTTEAADATD RLVTAFDHHD PGYTPQTAER VNSEIREQGV TWSPAYGGIW 00500051 ILSRYADVRA ALTDWRTYSS ARGVHFPRAE GMPMFSPIDY DPPQQREIRD 01000101 RMAGPMTGDA VGAMVPELRR IVARLLAPVV ERGHGDLMAE FAEPFAIEVL 01500151 GVAFGLSETC RSRVREATRT MWTYISTDRD ASKFWPAFHA LLAEEMDRVR 02000201 RRPDGSYLAR LAAMRRDGRP LPDEELYSII VSFCVAGHDN TMNSITRLVH 02500251 TLAGDPALQR RLRREPRLRP AVAEEALRRW CPTDRFTRVT TREVTVAGTV 03000301 IPAGARVVLL FDAANRDPEK FPEPDEFNPD RGNSHQHLSF GHGIHHCMGV 03500351 HLARAEFAAV LDELAQQPLF DLDEPSELHF ENGRHIMFDR VSVRFRTGEE 04000401 R

Alphafold2

.fasta

Heme Docked

PDBj

CYP1658

>CYP1658A1 (2546764541)Streptomyces aurantiacus JA 4570---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSGEFPMSWK AVPLVSPSGP SPARCPAHAG QDDAERAEAG EGPAPGGHLE 00500051 ELRREPLGLL RRSAKESAHR LARLDTGPGA CLVVSDPDAV DVVLTDHDAA 01000101 FFKPKMERWK QVLGENVLTS EGPAWARSRR RSMRITGARY VRRAARVVAE 01500151 VTDERLRSWA GTGAVGVDGA ADSPRSPCIP GSHDIPDIHE EMRTLTLAAV 02000201 LRHLCGTDDD FDLRAFGRHL RTVMECIHAL ESAPAGAEPS ARVERAFAHA 02500251 AGELRAAVLR LVASHAGGDD LVGLLTAPPD TASDDGRAAE PLTPDQVCDE 03000301 VLAHLIAGHE STATALAWSL LLLSRDADVT RRVRTEIGTT VGGRAPGPAD 03500351 LAGMPLLQAV FTEALRLCPP AWTIMRATGR PCELSGVRLP AGAVLLASPY 04000401 SIQRSGRWFP RPDEFLPDRW LGDAAAGAPR YAYFPFGGGR RSCPGRMLAQ 04500451 VTAGVVLTRV LQGFRIEPVG EPPTADVGII LRPSPGTRLR AVPLRRESAG 05000501 QREESP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1694

>CYP1694A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1694A3 (2579970905)Streptomyces sp. Mg1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTGAEAGAAA GSGAGPGSGP GSGRGAARPA GVRTAVAPYA PPARTGRAES 00500051 VRALAARALS ARALPGFARP RAAHTSTAAL RDLRERHGGA PVLVRGPSGP 01000101 VLVILDPQDM RRFYAEPAGV LAPDPCAPGG ERRRLGAEVL AAGTPVHPSH 01500151 GPFLAALAEE ARPLTATGTL RIATARRAVA RAGRRIVLGD AAAGDEELAG 02000201 WLRRLRGEGD AMGPRPTRAR AVRAVQERAT ARIARYAAHA EPHTLAGLAR 02500251 RAAAGPDDLA GQAYRWLRAM DTVPATLLRA LLLLGAHPGE QEAAGAEARA 03000301 GGGGPGELPR LRACVRETLR LYPVVPDLIR VTRAATEWRG VRYPAGTSVL 03500351 LPALFHQRDP EHVPAAHAFV PCRWSDRGAD RDIRMAPFGH GAGRCPGDQL 04000401 GLLVTAALCA EVLRGHRVTG TRPVLDPLGP LPATLDPQGI RLTLTRR

Alphafold2

.fasta

Heme Docked

PDBj

CYP1719

>CYP1719A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1719A2 (AOX02516.1)Moorea producens---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNTYPSTNVL DLLRLLGNLA SGFIRNPGGF DLEKVLGGWI GDVIKRYGSK 00500051 NVILNFLLKK VLLVSGRDLS DHILQDSPNS QGYIEGNLKK DGMSFLAPNA 01000101 LTISHDQQWQ RLRPYNEGVL GTGCQHQYQQ AFLEQVHRAF SKPVSNIEDI 01500151 RKCMGQAMLG IVFGENVAPE RLIKDIQVLF SMVGNPIKRI LLGRFETKRL 02000201 EKFYETLEQL WEGSQASEKP CLLSMAHGIK PYTTQEELLQ QIPHWMFTFT 02500251 GSGTDLLART LTMITSRPEV LERVRQEIKE AGSVEQASTI AKLGYLEACL 03000301 LETGRLFPPV TRTFHITTAA DTFNHNRIPP DMEILHFFPI MQREKSLDPS 03500351 TDSFVPQRWL DPRDQDTSTY SNLFLRGSRT CPGRDLILFV CKSAIAILLD 04000401 QQQLTSKTNL LSRDPLPAYF QEQDIQFVNP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1722

>CYP1722A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1722A3 (2721237164)Streptomyces sp. SAT1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRVSLKGPQP SDAASPDVYE EAQYAERDMH AVWQELRDSG GLHRQRTPDG 00500051 QEFYAATRYA DVREVLGDPG TFSSAYSTML TVRGDGDVAA GAAIHLTDPP 01000101 LHSEIKRAFT PLMTVPATQR LEQGITDRMS ELFETVRGRE SFDFCEAMVT 01500151 LPMTITGQLM GIPQQEWTTT GRATVRAMGG VDSAGSEYQR RFDLFEAHTT 02000201 IMTVLGEVLE ANPSGDTVSG RCPVTAQVVG DEGRDVALLN AYAFLMGANP 02500251 TVPQTINQTL ILLASDDDLW SWFTRQERTD QRFVDEALRW ASPVNHVLRR 03000301 TTADTALAGT EVPAGSLVSA WIASANRDEA VFEEPYAFLP DRSPNQHLAF 03500351 GVGVHRCIGQ HVAALGMRIF FDLWRKNVRS VSLGGDPGHL ISNFLNGVVC 04000401 LPLTVTWK

Alphafold2

.fasta

Heme Docked

PDBj

CYP1759

>CYP1759A1 (645977388)Streptomyces sp. AA4---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAVRTPPDLS PEIGPLFARE RDDALYEFYH AFREQSPFAI PGLNMVCIGK 00500051 YDHVASGFRL PGLVKQDPGR RDGKHPGWRD QVGLAQFYQS LLWENGSRHR 01000101 RLRGLVNKAF TPKRVAALSD GIAQVVTDLY QDLESAGREA DLARDLCSRI 01500151 PMHVIGSLVG VPKADQFALL PHVLDFLLVI DPVHAAEAAA RAGEASEVLH 02000201 RYFLDLVAER RRSPRDDLAS ALIGVRDDTE ELSEAELVQT IFLLFAAGFE 02500251 TTSNMLGNGC VALLSRPDVV ARIRRDPEAG AHAATEEVLR YDPPLQFVSR 03000301 IAEEQLVIGD TRIEAGTEVV FLIGSANRDP DAYENPDAFD LDRPRPPLAS 03500351 FAFGSHFCLG AGLARLEGRI VFGSLFDRFP GLALAGKPVR RKTFNMNGYE 04000401 TIPVTLY

Alphafold2

.fasta

Heme Docked

PDBj

CYP1762

>CYP1762A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1762B1 (BBC26498.1)Pseudanabaena sp. ABRG5-30001 MIDNLWSREV NNIPLPPLVK GLPILGSSLE LAKDNLGFFV KQYHEVGAIF 00500051 RVRALNRQFT VIAGAEANQF VNQLGTEFLS GQDFWQDFCK ELGTDNLLIS 01000101 LDGDRHLQQR RFLQPSYSRN SIIHAFPETI QLISDLTANL RSGQRVQVLP 01500151 FFQQIICEQL GMLLADCSPS HYREDLVRFL QTALNVTVVK QLPSFLLWLP 02000201 SYQRSQKRVL ELAKLAIKKN RMAQTKRQKP NLIDDLIADS QQPHPLLSES 02500251 EMIAAAVGPY LAGLDTVANT CSFMLYALLK SPEIMAKVIQ EVDWLFQDGI 03000301 PNAEGLRGLS ALHGAVMETL RMYPVAAAIQ RYAIADFTFK GFRVDAGTHV 03500351 IIATTVPHFL PQYFPNPYTF DIDRFHPPRN EHKQNGAFAP FGVGAHLCLG 04000401 NGLAEVQIML TMAMLLHRFE FSLESPNYKI QPISNPTLSP SNRFYVRVQT 04500451 RSTQRQK

Alphafold2

.fasta

Heme Docked

PDBj

CYP1810

>CYP1810A1 Streptomyces mobaraensis NBRC 13819 (Partial)---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLRDDALFTA DPRRLRDAGA LVPERSGTLA PEDRAPVREL LTDAFRAQDF 00500051 GQLAADVREF TRHLLTAREG RTETQFVADL AVPVASYAAG ALFGGGLARR 01000101 DGFLDTCRDA ARGMTAGATP EEAEAGLRAR ALLGQMLRAE YRYAHGGSDR 01500151 RGGVLRYLRL HEAERAVALE ALTGALCALL FAALNSGPRL LGNALNALVR 02000201 TPGSLPRLAT VNGPRLTAAL DELIRYDSPF QAQDRAVTSA TTLGGRRLAA 02500251 GDRVRVLLGS AHRDPEVFPH PDLLDLSRTP NPHFAFGLGP HACLGVPLAL 03000301 LEARVLLTEF AAAHPDATPA RPGLREPHPT LRGFLTLPVA VSR
>CYP1810A? WP_185941773.1 [Streptomyces mobaraensis]---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSGNSAGPGS PAGALFDPLS PAFLQDPYQV YTKLRQEDPV HRHPESGLWV 00500051 ISRYEDCLTV LRDDALFTAD PRRLRDAGAL GPERAGTLAP EERAPVRELL 01000101 TDAFRAQDFG QLAADVREFT RRLLARQGAR TETDFVTDLA MPVASYAAGA 01500151 LFGGGLARRD GFLDTCRDAA RGMTAGATPE ETEAGLRARA LLGQMLRAEY 02000201 RYAHGGSDRR GGVLRYLRLH EAERAVALEA LTGALCALLF AALNSGPRLL 02500251 GNALNALIRT PGALPRLATA NGPRLTAALN ELIRYDSPFQ AQDRAVTAAT 03000301 TLGGRRLAAG DRVRVLLGSA HRDPEVFPHP DRLDLTRTPN PHFAFGLGPH 03500351 ACLGVPLALL EARVLLTEFA TAHPDATPDA PGRHEPHPTL RGFLTLPVAL 04000401 GR

Alphafold2

.fasta

Heme Docked

PDBj

CYP1813 ???

>CYP1813A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1813A2 (646973602)Streptomyces bingchenggensis---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTHRTHTTHE FNRFADVRAA LADPALVPEL PATDGASAGG PAGASVAWLR 00500051 ATVARFSSGE PHRRRRALVE AELARLEPAS LWRAVATGPR GEVRVRVVRA 01000101 LAEALGLPEP GAVAEAVTVV AGAYFGGTDA AADEAVARLV ARLASASADE 01500151 AALEAVANRI GLLVQACDAT AALVESCAGA ALAEAAGGDV PPARVLREDP 02000201 PVRTMRRIAA RATRVAGREI AEGDVVRLDL ATAQRAHPVP LTFGAPPRVC 02500251 PGRAHALALA DGLLQRPLTA FARLHHQAAT PLLLPNAWDY ASAAALAARG 03000301 FPAVGTTSLG VAAATGLPDG AAATVDATLA LARSLGRGSF LFTVDAEGGF 03500351 SDDVAEVAAL AHELYDAGAA GINLEDGRAD GTLAPVELHA AKIAAVKAAV 04000401 PALFVNARTD THWLGRQEDE TAARLASYEQ AGADGVFVPG LSDPDGIASL 04500451 TAALLVPLNI LYTPTGPTLA ELAALGVRRV SLGSLLYRRA LAAAVTAATA 05000501 VRDGRPTDLT APSYAEVQAL AEP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1824

>CYP1824A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1824A2 (2533914779)Streptomyces auratus AGR0001---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 GGREQLPSAT PPFADHGGSP GAAESAETMQ ERDLMDARAF AGGVPHDYFR 00500051 HLRDRPGLTR AHDAEGYPFW YLVRHADVVR ASRDHDTFRS GPSTMTSVRQ 01000101 DSTGLPLISF LDAPEHTRLR KLAFKGFTPA RLAALDKPVA AIVDRILDDC 01500151 ADAGAFDLAE DLAMQLPLEV LTELIGVPPD ERAQVVEWTR HTVNLGDPDY 02000201 HHSTPEKAVA AFGNLMAYFE ELLGRRRAAP ADDLFSVLLA AGNDTSGRLE 02500251 PREIALFATT LMGAGSETTY CSVTGGVLAL LDHPGQLAAL RADRTLIPLA 03000301 VEEIIRWVTP ATHFARQVAR DTEIAGQPVR AGERVVLWYT SANRDESAFD 03500351 RPERFDVGRQ PNPHISFGGG GPHVCIGRGL ATLELRLLLA ALIDRLPRLE 04000401 LTGTPVRSIT NFMNSFRHMP ARFT

Alphafold2

.fasta

Heme Docked

PDBj

CYP1832

>CYP1832A1 (2524960817)Streptomyces sp. TAA204---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTALLEARDS PIADYACGGL TRTVRLAGST PSKAEILHLR REHYEPTLAT 00500051 MGVPVWDLHD ESAVHLLCSE QTGAGPGGAA PATGAVGGMR VTVNGAGSGD 01000101 LYEDFARLRD VVGDDGDFLV FARQLVHPSC RGTDVSTVLA HAACLYWRAH 01500151 SPVRRVLFTT IAGGTPERPG PVFGATPVAP PVSLGPQARK VVLFAGDLNT 02000201 IIEQAAGRLE RTGWSVAERT NVPAAANACS PGTSPAAPMR LPAPNDTPAQ 02500251 PSSVYRRLRR ERPVCRVRMA TGDPAWLITR FDDARFVLAD PRFSKAALLE 03000301 PDSPRTRPGA LPPGTLFTTD PPEHTRLSGM VKSALSKDRI AAFRPVMERI 03500351 AAELTEAMSA AEEGQDADLV RDFARPLAMR VMCGLLGVPE EDAGRFASWA 04000401 GTVLTAGPAG GSEVERAQDE LLARTTELVL ERKAESDGAQ PGGDLISALL 04500451 GHDGDVAAAV SLVATLMSAG YETMVASVAN SVLFLLACGH GMPAPWPSDA 05000501 GATVEELLRL STFGDALRSR RALADVRIGE VLVRKGDVVL VSLASVNRDE 05500551 RAFTDPDRFA PGRRQPRHLA FGHGVRRCPA SELARVELGV AMERLVTCFP 06000601 GMRLAVPPGE VTLRTHASES SPEKLPVVLG RSAARGA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1859

>CYP1859A1---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1859A2 (2585313356)Streptomyces mirabilis YR139---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDDKTQTHNP ALPEFWLQKD IEAAFAEMRR EKAVHWHEEP PSDWFPEGGR 00500051 GFWSVVRFED VVEVSRDQET FTSDHGTEIV DMTPEMVHIF GGMLNMSGAA 01000101 HAHHRAIVNR VLTPRTVEAL SDTIRAHARR CIDRVAAQDG CDFMQDMVGD 01500151 FPAQIICELL GVPVDDRARL VALTGTALSA YGTAEAYNAM LEIIAYAESL 02000201 VALAREQVSD SASFLRKLLT AQVDGQHMSD HEIAVFFALL VTAGIETTAT 02500251 SIGQGMYALS LHPEQRRRWQ SDFQQLAPRA VEEIVRWVTP VRYFRRTATR 03000301 DTELAGQPIA KGDKVVMWYT SANRDESVIE RAGELDFART EERHVAFGGG 03500351 GPHFCLGAVL ARKEMTIFFE ELFARLPDIE VNGEPERLHS NFVNGLAALP 04000401 VRYTPA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1851

>CYP1851A2---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1859A2 (2585313356)Streptomyces mirabilis YR139---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MFMASYPIHS LAFVLVPSFT ILFLTISEDL SSFVDVALGS VGKWIELSGQ 00500051 RTEPFLDALL SHYEFVLFAA VLLYFFVRSL RMLFLYSLQL IEFVVRVLRF 01000101 VGDLLSELFW VNPRFRINRE FSYRSDTSFT LVSIFARFVK EVLLLPIDWI 01500151 RSLGINGVFG PDPLTESKHY RKTAFYPSLL QRLLVIKNKH ITVYFFLSMM 02000201 RLFVHRFTLP AWFPGVNIKE NSHGDEFPVM RQLHLLKRHD VEEVLERSED 02500251 FRVVYGPRMC DVTQNGHSSG NFLLGMQSTS PTYIRDISNM RLIFRRDDVH 03000301 RCRRIAADAS VDSFLAFESR VDVLRDLDGS KVLSLPKDLV LPVIKSFIEQ 03500351 YFGVRLPLQA ENADGTTEID FNFVWFSHLF NYIFYDLNGD DSREAALASA 04000401 VLLNQDLDRQ IAAVKQQSSK HLEPANTVLA RCLRLQESGT PGMDDLAIRI 04500451 NLTGFLVGAV LPLINTICQV IDELISRPRI LEQAVSTATS ATEGGQSFLN 05000501 PLQGFVLEAL RFSPGDPVIY RHCKTRTELV SASCRSSVSA NTLVMAWNSS 05500551 AMFDPKYVDQ PWQFNPTRPP RDYLHFGHMY HVCAGKYIVM SVIPAIVQEL 06000601 LSRYELFRIP GARGYPVKHG IMVSGFDVLV RPRGSAD

Alphafold2

.fasta

Heme Docked

PDBj

CYP1866

>CYP1866A1 (2530559103)Streptomyces tsukubaensis NRRL 18488---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MRHNESVPIA PGALPLLGHL GRIGAEPQEF FRQLGDIGPV VRIRLARRTA 00500051 YVVTSPALVR RILVGGRGEF DKGGPFFIPF TTLLGDGLAT EPDSVHRVHR 01000101 PLVQPAFHRS AMTGYMRRVT QSATRHIAEW DPHQIIEVDR EMTALTTGVL 01500151 TRSLFTDPVS SRCVDRFQRD LPIVLDGLGL RMGLPSIGRF PHPANRRFYR 02000201 AVQHTTDSIE TMIRHRKQSP EPHDDLLTLL LAPEPHADGV LNDELVRGHI 02500251 WDFMLGGIEP TAALLSWTLH ILATFPDHYA RVQEEIDTVL AGASPTYQDL 03000301 ARLPQLQNTL TEALRLYPPV WVLSRTTTRA CELDGHKLPQ GADVIFSSWA 03500351 LHRHSDSFED ADRFDPDRWQ PARVGRAQRD GFLAFGAGAR KCIGDVFGVA 04000401 EATAMLAVLL QNRRLQPVPG PRVVARRGMT LRPKGLRIAF APLTSPKPAQ 04500451 DPACAAAPPN PGTGLRAPTP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1882

>CYP1882A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1882B1 (645203432)Streptomyces ghanaensis ATCC 14672---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MAVVDQRPTA ARCPVTSGFK PFDQDGTYEF FSLARHEAPV FYNEETDYWV 00500051 VTRRQDVLSV FKDPARFSAA NVLDPLEAHP EELTRFLADN GFTVEPVQSN 01000101 TDRPKHGRIR QCAGQFLNPK RYRDYEPRIR ALVQEYVHGL RGKDEVDIVA 01500151 ELTHEMPARV VFMLLGVDDV DPLLIKRWAL NRATMIWGKP TRQEMIDAGH 02000201 ELNDFFHFCK GIVEDRKRAP RDDYPSRLLE LRGDDDSVLT ENEIVCLVFA 02500251 LLLAGHEAVT NGLTISILTL LEHREAWEEL VADPALIPRA VEEVLRHSTP 03000301 VLTWRRKALE DVELSGVTIP AGSKVLLSLA SANHDEDRYA RPSDFDIHRV 03500351 NARDHLSFGY GVHVCMGAPL ARAEMRIVLE ELVDAFPGME LVEGQQPEWT 04000401 RTMAHRGPQS LKVRLGGSDH G

Alphafold2

.fasta

Heme Docked

PDBj

CYP1912

>CYP1912A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1912A2 (AUB37424.1)Nostoc flagelliforme---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDIGTPLKTS SSQDTETCKI KFNPFATGFK EDPYTTYRLL RQHEPIHRAL 00500051 DMWVVTKYQD ITHVLASDAV SVNYIPQYII QQNAGDIPGI VELGEKAIVF 01000101 TDPPEHGRLR RLASQAFNTI SIQAHTFYIQ QLIETLIGPK INAESINFAQ 01500151 IAHQIPLLTI SHLLDIPDEY IGVLDENLHR VRLVLEPGLL TKLRIRRIED 02000201 DLQVCLELFL DIIQYRQSHL GEDLLSKLIQ ARIGEDKLSH RELAIACVMT 02500251 YVAGHETSKG LLGNGVLAFA THPHQWSIFR DGKVSSKQVV DEILRFDPPL 03000301 QQTVRLAVSN LQVGQQTIGK GEKMLLCIAS ANRDEDQFAN ADDFDITRDA 03500351 SSQIAFGHGM HNCIGQMLAR LEAQFLLSYL VKHVQQIELS STDYRWIDDG 04000401 FITRTLSYLP VNLKSA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1914

>CYP1914A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1914B1 (2516057843)Streptomyces scabrisporus DSM 41855---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSEDWSVTSV VRRLLSPAGL ADPYAIYREI RAAADAGEPT GRFLFRYVDV 00500051 REALYDRDLS SNRVSAILRP LSDAARAEND LLESTLRDIV VFRDRADHRR 01000101 LRKLLTSAFS PRMVEMARPM IERMTDDLLS DLGGSGGADL HRELTYPLPA 01500151 MVVAALLGIP EADRKAFQSW ALDIVLVVGS GSLTPQLAHR ANDSMGRMRE 02000201 LMAWLVERRR REPGPDLLSA MIASSDDGDR LSTEELYANS LFLMTAGHET 02500251 ATNLLGNALL TLLRHPDQLA MLTEDFSLLD DAIEEVLRFE GPVQIAARIA 03000301 DRDREVQGVP LKAGQPLIVM LGAANRDPNV FPDPDRFDIT RPASRHVAFA 03500351 HGAHFCLGAT LARAEMQVVL PRILTRFPKL QLAEDEIEWQ PTLDFRGPNR 04000401 LRVTL

Alphafold2

.fasta

Heme Docked

PDBj

CYP1917

>CYP1917A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1917A2 (2527274921)Streptomyces sp. CNS606---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MNSLDTLDTL DITDEQAYAE HGYPYREWDL LRREAPVFWY VRPGFEPFWV 00500051 VTRHEDVAHV SRHTKIFSSA QRVTVDTADA VEMLEAELEE RAKMFGHSPD 01000101 DPPALSYMDS PRHRHLRRIM APCFTPKAVS ELEDRFAELA ASYAEEFTRH 01500151 LDEHGDADVA RHLAARLPVA AICELVGAPV EDWDSIFQWT EGTTAAADPE 02000201 YQLEGEDAEA AFRRNITALN GYVANLVQER MAAADGTGTD VLSRLAAGRI 02500251 DGEPLAFHEI LYTVFNLLVA GIGTTRNATT GGLQALLEHP DQLRKLVDDP 03000301 SLLDRAVEEI LRWTTIAVHF VRTATEDTEL SGTRIRKGET VALWYPAANR 03500351 DAEVFDDPYR FDITREHNPQ TAFGGHGEHV CLGAHLARLE LRAVLRAVLP 04000401 LLPELEVARK PDSSVLHLQV AEIKRLIVRR KK

Alphafold2

.fasta

Heme Docked

PDBj

CYP1920

>CYP1920A1 (2541458778)Streptomyces sp. HPH0547---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MLLRAGATSV AEPLLPNAAG TVVTTTVPGA PARITASADS PPPSPDKEHA 00500051 WMYDRQPCPL ASAHSASAAS SAGRPHYPFP LRRGDRLAPE LHCLLEREPV 01000101 CPVTTNSGHR AWLVTGYALT RQALEDPHLS LVRTSRPHAP YQDFSQAPWW 01500151 VTGFRDLTRQ AGLLPELVRA MGPAHCEVTA AWLRTTADNL LADLKGHGPP 02000201 ADLWSDFAVP LASRTVCRLL GVPARNGPFL VDCVERDLIL QDPSTAGRQP 02500251 AEDGEGPVVR AAVNIDDWVR EWMRHPETDP GGLLARLVRI NDRRRRLSDH 03000301 RFGSALNVLI VGAVGNPMAF LGTACFLLLQ HPALAESLRS DPEQVPRAVE 03500351 ELLRRSVLVG DGLARVARHP TTLGRARVDR DDLVLLNPDT ACYDPAVFPD 04000401 PERFDMNRGP VPQPTFGYGR NRCPGSHLAR LQARAALHAL LPHLGHLRLA 04500451 VPSDHVPWEN RSAVLRPTAL PVTWRARAGA DLPVQYAAAL PAPPAGGLLP 05000501 RTRTFPGVRR TSPGARGSKG SKGSTDRFVS GRGPRARPAQ HGRGCPRRRT 05500551 HCWCRARRAR TARGRLRFRD GHGGVGRHRP LAPGERRVAV AGGARSGRRA 06000601 SVPRCLSGSR PGRPRVPLRR RRGVRRGLAA APRCRGPRQC GRGARAPAGC 06500651 PGRATRSRKA VP

Alphafold2

.fasta

Heme Docked

PDBj

CYP1928

>CYP1928A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1928A2 (2538659090)Streptomyces ipomoeae 91-03---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MGDTRTDSLM EPPALDGGLP LLGHTLAFAK DPVALLLDGH RRLGEVFVVR 00500051 IAGRRFTVLS GPAATDAVFR TSDETVGRRR AYRSMTPVFG EGMLFDASPA 01000101 DFDEQIALIL PALRGGAVEG YAPVMQREVE DYLSGWGPTG RVDLVEAMSE 01500151 LSVNIAVECL IGAEFRRRVG PDLVRLFRDL KSAGRASWLV HPHLPLPAFR 02000201 RRDRARSELI ELILDAARSI QRDGPEAGRF VSTLMSSRFR DGTAVPEEVA 02500251 ASLLLAIIVA GEHNTAALAS WTGALLLRNP EWLAAVLREQ EELSGDADGP 03000301 RAVHQDLRRM DVLRRCMIEA ERLHPPTTVL LRDAEQEFSY GSYRIARGSR 03500351 VMVSPTASHR LPEVFTEPER YDPDRFSAER AEHRGTPQPM LAFGGGRHRC 04000401 TGMAFAYQEI QIIWSCLLRG YELELVGDTP RPDYSAVTAV PRSPCWVRYS 04500451 RRGGPQDDGG AARCATT

Alphafold2

.fasta

Heme Docked

PDBj

CYP1929

>CYP1929A1 (2545385606)Streptomyces rimosus rimosus ATCC 10970---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDARVRHSPE AERIDTRGEP PVWRAELPDG STAWVASGYD AARQVLTDSG 00500051 FAKPAVRGGE RWTDYLAFTG KEVTDSIVRS MLNTDGDLHR QLRELGASAF 01000101 TPERVRETAD RAETLAETLL DEIAGRGRAD LVHEFAHPFA VRAITEHLGY 01500151 PPDFIRRALE LRRWGPSPLF DPPGSPDRAR YAADRTAMSE LLHDLVAFRR 02000201 GSPGPDAVSG MIARADAAGL DEGQLTSTLF LLLVSAYEPV ADFLTSSLYS 02500251 LWHRPDLLAD PARVADGLGE LLRYTSPLAA TMPRFATRPM ELYGAELAPG 03000301 DAVIVHLALA NRDPRRFTAP NRLDLDRETG QDLVFAHGPH FCLGSQFAVR 03500351 LCRTALGAVL RRLPGLAAAR PLDTLPWQRG STGGITHLHV TAGLTELPVT 04000401 FQPRHAVVS

Alphafold2

.fasta

Heme Docked

PDBj

CYP1931

>CYP1931A1 (2538650261)Streptomyces ipomoeae 91-03---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDLFRPLDDT TISDPQPMYH RLRSTEPVHW YEPLSAWVVS GYDDCLFALH 00500051 RTDLFSSDWR RAGAEIPDNL LSIQTLDPPE HGAVHRVLMD AYRRQDFAAI 01000101 RTKLEDLADE LLGKLSAEPS FDLVESFTAP LSFAAVAALF GVPDADEKNI 01500151 VAWSEAIVAA MDSGLTPEAA APGTRARDQL SAEISRWLDS DPDSGLLADL 02000201 RRAEAARSIS RDMILNTLRA MLHAGYAPTS RFIAASVLAL LRTPAAWKEL 02500251 QTTGVTESVL KELLRHSGPV QAVARVCAQD VELGGRVLRK GSDVILLIAA 03000301 ANRDPRQFPR PDELDFTRTP NHSLAFGWGA HACAGASLAR LTCAVALGAL 03500351 LRHTPQLRPA GEPVHWRHAT LRGLRTLPVS GGAA

Alphafold2

.fasta

Heme Docked

PDBj

CYP1940

>CYP1940A1 (645393092)Streptomyces hygroscopicus ATCC 53653---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTAVSASGRR GDARSLPARA GIFWDPRTPV HFDEALNTWN VFSRADVLRV 00500051 LNDRETFSAG YGLSEEDRLR AHPSLSGMWA AEGARHDDLR AVVAEPFRRT 01000101 VLDSLAREVR DMVSELLDDV VAVGTGRIEV VGALAEALPS RVICRVLGLE 01500151 LSYADRMRTW VEEMSRAASA TSELPPQPDL VRFFQELIEA RRARPGSGLV 02000201 DDLIAAQRSG CPVAGRPMTE HDLVGYFAMM LSAGVDTTAT SIGNAFLFLT 02500251 EYGCWERLGE QPELVPHAIE ETLRWYPAFP GVRRHVLADT AIGGQHVRAG 03000301 QWVTGWLTSA NRDPDKFPDP GRFDMHRRPN PHVSLGNGRH HCLGAPLARL 03500351 ELRILIEEAA ARLPGLRRDT GEPLTRSQWI VDPLKSLQMT FDTPDPEESG 04000401 ILER

Alphafold2

.fasta

Heme Docked

PDBj

CYP1941

>CYP1941A1 (2541458531)Streptomyces sp. HPH0547---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MEETVTQVDF DPGVPDPVAE LDALLAAGHR VVRSPRMNGA YLVLSHREAT 00500051 EVLEDPETYS SAYALGGPLS AGLPREIVEE LPGFLREPVS GVVDTDPPAH 01000101 GQLRTTVQRA YAGPRVNPLT AQITGLAREL ADGLVRHEQP DLVEHYALPL 01500151 VHGSMGAAMG LPADMVPQVA AWAQDMASLL SPDISLPDKK AAARRLCEYE 02000201 QWARGFCAGL PEDHPGALGV YVHGEPAAAA RPFSPANQVN NLLVHWVAGT 02500251 VTTCHAITTT AHLLLRDRKL WEQAVAEPET WAARALEEGL RHTAPHRGLV 03000301 RVTTRETTLG GTTLPAGAQV LPILTAANRD PEHVSEPQRF DPHRERPRDH 03500351 LAFGAGAHTC VGQHLARLEG RIALTALLVR HPGLRPADPD RQPPLMPNYF 04000401 FHGIAALPVT GLSPRAAG

Alphafold2

.fasta

Heme Docked

PDBj

CYP1943

>CYP1943A1 (2541391902)Streptomyces sp. HGB0020---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTGGLTPTEK AGKPTVDFDH HSREYVERGV EITHGLREKC PVAWSEQHGG 00500051 YWVATRHDDV TTVFKNDEAY SSENWQGRGP RQGVLHPPTP HKEGMMEEDP 01000101 PAFIAYRKPL TKYFSQPAIE QIRPEIEDYA SWAIDQVIES GEAELVMSIF 01500151 AAVPALLTNR FLGLPLKEAT THALAMQMAF SIPPDGTPED RAKADQLFSW 02000201 STGAFRSAAA ERRKNPTDDL ISLVANLRVD GELLDMEEVV GNSFLLMSGG 02500251 ISTTTALLAN VFTYFDEHPE TREWIKEDYS RLKTAGQEFL RYFSPVQGLA 03000301 RTATRQCTLG DVTIEEDERI WMAMGAANRD PEVFPNPDEL DLKRSPNKHV 03500351 AFGVGMHRCM GSHMAMAMFE IVTEQTLRRM PDLKIDRERS RRYQSTGVLN 04000401 GWAELTATFT PGKPNGSTLA L

Alphafold2

.fasta

Heme Docked

PDBj

CYP1959

>CYP1959A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1959B1 (2524599102)Streptomyces sp. CNQ865---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MDASTDRPGG PDLLDLPLPQ PRECPFGPPP EMAGLRERTP VVRVKCPTGI 00500051 TAWLVTRYDD VRDVLGDAER FSSRAGQAIH LMAHADPGRP VGDVEFSRMD 01000101 GADYQRFRRI IGPEVSTAQR MAELRPMVQD LVDERLDALA AAGPGADFYG 01500151 DFAVPVTTAA IGGLVGVPYE DRELFHNAAA VMFSGATSQA DVLKVMRPLH 02000201 EYLYKLVVTR RAAPADDSLS RIIARSEAGE RPFTDLELVG ICAALLVPGF 02500251 DTTATVMAHG LLTLLAHPAE LARLRADPSL IPSAVEEIVR FLGGAPGIVR 03000301 QATRDTEIGG HPVAEGEYVV LAMQAADRDG DAFPDPDRLD LGRRPNNHLG 03500351 FGYGTHQCFG QQTARLELTV TLETLLRRVP SLRLAVPLDD VPFKTGSIVY 04000401 GPAEVPVAWD AVLPAGKAG

Alphafold2

.fasta

Heme Docked

PDBj

CYP1972 (No Cys)

>CYP1972A1 (651286215)Streptomyces sp. Tu6071---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTASGRLPRS HVPALASLAV RVARDPVPYY RLLHREPGPV RVPGTAGGGD 00500051 GIWLVGRDAD VRAALAEPGL VADAATGDVP GLVDGAGLLA GEKVERAEGE 01000101 TGRATAAGHA PGRVLASADV RADAPPPHVA GVVARAGHVL AGRLGSRASA 01500151 DLVPEFCDWL PAVALGAALG LPYRRTEALR RWCRAGGPAP LPLPDALAVR 02000201 ARGAERATAY ALASLLGTLL TRPAHLAAVR DEPALIDAVW AETLRHDPPA 02500251 PYLVLRARER VRLPGGAVAA GERVLCLLGA AGWDPAGYPD PGRWDPYRAG 03000301 PAPVPAHPWP DLGLLTVRYG LPPLLGVRGL ALAPGFAPRA VGVRVRGPRR 03500351 LLVRLGG

Alphafold2

.fasta

Heme Docked

PDBj

CYP1976

>CYP1972A1 (ABW28553.1)Acaryochloris marina---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTGLSAGSLT AGLHNTLKQL EAKPRQWVES KLQSYSQTLF QRIFSKQTQA 00500051 FSQIPGPQPL FPIGNVWALQ QGKPIWQVLG EQGQQYGGLS LFWILARPSL 01000101 VLNDPDLIQQ VLLTAQQQTN PHSHEHRANQ ADFYKDIPRK ALRPILTDSH 01500151 PFVAKTAGSK WQHLVKNNPF NMTYFREWME TQIQPLQVFI ESRAEALVKQ 02000201 SETEMLPAYE VIQKLAFDGF SLATVGQVFP DHVFEQFNTL CATGTDRMNR 02500251 SSIANWMIPE EPKGKHYKEA SRQWFQRFSE VLETTQADPA GGSLLDWVLK 03000301 RGGSAFTPEQ MRNFCAGVYP GGAVSAPSGI TSTLHLLFQH PQALSALQHD 03500351 LDGLFKEPLT LERLENCIPV EQVLRESLRL WPPVPFFTRN TVGATHLAGH 04000401 EIPANTQIFI NNWFLHRLSP HWHDSDSFQP ERWDTKTLAE NDYGSDYFFP 04500451 FGRGERACIG QDFAKFFMRL TLATLLAKVE IEFGDQPHDQ EFYFGVSVPR 05000501 QLKARFILRS

Alphafold2

.fasta

Heme Docked

PDBj

CYP1984

>CYP1984A1 (651286215)Streptomyces sp. Tu6071---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 VNPEDHRNPA TTEASQVLGG LLAPGQDDPY PLYDWLRTHE PVHFAPQLGG 00500051 YVLTRFEDCS YVMKNPALFP APDAETFARF MPEQSRYDAF KVLVTSIVNA 01000101 NPPQHTRLRR VVGRAFTPRR VESLKSGVMT IARDHVAKVG ARSADGAVID 01500151 FHHELSVPVP LQVMARLLGL PLDDDRLLAD LIPRIINVVD PSSDTETLKD 02000201 ADVALHTLLD RLWELVERRR ARPADDLVSA LVAPDAAEPE TLTDDELKAV 02500251 FVALLTAGFE TSATALDIAV HTLLRRPEHR PGLVDFEGTS RFVDEVLRWD 03000301 APAPMSAGFR YAAEEVRFGE HVVPAGSPVR VLLGAANRDP AANPRPEVFD 03500351 PSREAPRSLA FGSGIHHCLG MNLARMEIAA VLMAIVEGLP ELRFAEEPVR 04000401 RRSLPLRDFS SFTVQLGG

Alphafold2

.fasta

Heme Docked

PDBj

CYP1994

>CYP1994A1 (2513524125)Amycolatopsis sp. 75iv2, ATCC 39116---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MSRDAVYCSA WRYTEVAQRE RQIFAMTTPV DSAEFFDPLN PRHVAEPDEF 00500051 MRASRKGCPV GKVSDHLYAA NTDDVVRAVF DDTGNFSNRG NFSVGPEDVR 01000101 LPFTVVTMAD PPGHTLLRSR LLKSLAPARL RRLTPKVENI VRTAVGALPA 01500151 SGRVDLYADY VHFIPSAVVY ALIGIPEPAW REVQTWSDAI VRVIPGPSHE 02000201 LPEFASLMGF LARLVEERRA RPEDRHEDVL DNLCFAEPGE AEMSTPEVVC 02500251 HVFQLVVAAT DTTRALITNC VYRLLDDRSQ WEAVVADRSL LPNAIEESLR 03000301 VDSPAQFMVR TAVEDVTLAG CPIPAGKKVY LNLQSANHDE ERWGDDSRTY 03500351 RADRPDAAAH LAFGKGIHAC IGAPLARIEA RVAIEALMDA FPGMRLAPDA 04000401 TWVKCEGVLT RRVESVPVRL DGGEAR

Alphafold2

.fasta

Heme Docked

PDBj

CYP1995 (NoCys)

>CYP1995A1 ---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----
>CYP1995B1 (2721032478)Streptomyces parvulus---- 1234567890 1234567890 1234567890 1234567890 1234567890 ----0001 MTAEPLSAEP LPADRTRTDP SPAGPAPAKR LTAVPPVEPA SPGSPPSEPP 00500051 ATEPTAAEPV VTEPAPAARP RAGLAEALVA RRNQLTRAAQ WFAGTEGDPY 01000101 GLLLRAGADD PARHEERVRE WGPLYRSAPL DTWVTAHRVV AEQVLTDPAF 01500151 DGTGPDGTPV GLDGLPLRSE LLSLDRATAA RLAPLTAFGG PLLWAREENC 02000201 VAQRAATYAR RLLEGCGEHF DLAEDFARRL PGPVLAEQFS LPDAAHEEFA 02500251 RVLPDCRHAL DGLVCPQPYA TARATRTAEA TVAALLARHL SEAGGGAGAD 03000301 TARAAAVLAV SAAETTAVLI VDTVRRLRAR TGAWERLTAD PALAAEAVRE 03500351 TLRTAPPVRL ETRTTRTETT LAGTELPAGA RVAVLMAAAG RDPGAPAGAA 04000401 PLGLAPDLHF ALSAPLITSI AGAALRTLAE TLPHLREAGE PVIRPRSPVL 04500451 LAPARFPVAK RESTPCAS

Alphafold2

.fasta